Job ID = 10223965 SRX = SRX7496414 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 5213248 spots for SRR10823025/SRR10823025.sra Written 5213248 spots for SRR10823025/SRR10823025.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:42 5213248 reads; of these: 5213248 (100.00%) were paired; of these: 4730730 (90.74%) aligned concordantly 0 times 381028 (7.31%) aligned concordantly exactly 1 time 101490 (1.95%) aligned concordantly >1 times ---- 4730730 pairs aligned concordantly 0 times; of these: 8832 (0.19%) aligned discordantly 1 time ---- 4721898 pairs aligned 0 times concordantly or discordantly; of these: 9443796 mates make up the pairs; of these: 9125814 (96.63%) aligned 0 times 256976 (2.72%) aligned exactly 1 time 61006 (0.65%) aligned >1 times 12.47% overall alignment rate Time searching: 00:00:42 Overall time: 00:00:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 36850 / 491241 = 0.0750 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:00:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:00:53: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:00:53: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:00:58: 1000000 INFO @ Fri, 16 Oct 2020 09:00:59: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 09:00:59: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 09:00:59: #1 total tags in treatment: 445845 INFO @ Fri, 16 Oct 2020 09:00:59: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:00:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:00:59: #1 tags after filtering in treatment: 335427 INFO @ Fri, 16 Oct 2020 09:00:59: #1 Redundant rate of treatment: 0.25 INFO @ Fri, 16 Oct 2020 09:00:59: #1 finished! INFO @ Fri, 16 Oct 2020 09:00:59: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:00:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:00:59: #2 number of paired peaks: 56 WARNING @ Fri, 16 Oct 2020 09:00:59: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:00:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:01:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:01:23: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:01:23: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:01:27: 1000000 INFO @ Fri, 16 Oct 2020 09:01:28: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 09:01:28: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 09:01:28: #1 total tags in treatment: 445845 INFO @ Fri, 16 Oct 2020 09:01:28: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:01:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:01:28: #1 tags after filtering in treatment: 335427 INFO @ Fri, 16 Oct 2020 09:01:28: #1 Redundant rate of treatment: 0.25 INFO @ Fri, 16 Oct 2020 09:01:28: #1 finished! INFO @ Fri, 16 Oct 2020 09:01:28: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:01:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:01:28: #2 number of paired peaks: 56 WARNING @ Fri, 16 Oct 2020 09:01:28: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:01:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:01:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:01:53: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:01:53: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:01:58: 1000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 09:01:59: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 09:01:59: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 09:01:59: #1 total tags in treatment: 445845 INFO @ Fri, 16 Oct 2020 09:01:59: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:01:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:01:59: #1 tags after filtering in treatment: 335427 INFO @ Fri, 16 Oct 2020 09:01:59: #1 Redundant rate of treatment: 0.25 INFO @ Fri, 16 Oct 2020 09:01:59: #1 finished! INFO @ Fri, 16 Oct 2020 09:01:59: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:01:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:01:59: #2 number of paired peaks: 56 WARNING @ Fri, 16 Oct 2020 09:01:59: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:01:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496414/SRX7496414.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling