Job ID = 5791702 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,446,809 reads read : 12,893,618 reads written : 6,446,809 reads 0-length : 6,446,809 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:45 6446809 reads; of these: 6446809 (100.00%) were unpaired; of these: 412640 (6.40%) aligned 0 times 5164401 (80.11%) aligned exactly 1 time 869768 (13.49%) aligned >1 times 93.60% overall alignment rate Time searching: 00:00:45 Overall time: 00:00:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1355107 / 6034169 = 0.2246 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:35:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:35:05: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:35:05: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:35:10: 1000000 INFO @ Wed, 22 Apr 2020 10:35:16: 2000000 INFO @ Wed, 22 Apr 2020 10:35:22: 3000000 INFO @ Wed, 22 Apr 2020 10:35:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:35:32: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:35:32: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:35:32: #1 total tags in treatment: 4679062 INFO @ Wed, 22 Apr 2020 10:35:32: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:35:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:35:32: #1 tags after filtering in treatment: 4679062 INFO @ Wed, 22 Apr 2020 10:35:32: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:35:32: #1 finished! INFO @ Wed, 22 Apr 2020 10:35:32: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:35:32: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:35:32: #2 number of paired peaks: 27 WARNING @ Wed, 22 Apr 2020 10:35:32: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:35:32: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 10:35:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:35:34: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:35:34: #1 read treatment tags... cut: /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:35:39: 1000000 INFO @ Wed, 22 Apr 2020 10:35:44: 2000000 INFO @ Wed, 22 Apr 2020 10:35:49: 3000000 INFO @ Wed, 22 Apr 2020 10:35:54: 4000000 INFO @ Wed, 22 Apr 2020 10:35:57: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:35:57: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:35:57: #1 total tags in treatment: 4679062 INFO @ Wed, 22 Apr 2020 10:35:57: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:35:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:35:57: #1 tags after filtering in treatment: 4679062 INFO @ Wed, 22 Apr 2020 10:35:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:35:57: #1 finished! INFO @ Wed, 22 Apr 2020 10:35:57: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:35:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:35:57: #2 number of paired peaks: 27 WARNING @ Wed, 22 Apr 2020 10:35:57: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:35:57: Process for pairing-model is terminated! BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container cut: /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:36:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:36:03: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:36:03: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:36:09: 1000000 INFO @ Wed, 22 Apr 2020 10:36:15: 2000000 INFO @ Wed, 22 Apr 2020 10:36:21: 3000000 INFO @ Wed, 22 Apr 2020 10:36:27: 4000000 INFO @ Wed, 22 Apr 2020 10:36:31: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:36:31: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:36:31: #1 total tags in treatment: 4679062 INFO @ Wed, 22 Apr 2020 10:36:31: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:36:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:36:31: #1 tags after filtering in treatment: 4679062 INFO @ Wed, 22 Apr 2020 10:36:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:36:31: #1 finished! INFO @ Wed, 22 Apr 2020 10:36:31: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:36:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:36:31: #2 number of paired peaks: 27 WARNING @ Wed, 22 Apr 2020 10:36:31: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:36:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587261/SRX7587261.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。