Job ID = 14520200 SRX = SRX7496399 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 22277173 spots for SRR10823010/SRR10823010.sra Written 22277173 spots for SRR10823010/SRR10823010.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:50 22277173 reads; of these: 22277173 (100.00%) were paired; of these: 12212011 (54.82%) aligned concordantly 0 times 9244079 (41.50%) aligned concordantly exactly 1 time 821083 (3.69%) aligned concordantly >1 times ---- 12212011 pairs aligned concordantly 0 times; of these: 34763 (0.28%) aligned discordantly 1 time ---- 12177248 pairs aligned 0 times concordantly or discordantly; of these: 24354496 mates make up the pairs; of these: 14398516 (59.12%) aligned 0 times 9059594 (37.20%) aligned exactly 1 time 896386 (3.68%) aligned >1 times 67.68% overall alignment rate Time searching: 00:08:50 Overall time: 00:08:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 671268 / 10088317 = 0.0665 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:55:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:55:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:55:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:55:56: 1000000 INFO @ Sat, 15 Jan 2022 18:56:00: 2000000 INFO @ Sat, 15 Jan 2022 18:56:05: 3000000 INFO @ Sat, 15 Jan 2022 18:56:10: 4000000 INFO @ Sat, 15 Jan 2022 18:56:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:21: 6000000 INFO @ Sat, 15 Jan 2022 18:56:26: 1000000 INFO @ Sat, 15 Jan 2022 18:56:27: 7000000 INFO @ Sat, 15 Jan 2022 18:56:31: 2000000 INFO @ Sat, 15 Jan 2022 18:56:33: 8000000 INFO @ Sat, 15 Jan 2022 18:56:36: 3000000 INFO @ Sat, 15 Jan 2022 18:56:38: 9000000 INFO @ Sat, 15 Jan 2022 18:56:42: 4000000 INFO @ Sat, 15 Jan 2022 18:56:44: 10000000 INFO @ Sat, 15 Jan 2022 18:56:48: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:50: 11000000 INFO @ Sat, 15 Jan 2022 18:56:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:54: 6000000 INFO @ Sat, 15 Jan 2022 18:56:56: 1000000 INFO @ Sat, 15 Jan 2022 18:56:56: 12000000 INFO @ Sat, 15 Jan 2022 18:57:00: 7000000 INFO @ Sat, 15 Jan 2022 18:57:01: 2000000 INFO @ Sat, 15 Jan 2022 18:57:02: 13000000 INFO @ Sat, 15 Jan 2022 18:57:05: 8000000 INFO @ Sat, 15 Jan 2022 18:57:06: 3000000 INFO @ Sat, 15 Jan 2022 18:57:07: 14000000 INFO @ Sat, 15 Jan 2022 18:57:11: 9000000 INFO @ Sat, 15 Jan 2022 18:57:12: 4000000 INFO @ Sat, 15 Jan 2022 18:57:13: 15000000 INFO @ Sat, 15 Jan 2022 18:57:17: 10000000 INFO @ Sat, 15 Jan 2022 18:57:18: 5000000 INFO @ Sat, 15 Jan 2022 18:57:19: 16000000 INFO @ Sat, 15 Jan 2022 18:57:23: 11000000 INFO @ Sat, 15 Jan 2022 18:57:24: 6000000 INFO @ Sat, 15 Jan 2022 18:57:25: 17000000 INFO @ Sat, 15 Jan 2022 18:57:28: 12000000 INFO @ Sat, 15 Jan 2022 18:57:30: 7000000 INFO @ Sat, 15 Jan 2022 18:57:31: 18000000 INFO @ Sat, 15 Jan 2022 18:57:34: 13000000 INFO @ Sat, 15 Jan 2022 18:57:36: 8000000 INFO @ Sat, 15 Jan 2022 18:57:36: 19000000 INFO @ Sat, 15 Jan 2022 18:57:40: 14000000 INFO @ Sat, 15 Jan 2022 18:57:41: 9000000 INFO @ Sat, 15 Jan 2022 18:57:42: 20000000 INFO @ Sat, 15 Jan 2022 18:57:46: 15000000 INFO @ Sat, 15 Jan 2022 18:57:47: 10000000 INFO @ Sat, 15 Jan 2022 18:57:47: 21000000 INFO @ Sat, 15 Jan 2022 18:57:52: 16000000 INFO @ Sat, 15 Jan 2022 18:57:53: 22000000 INFO @ Sat, 15 Jan 2022 18:57:53: 11000000 INFO @ Sat, 15 Jan 2022 18:57:58: 17000000 INFO @ Sat, 15 Jan 2022 18:57:58: 23000000 INFO @ Sat, 15 Jan 2022 18:57:59: 12000000 INFO @ Sat, 15 Jan 2022 18:58:03: 18000000 INFO @ Sat, 15 Jan 2022 18:58:04: 24000000 INFO @ Sat, 15 Jan 2022 18:58:05: 13000000 INFO @ Sat, 15 Jan 2022 18:58:09: 19000000 INFO @ Sat, 15 Jan 2022 18:58:09: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:58:10: 14000000 INFO @ Sat, 15 Jan 2022 18:58:15: 20000000 INFO @ Sat, 15 Jan 2022 18:58:15: 26000000 INFO @ Sat, 15 Jan 2022 18:58:16: 15000000 INFO @ Sat, 15 Jan 2022 18:58:20: 21000000 INFO @ Sat, 15 Jan 2022 18:58:21: 27000000 INFO @ Sat, 15 Jan 2022 18:58:22: 16000000 INFO @ Sat, 15 Jan 2022 18:58:25: 22000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:58:27: 28000000 INFO @ Sat, 15 Jan 2022 18:58:28: 17000000 INFO @ Sat, 15 Jan 2022 18:58:31: 23000000 INFO @ Sat, 15 Jan 2022 18:58:32: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:58:32: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:58:32: #1 total tags in treatment: 9394143 INFO @ Sat, 15 Jan 2022 18:58:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:32: #1 tags after filtering in treatment: 4766689 INFO @ Sat, 15 Jan 2022 18:58:32: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 18:58:32: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:58:34: 18000000 INFO @ Sat, 15 Jan 2022 18:58:36: 24000000 INFO @ Sat, 15 Jan 2022 18:58:39: 19000000 INFO @ Sat, 15 Jan 2022 18:58:42: 25000000 INFO @ Sat, 15 Jan 2022 18:58:45: 20000000 INFO @ Sat, 15 Jan 2022 18:58:48: 26000000 INFO @ Sat, 15 Jan 2022 18:58:50: 21000000 INFO @ Sat, 15 Jan 2022 18:58:54: 27000000 INFO @ Sat, 15 Jan 2022 18:58:55: 22000000 INFO @ Sat, 15 Jan 2022 18:59:00: 28000000 INFO @ Sat, 15 Jan 2022 18:59:01: 23000000 INFO @ Sat, 15 Jan 2022 18:59:04: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:59:04: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:59:04: #1 total tags in treatment: 9394143 INFO @ Sat, 15 Jan 2022 18:59:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:04: #1 tags after filtering in treatment: 4766689 INFO @ Sat, 15 Jan 2022 18:59:04: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 18:59:04: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:59:06: 24000000 INFO @ Sat, 15 Jan 2022 18:59:11: 25000000 INFO @ Sat, 15 Jan 2022 18:59:17: 26000000 INFO @ Sat, 15 Jan 2022 18:59:22: 27000000 INFO @ Sat, 15 Jan 2022 18:59:27: 28000000 INFO @ Sat, 15 Jan 2022 18:59:32: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:59:32: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:59:32: #1 total tags in treatment: 9394143 INFO @ Sat, 15 Jan 2022 18:59:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:32: #1 tags after filtering in treatment: 4766689 INFO @ Sat, 15 Jan 2022 18:59:32: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 18:59:32: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496399/SRX7496399.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling