Job ID = 14520113 SRX = SRX7496418 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10421410 spots for SRR10823029/SRR10823029.sra Written 10421410 spots for SRR10823029/SRR10823029.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:04 10421410 reads; of these: 10421410 (100.00%) were paired; of these: 4971506 (47.70%) aligned concordantly 0 times 5252867 (50.40%) aligned concordantly exactly 1 time 197037 (1.89%) aligned concordantly >1 times ---- 4971506 pairs aligned concordantly 0 times; of these: 38380 (0.77%) aligned discordantly 1 time ---- 4933126 pairs aligned 0 times concordantly or discordantly; of these: 9866252 mates make up the pairs; of these: 5754395 (58.32%) aligned 0 times 3927631 (39.81%) aligned exactly 1 time 184226 (1.87%) aligned >1 times 72.39% overall alignment rate Time searching: 00:04:04 Overall time: 00:04:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 270105 / 5487412 = 0.0492 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:30:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:30:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:30:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:30:27: 1000000 INFO @ Sat, 15 Jan 2022 18:30:32: 2000000 INFO @ Sat, 15 Jan 2022 18:30:37: 3000000 INFO @ Sat, 15 Jan 2022 18:30:41: 4000000 INFO @ Sat, 15 Jan 2022 18:30:46: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:30:51: 6000000 INFO @ Sat, 15 Jan 2022 18:30:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:30:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:30:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:30:56: 7000000 INFO @ Sat, 15 Jan 2022 18:30:58: 1000000 INFO @ Sat, 15 Jan 2022 18:31:01: 8000000 INFO @ Sat, 15 Jan 2022 18:31:03: 2000000 INFO @ Sat, 15 Jan 2022 18:31:07: 9000000 INFO @ Sat, 15 Jan 2022 18:31:09: 3000000 INFO @ Sat, 15 Jan 2022 18:31:12: 10000000 INFO @ Sat, 15 Jan 2022 18:31:14: 4000000 INFO @ Sat, 15 Jan 2022 18:31:17: 11000000 INFO @ Sat, 15 Jan 2022 18:31:20: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:31:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:31:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:31:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:31:23: 12000000 INFO @ Sat, 15 Jan 2022 18:31:25: 6000000 INFO @ Sat, 15 Jan 2022 18:31:28: 1000000 INFO @ Sat, 15 Jan 2022 18:31:28: 13000000 INFO @ Sat, 15 Jan 2022 18:31:31: 7000000 INFO @ Sat, 15 Jan 2022 18:31:33: 2000000 INFO @ Sat, 15 Jan 2022 18:31:34: 14000000 INFO @ Sat, 15 Jan 2022 18:31:36: 8000000 INFO @ Sat, 15 Jan 2022 18:31:37: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:31:37: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:31:37: #1 total tags in treatment: 5180046 INFO @ Sat, 15 Jan 2022 18:31:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:31:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:31:37: #1 tags after filtering in treatment: 2908164 INFO @ Sat, 15 Jan 2022 18:31:37: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 18:31:37: #1 finished! INFO @ Sat, 15 Jan 2022 18:31:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:31:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:31:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:31:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:31:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:31:39: 3000000 INFO @ Sat, 15 Jan 2022 18:31:42: 9000000 INFO @ Sat, 15 Jan 2022 18:31:44: 4000000 INFO @ Sat, 15 Jan 2022 18:31:47: 10000000 INFO @ Sat, 15 Jan 2022 18:31:49: 5000000 INFO @ Sat, 15 Jan 2022 18:31:53: 11000000 INFO @ Sat, 15 Jan 2022 18:31:55: 6000000 INFO @ Sat, 15 Jan 2022 18:31:58: 12000000 INFO @ Sat, 15 Jan 2022 18:32:00: 7000000 INFO @ Sat, 15 Jan 2022 18:32:04: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:32:06: 8000000 INFO @ Sat, 15 Jan 2022 18:32:10: 14000000 INFO @ Sat, 15 Jan 2022 18:32:12: 9000000 INFO @ Sat, 15 Jan 2022 18:32:13: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:32:13: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:32:13: #1 total tags in treatment: 5180046 INFO @ Sat, 15 Jan 2022 18:32:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:32:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:32:13: #1 tags after filtering in treatment: 2908164 INFO @ Sat, 15 Jan 2022 18:32:13: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 18:32:13: #1 finished! INFO @ Sat, 15 Jan 2022 18:32:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:32:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:32:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:32:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:32:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.10_peaks.narrowPeak: No such file or directory BigWig に変換しました。 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:32:17: 10000000 INFO @ Sat, 15 Jan 2022 18:32:21: 11000000 INFO @ Sat, 15 Jan 2022 18:32:26: 12000000 INFO @ Sat, 15 Jan 2022 18:32:31: 13000000 INFO @ Sat, 15 Jan 2022 18:32:35: 14000000 INFO @ Sat, 15 Jan 2022 18:32:38: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:32:38: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:32:38: #1 total tags in treatment: 5180046 INFO @ Sat, 15 Jan 2022 18:32:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:32:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:32:38: #1 tags after filtering in treatment: 2908164 INFO @ Sat, 15 Jan 2022 18:32:38: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 18:32:38: #1 finished! INFO @ Sat, 15 Jan 2022 18:32:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:32:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:32:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:32:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:32:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496418/SRX7496418.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling