Job ID = 10223969 SRX = SRX7496416 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17126048 spots for SRR10823027/SRR10823027.sra Written 17126048 spots for SRR10823027/SRR10823027.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:50 17126048 reads; of these: 17126048 (100.00%) were paired; of these: 7943206 (46.38%) aligned concordantly 0 times 8857587 (51.72%) aligned concordantly exactly 1 time 325255 (1.90%) aligned concordantly >1 times ---- 7943206 pairs aligned concordantly 0 times; of these: 46334 (0.58%) aligned discordantly 1 time ---- 7896872 pairs aligned 0 times concordantly or discordantly; of these: 15793744 mates make up the pairs; of these: 8957516 (56.72%) aligned 0 times 6544414 (41.44%) aligned exactly 1 time 291814 (1.85%) aligned >1 times 73.85% overall alignment rate Time searching: 00:05:50 Overall time: 00:05:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 598317 / 9222263 = 0.0649 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:11:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:11:51: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:11:51: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:11:56: 1000000 INFO @ Fri, 16 Oct 2020 09:12:00: 2000000 INFO @ Fri, 16 Oct 2020 09:12:04: 3000000 INFO @ Fri, 16 Oct 2020 09:12:07: 4000000 INFO @ Fri, 16 Oct 2020 09:12:11: 5000000 INFO @ Fri, 16 Oct 2020 09:12:15: 6000000 INFO @ Fri, 16 Oct 2020 09:12:19: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:12:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:12:21: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:12:21: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:12:23: 8000000 INFO @ Fri, 16 Oct 2020 09:12:26: 1000000 INFO @ Fri, 16 Oct 2020 09:12:27: 9000000 INFO @ Fri, 16 Oct 2020 09:12:30: 2000000 INFO @ Fri, 16 Oct 2020 09:12:31: 10000000 INFO @ Fri, 16 Oct 2020 09:12:35: 11000000 INFO @ Fri, 16 Oct 2020 09:12:35: 3000000 INFO @ Fri, 16 Oct 2020 09:12:38: 12000000 INFO @ Fri, 16 Oct 2020 09:12:39: 4000000 INFO @ Fri, 16 Oct 2020 09:12:42: 13000000 INFO @ Fri, 16 Oct 2020 09:12:43: 5000000 INFO @ Fri, 16 Oct 2020 09:12:46: 14000000 INFO @ Fri, 16 Oct 2020 09:12:48: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:12:50: 15000000 INFO @ Fri, 16 Oct 2020 09:12:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:12:51: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:12:51: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:12:52: 7000000 INFO @ Fri, 16 Oct 2020 09:12:54: 16000000 INFO @ Fri, 16 Oct 2020 09:12:56: 1000000 INFO @ Fri, 16 Oct 2020 09:12:56: 8000000 INFO @ Fri, 16 Oct 2020 09:12:58: 17000000 INFO @ Fri, 16 Oct 2020 09:13:00: 2000000 INFO @ Fri, 16 Oct 2020 09:13:01: 9000000 INFO @ Fri, 16 Oct 2020 09:13:02: 18000000 INFO @ Fri, 16 Oct 2020 09:13:03: 3000000 INFO @ Fri, 16 Oct 2020 09:13:05: 10000000 INFO @ Fri, 16 Oct 2020 09:13:05: 19000000 INFO @ Fri, 16 Oct 2020 09:13:07: 4000000 INFO @ Fri, 16 Oct 2020 09:13:09: 20000000 INFO @ Fri, 16 Oct 2020 09:13:10: 11000000 INFO @ Fri, 16 Oct 2020 09:13:11: 5000000 INFO @ Fri, 16 Oct 2020 09:13:14: 21000000 INFO @ Fri, 16 Oct 2020 09:13:14: 12000000 INFO @ Fri, 16 Oct 2020 09:13:15: 6000000 INFO @ Fri, 16 Oct 2020 09:13:18: 22000000 INFO @ Fri, 16 Oct 2020 09:13:19: 13000000 INFO @ Fri, 16 Oct 2020 09:13:19: 7000000 INFO @ Fri, 16 Oct 2020 09:13:22: 23000000 INFO @ Fri, 16 Oct 2020 09:13:23: 8000000 INFO @ Fri, 16 Oct 2020 09:13:23: 14000000 INFO @ Fri, 16 Oct 2020 09:13:26: 24000000 INFO @ Fri, 16 Oct 2020 09:13:26: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 09:13:26: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 09:13:26: #1 total tags in treatment: 8584805 INFO @ Fri, 16 Oct 2020 09:13:26: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:13:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:13:26: #1 tags after filtering in treatment: 3941201 INFO @ Fri, 16 Oct 2020 09:13:26: #1 Redundant rate of treatment: 0.54 INFO @ Fri, 16 Oct 2020 09:13:26: #1 finished! INFO @ Fri, 16 Oct 2020 09:13:26: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:13:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:13:27: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:13:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:13:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:13:27: 9000000 INFO @ Fri, 16 Oct 2020 09:13:28: 15000000 INFO @ Fri, 16 Oct 2020 09:13:31: 10000000 INFO @ Fri, 16 Oct 2020 09:13:32: 16000000 INFO @ Fri, 16 Oct 2020 09:13:35: 11000000 INFO @ Fri, 16 Oct 2020 09:13:37: 17000000 INFO @ Fri, 16 Oct 2020 09:13:39: 12000000 INFO @ Fri, 16 Oct 2020 09:13:42: 18000000 INFO @ Fri, 16 Oct 2020 09:13:43: 13000000 INFO @ Fri, 16 Oct 2020 09:13:46: 19000000 INFO @ Fri, 16 Oct 2020 09:13:47: 14000000 INFO @ Fri, 16 Oct 2020 09:13:51: 15000000 INFO @ Fri, 16 Oct 2020 09:13:51: 20000000 INFO @ Fri, 16 Oct 2020 09:13:55: 16000000 INFO @ Fri, 16 Oct 2020 09:13:55: 21000000 INFO @ Fri, 16 Oct 2020 09:13:59: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:14:00: 22000000 INFO @ Fri, 16 Oct 2020 09:14:02: 18000000 INFO @ Fri, 16 Oct 2020 09:14:05: 23000000 INFO @ Fri, 16 Oct 2020 09:14:06: 19000000 INFO @ Fri, 16 Oct 2020 09:14:09: 24000000 INFO @ Fri, 16 Oct 2020 09:14:10: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 09:14:10: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 09:14:10: #1 total tags in treatment: 8584805 INFO @ Fri, 16 Oct 2020 09:14:10: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:14:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:14:10: #1 tags after filtering in treatment: 3941201 INFO @ Fri, 16 Oct 2020 09:14:10: #1 Redundant rate of treatment: 0.54 INFO @ Fri, 16 Oct 2020 09:14:10: #1 finished! INFO @ Fri, 16 Oct 2020 09:14:10: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:14:10: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 09:14:10: 20000000 INFO @ Fri, 16 Oct 2020 09:14:10: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:14:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:14:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:14:14: 21000000 INFO @ Fri, 16 Oct 2020 09:14:18: 22000000 INFO @ Fri, 16 Oct 2020 09:14:22: 23000000 INFO @ Fri, 16 Oct 2020 09:14:25: 24000000 INFO @ Fri, 16 Oct 2020 09:14:26: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 09:14:26: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 09:14:26: #1 total tags in treatment: 8584805 INFO @ Fri, 16 Oct 2020 09:14:26: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:14:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:14:26: #1 tags after filtering in treatment: 3941201 INFO @ Fri, 16 Oct 2020 09:14:26: #1 Redundant rate of treatment: 0.54 INFO @ Fri, 16 Oct 2020 09:14:26: #1 finished! INFO @ Fri, 16 Oct 2020 09:14:26: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:14:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:14:26: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:14:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:14:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496416/SRX7496416.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling