Job ID = 14520072 SRX = SRX7496402 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 4282572 spots for SRR10823013/SRR10823013.sra Written 4282572 spots for SRR10823013/SRR10823013.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:38 4282572 reads; of these: 4282572 (100.00%) were paired; of these: 2388255 (55.77%) aligned concordantly 0 times 1809907 (42.26%) aligned concordantly exactly 1 time 84410 (1.97%) aligned concordantly >1 times ---- 2388255 pairs aligned concordantly 0 times; of these: 6675 (0.28%) aligned discordantly 1 time ---- 2381580 pairs aligned 0 times concordantly or discordantly; of these: 4763160 mates make up the pairs; of these: 2937462 (61.67%) aligned 0 times 1729271 (36.31%) aligned exactly 1 time 96427 (2.02%) aligned >1 times 65.70% overall alignment rate Time searching: 00:01:38 Overall time: 00:01:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 46799 / 1900774 = 0.0246 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:19:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:19:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:19:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:19:34: 1000000 INFO @ Sat, 15 Jan 2022 18:19:39: 2000000 INFO @ Sat, 15 Jan 2022 18:19:44: 3000000 INFO @ Sat, 15 Jan 2022 18:19:49: 4000000 INFO @ Sat, 15 Jan 2022 18:19:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:19:57: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:19:57: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:19:57: #1 total tags in treatment: 1847544 INFO @ Sat, 15 Jan 2022 18:19:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:19:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:19:57: #1 tags after filtering in treatment: 1388427 INFO @ Sat, 15 Jan 2022 18:19:57: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 18:19:57: #1 finished! INFO @ Sat, 15 Jan 2022 18:19:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:19:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:19:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:19:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:19:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:19:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:19:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:19:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:20:04: 1000000 INFO @ Sat, 15 Jan 2022 18:20:09: 2000000 INFO @ Sat, 15 Jan 2022 18:20:13: 3000000 INFO @ Sat, 15 Jan 2022 18:20:17: 4000000 INFO @ Sat, 15 Jan 2022 18:20:22: 5000000 INFO @ Sat, 15 Jan 2022 18:20:24: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:20:24: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:20:24: #1 total tags in treatment: 1847544 INFO @ Sat, 15 Jan 2022 18:20:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:24: #1 tags after filtering in treatment: 1388427 INFO @ Sat, 15 Jan 2022 18:20:24: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 18:20:24: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:20:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:20:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:20:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:20:33: 1000000 INFO @ Sat, 15 Jan 2022 18:20:38: 2000000 INFO @ Sat, 15 Jan 2022 18:20:42: 3000000 INFO @ Sat, 15 Jan 2022 18:20:47: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:20:51: 5000000 INFO @ Sat, 15 Jan 2022 18:20:54: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:20:54: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:20:54: #1 total tags in treatment: 1847544 INFO @ Sat, 15 Jan 2022 18:20:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:54: #1 tags after filtering in treatment: 1388427 INFO @ Sat, 15 Jan 2022 18:20:54: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 18:20:54: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496402/SRX7496402.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。