Job ID = 5791685 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,569,031 reads read : 11,138,062 reads written : 5,569,031 reads 0-length : 5,569,031 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:45 5569031 reads; of these: 5569031 (100.00%) were unpaired; of these: 337340 (6.06%) aligned 0 times 4464849 (80.17%) aligned exactly 1 time 766842 (13.77%) aligned >1 times 93.94% overall alignment rate Time searching: 00:00:45 Overall time: 00:00:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1120210 / 5231691 = 0.2141 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:36:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:36:29: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:36:29: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:36:35: 1000000 INFO @ Wed, 22 Apr 2020 10:36:41: 2000000 INFO @ Wed, 22 Apr 2020 10:36:47: 3000000 INFO @ Wed, 22 Apr 2020 10:36:53: 4000000 INFO @ Wed, 22 Apr 2020 10:36:54: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:36:54: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:36:54: #1 total tags in treatment: 4111481 INFO @ Wed, 22 Apr 2020 10:36:54: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:36:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:36:54: #1 tags after filtering in treatment: 4111481 INFO @ Wed, 22 Apr 2020 10:36:54: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:36:54: #1 finished! INFO @ Wed, 22 Apr 2020 10:36:54: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:36:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:36:54: #2 number of paired peaks: 33 WARNING @ Wed, 22 Apr 2020 10:36:54: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:36:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:36:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:36:59: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:36:59: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:37:04: 1000000 INFO @ Wed, 22 Apr 2020 10:37:09: 2000000 INFO @ Wed, 22 Apr 2020 10:37:14: 3000000 INFO @ Wed, 22 Apr 2020 10:37:19: 4000000 INFO @ Wed, 22 Apr 2020 10:37:20: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:37:20: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:37:20: #1 total tags in treatment: 4111481 INFO @ Wed, 22 Apr 2020 10:37:20: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:37:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:37:20: #1 tags after filtering in treatment: 4111481 INFO @ Wed, 22 Apr 2020 10:37:20: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:37:20: #1 finished! INFO @ Wed, 22 Apr 2020 10:37:20: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:37:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:37:20: #2 number of paired peaks: 33 WARNING @ Wed, 22 Apr 2020 10:37:20: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:37:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:37:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:37:29: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:37:29: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:37:34: 1000000 INFO @ Wed, 22 Apr 2020 10:37:39: 2000000 INFO @ Wed, 22 Apr 2020 10:37:44: 3000000 INFO @ Wed, 22 Apr 2020 10:37:49: 4000000 INFO @ Wed, 22 Apr 2020 10:37:50: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:37:50: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:37:50: #1 total tags in treatment: 4111481 INFO @ Wed, 22 Apr 2020 10:37:50: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:37:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:37:50: #1 tags after filtering in treatment: 4111481 INFO @ Wed, 22 Apr 2020 10:37:50: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:37:50: #1 finished! INFO @ Wed, 22 Apr 2020 10:37:50: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:37:50: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:37:50: #2 number of paired peaks: 33 WARNING @ Wed, 22 Apr 2020 10:37:50: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:37:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587247/SRX7587247.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。