Job ID = 14521940 SRX = SRX7637522 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14529345 spots for SRR10972034/SRR10972034.sra Written 14529345 spots for SRR10972034/SRR10972034.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:25 14529345 reads; of these: 14529345 (100.00%) were paired; of these: 1087737 (7.49%) aligned concordantly 0 times 11819133 (81.35%) aligned concordantly exactly 1 time 1622475 (11.17%) aligned concordantly >1 times ---- 1087737 pairs aligned concordantly 0 times; of these: 4033 (0.37%) aligned discordantly 1 time ---- 1083704 pairs aligned 0 times concordantly or discordantly; of these: 2167408 mates make up the pairs; of these: 2066233 (95.33%) aligned 0 times 79340 (3.66%) aligned exactly 1 time 21835 (1.01%) aligned >1 times 92.89% overall alignment rate Time searching: 00:10:25 Overall time: 00:10:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3982449 / 13444080 = 0.2962 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:11:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:11:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:11:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:11:26: 1000000 INFO @ Sat, 15 Jan 2022 22:11:33: 2000000 INFO @ Sat, 15 Jan 2022 22:11:39: 3000000 INFO @ Sat, 15 Jan 2022 22:11:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:11:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:11:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:11:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:11:53: 5000000 INFO @ Sat, 15 Jan 2022 22:12:00: 1000000 INFO @ Sat, 15 Jan 2022 22:12:00: 6000000 INFO @ Sat, 15 Jan 2022 22:12:07: 7000000 INFO @ Sat, 15 Jan 2022 22:12:10: 2000000 INFO @ Sat, 15 Jan 2022 22:12:16: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:12:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:12:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:12:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:12:21: 3000000 INFO @ Sat, 15 Jan 2022 22:12:25: 9000000 INFO @ Sat, 15 Jan 2022 22:12:30: 1000000 INFO @ Sat, 15 Jan 2022 22:12:32: 4000000 INFO @ Sat, 15 Jan 2022 22:12:33: 10000000 INFO @ Sat, 15 Jan 2022 22:12:40: 2000000 INFO @ Sat, 15 Jan 2022 22:12:41: 11000000 INFO @ Sat, 15 Jan 2022 22:12:44: 5000000 INFO @ Sat, 15 Jan 2022 22:12:48: 12000000 INFO @ Sat, 15 Jan 2022 22:12:50: 3000000 INFO @ Sat, 15 Jan 2022 22:12:55: 6000000 INFO @ Sat, 15 Jan 2022 22:12:55: 13000000 INFO @ Sat, 15 Jan 2022 22:13:00: 4000000 INFO @ Sat, 15 Jan 2022 22:13:02: 14000000 INFO @ Sat, 15 Jan 2022 22:13:06: 7000000 INFO @ Sat, 15 Jan 2022 22:13:09: 15000000 INFO @ Sat, 15 Jan 2022 22:13:10: 5000000 INFO @ Sat, 15 Jan 2022 22:13:16: 16000000 INFO @ Sat, 15 Jan 2022 22:13:17: 8000000 INFO @ Sat, 15 Jan 2022 22:13:20: 6000000 INFO @ Sat, 15 Jan 2022 22:13:24: 17000000 INFO @ Sat, 15 Jan 2022 22:13:28: 9000000 INFO @ Sat, 15 Jan 2022 22:13:30: 7000000 INFO @ Sat, 15 Jan 2022 22:13:31: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:13:38: 19000000 INFO @ Sat, 15 Jan 2022 22:13:38: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:13:38: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:13:38: #1 total tags in treatment: 9459788 INFO @ Sat, 15 Jan 2022 22:13:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:13:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:13:39: #1 tags after filtering in treatment: 7118348 INFO @ Sat, 15 Jan 2022 22:13:39: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:13:39: #1 finished! INFO @ Sat, 15 Jan 2022 22:13:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:13:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:13:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:13:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:13:39: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 22:13:39: 10000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:13:40: 8000000 INFO @ Sat, 15 Jan 2022 22:13:50: 11000000 INFO @ Sat, 15 Jan 2022 22:13:50: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:14:00: 10000000 INFO @ Sat, 15 Jan 2022 22:14:00: 12000000 INFO @ Sat, 15 Jan 2022 22:14:10: 11000000 INFO @ Sat, 15 Jan 2022 22:14:10: 13000000 INFO @ Sat, 15 Jan 2022 22:14:19: 12000000 INFO @ Sat, 15 Jan 2022 22:14:19: 14000000 INFO @ Sat, 15 Jan 2022 22:14:28: 13000000 INFO @ Sat, 15 Jan 2022 22:14:28: 15000000 INFO @ Sat, 15 Jan 2022 22:14:37: 16000000 INFO @ Sat, 15 Jan 2022 22:14:37: 14000000 INFO @ Sat, 15 Jan 2022 22:14:46: 17000000 INFO @ Sat, 15 Jan 2022 22:14:47: 15000000 INFO @ Sat, 15 Jan 2022 22:14:55: 18000000 INFO @ Sat, 15 Jan 2022 22:14:56: 16000000 INFO @ Sat, 15 Jan 2022 22:15:04: 19000000 INFO @ Sat, 15 Jan 2022 22:15:04: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:15:04: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:15:04: #1 total tags in treatment: 9459788 INFO @ Sat, 15 Jan 2022 22:15:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:15:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:15:04: #1 tags after filtering in treatment: 7118348 INFO @ Sat, 15 Jan 2022 22:15:04: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:15:04: #1 finished! INFO @ Sat, 15 Jan 2022 22:15:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:15:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:15:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:15:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:15:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.10_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 22:15:06: 17000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:15:15: 18000000 INFO @ Sat, 15 Jan 2022 22:15:24: 19000000 INFO @ Sat, 15 Jan 2022 22:15:24: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:15:24: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:15:24: #1 total tags in treatment: 9459788 INFO @ Sat, 15 Jan 2022 22:15:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:15:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:15:24: #1 tags after filtering in treatment: 7118348 INFO @ Sat, 15 Jan 2022 22:15:24: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:15:24: #1 finished! INFO @ Sat, 15 Jan 2022 22:15:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:15:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:15:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:15:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:15:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637522/SRX7637522.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling