Job ID = 14520082 SRX = SRX7496408 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 5601365 spots for SRR10823019/SRR10823019.sra Written 5601365 spots for SRR10823019/SRR10823019.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:23 5601365 reads; of these: 5601365 (100.00%) were paired; of these: 2636776 (47.07%) aligned concordantly 0 times 2845643 (50.80%) aligned concordantly exactly 1 time 118946 (2.12%) aligned concordantly >1 times ---- 2636776 pairs aligned concordantly 0 times; of these: 55509 (2.11%) aligned discordantly 1 time ---- 2581267 pairs aligned 0 times concordantly or discordantly; of these: 5162534 mates make up the pairs; of these: 2740963 (53.09%) aligned 0 times 2303306 (44.62%) aligned exactly 1 time 118265 (2.29%) aligned >1 times 75.53% overall alignment rate Time searching: 00:02:23 Overall time: 00:02:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 85859 / 3016913 = 0.0285 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:23:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:23:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:23:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:23:28: 1000000 INFO @ Sat, 15 Jan 2022 18:23:34: 2000000 INFO @ Sat, 15 Jan 2022 18:23:40: 3000000 INFO @ Sat, 15 Jan 2022 18:23:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:23:52: 5000000 INFO @ Sat, 15 Jan 2022 18:23:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:23:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:23:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:23:59: 6000000 INFO @ Sat, 15 Jan 2022 18:23:59: 1000000 INFO @ Sat, 15 Jan 2022 18:24:06: 7000000 INFO @ Sat, 15 Jan 2022 18:24:06: 2000000 INFO @ Sat, 15 Jan 2022 18:24:12: 8000000 INFO @ Sat, 15 Jan 2022 18:24:13: 3000000 INFO @ Sat, 15 Jan 2022 18:24:14: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:24:14: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:24:14: #1 total tags in treatment: 2879215 INFO @ Sat, 15 Jan 2022 18:24:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:24:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:24:14: #1 tags after filtering in treatment: 1984343 INFO @ Sat, 15 Jan 2022 18:24:14: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 18:24:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:24:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:24:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:24:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:24:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:24:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:24:19: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:25: 5000000 INFO @ Sat, 15 Jan 2022 18:24:29: 1000000 INFO @ Sat, 15 Jan 2022 18:24:32: 6000000 INFO @ Sat, 15 Jan 2022 18:24:36: 2000000 INFO @ Sat, 15 Jan 2022 18:24:39: 7000000 INFO @ Sat, 15 Jan 2022 18:24:42: 3000000 INFO @ Sat, 15 Jan 2022 18:24:45: 8000000 INFO @ Sat, 15 Jan 2022 18:24:47: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:24:47: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:24:47: #1 total tags in treatment: 2879215 INFO @ Sat, 15 Jan 2022 18:24:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:24:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:24:47: #1 tags after filtering in treatment: 1984343 INFO @ Sat, 15 Jan 2022 18:24:47: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 18:24:47: #1 finished! INFO @ Sat, 15 Jan 2022 18:24:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:24:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:24:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:24:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:24:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:24:49: 4000000 INFO @ Sat, 15 Jan 2022 18:24:55: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:25:01: 6000000 INFO @ Sat, 15 Jan 2022 18:25:07: 7000000 INFO @ Sat, 15 Jan 2022 18:25:13: 8000000 INFO @ Sat, 15 Jan 2022 18:25:15: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:25:15: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:25:15: #1 total tags in treatment: 2879215 INFO @ Sat, 15 Jan 2022 18:25:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:25:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:25:15: #1 tags after filtering in treatment: 1984343 INFO @ Sat, 15 Jan 2022 18:25:15: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 18:25:15: #1 finished! INFO @ Sat, 15 Jan 2022 18:25:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:25:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:25:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:25:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:25:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496408/SRX7496408.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling