Job ID = 5791683 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,007,994 reads read : 18,015,988 reads written : 9,007,994 reads 0-length : 9,007,994 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:30 9007994 reads; of these: 9007994 (100.00%) were unpaired; of these: 566679 (6.29%) aligned 0 times 7257760 (80.57%) aligned exactly 1 time 1183555 (13.14%) aligned >1 times 93.71% overall alignment rate Time searching: 00:01:30 Overall time: 00:01:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2159821 / 8441315 = 0.2559 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:31:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:31:17: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:31:17: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:31:23: 1000000 INFO @ Wed, 22 Apr 2020 10:31:30: 2000000 INFO @ Wed, 22 Apr 2020 10:31:36: 3000000 INFO @ Wed, 22 Apr 2020 10:31:42: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:31:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:31:48: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:31:48: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:31:48: 5000000 INFO @ Wed, 22 Apr 2020 10:31:55: 1000000 INFO @ Wed, 22 Apr 2020 10:31:55: 6000000 INFO @ Wed, 22 Apr 2020 10:31:57: #1 tag size is determined as 75 bps INFO @ Wed, 22 Apr 2020 10:31:57: #1 tag size = 75 INFO @ Wed, 22 Apr 2020 10:31:57: #1 total tags in treatment: 6281494 INFO @ Wed, 22 Apr 2020 10:31:57: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:31:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:31:57: #1 tags after filtering in treatment: 6281494 INFO @ Wed, 22 Apr 2020 10:31:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:31:57: #1 finished! INFO @ Wed, 22 Apr 2020 10:31:57: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:31:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:31:57: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:31:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:31:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:32:01: 2000000 INFO @ Wed, 22 Apr 2020 10:32:08: 3000000 INFO @ Wed, 22 Apr 2020 10:32:14: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:32:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:32:18: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:32:18: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:32:21: 5000000 INFO @ Wed, 22 Apr 2020 10:32:28: 1000000 INFO @ Wed, 22 Apr 2020 10:32:28: 6000000 INFO @ Wed, 22 Apr 2020 10:32:30: #1 tag size is determined as 75 bps INFO @ Wed, 22 Apr 2020 10:32:30: #1 tag size = 75 INFO @ Wed, 22 Apr 2020 10:32:30: #1 total tags in treatment: 6281494 INFO @ Wed, 22 Apr 2020 10:32:30: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:32:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:32:30: #1 tags after filtering in treatment: 6281494 INFO @ Wed, 22 Apr 2020 10:32:30: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:32:30: #1 finished! INFO @ Wed, 22 Apr 2020 10:32:30: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:32:30: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:32:30: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:32:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:32:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:32:37: 2000000 INFO @ Wed, 22 Apr 2020 10:32:46: 3000000 INFO @ Wed, 22 Apr 2020 10:32:55: 4000000 INFO @ Wed, 22 Apr 2020 10:33:04: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 10:33:13: 6000000 INFO @ Wed, 22 Apr 2020 10:33:15: #1 tag size is determined as 75 bps INFO @ Wed, 22 Apr 2020 10:33:15: #1 tag size = 75 INFO @ Wed, 22 Apr 2020 10:33:15: #1 total tags in treatment: 6281494 INFO @ Wed, 22 Apr 2020 10:33:15: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:33:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:33:15: #1 tags after filtering in treatment: 6281494 INFO @ Wed, 22 Apr 2020 10:33:15: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:33:15: #1 finished! INFO @ Wed, 22 Apr 2020 10:33:15: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:33:15: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:33:15: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:33:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:33:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7587245/SRX7587245.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。