Job ID = 14520183 SRX = SRX7496386 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10522215 spots for SRR10822997/SRR10822997.sra Written 10522215 spots for SRR10822997/SRR10822997.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:07 10522215 reads; of these: 10522215 (100.00%) were paired; of these: 5688152 (54.06%) aligned concordantly 0 times 4585629 (43.58%) aligned concordantly exactly 1 time 248434 (2.36%) aligned concordantly >1 times ---- 5688152 pairs aligned concordantly 0 times; of these: 55026 (0.97%) aligned discordantly 1 time ---- 5633126 pairs aligned 0 times concordantly or discordantly; of these: 11266252 mates make up the pairs; of these: 6323331 (56.13%) aligned 0 times 4652814 (41.30%) aligned exactly 1 time 290107 (2.58%) aligned >1 times 69.95% overall alignment rate Time searching: 00:05:07 Overall time: 00:05:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 197358 / 4888498 = 0.0404 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:42:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:42:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:42:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:42:05: 1000000 INFO @ Sat, 15 Jan 2022 18:42:09: 2000000 INFO @ Sat, 15 Jan 2022 18:42:13: 3000000 INFO @ Sat, 15 Jan 2022 18:42:17: 4000000 INFO @ Sat, 15 Jan 2022 18:42:21: 5000000 INFO @ Sat, 15 Jan 2022 18:42:26: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:42:30: 7000000 INFO @ Sat, 15 Jan 2022 18:42:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:42:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:42:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:42:34: 8000000 INFO @ Sat, 15 Jan 2022 18:42:35: 1000000 INFO @ Sat, 15 Jan 2022 18:42:38: 9000000 INFO @ Sat, 15 Jan 2022 18:42:40: 2000000 INFO @ Sat, 15 Jan 2022 18:42:43: 10000000 INFO @ Sat, 15 Jan 2022 18:42:45: 3000000 INFO @ Sat, 15 Jan 2022 18:42:47: 11000000 INFO @ Sat, 15 Jan 2022 18:42:50: 4000000 INFO @ Sat, 15 Jan 2022 18:42:51: 12000000 INFO @ Sat, 15 Jan 2022 18:42:55: 5000000 INFO @ Sat, 15 Jan 2022 18:42:56: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:43:00: 14000000 INFO @ Sat, 15 Jan 2022 18:43:00: 6000000 INFO @ Sat, 15 Jan 2022 18:43:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:43:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:43:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:43:01: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:43:01: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:43:01: #1 total tags in treatment: 4637123 INFO @ Sat, 15 Jan 2022 18:43:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:43:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:43:02: #1 tags after filtering in treatment: 2747554 INFO @ Sat, 15 Jan 2022 18:43:02: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 18:43:02: #1 finished! INFO @ Sat, 15 Jan 2022 18:43:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:43:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:43:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:43:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:43:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:43:05: 1000000 INFO @ Sat, 15 Jan 2022 18:43:05: 7000000 INFO @ Sat, 15 Jan 2022 18:43:09: 2000000 INFO @ Sat, 15 Jan 2022 18:43:10: 8000000 INFO @ Sat, 15 Jan 2022 18:43:14: 3000000 INFO @ Sat, 15 Jan 2022 18:43:15: 9000000 INFO @ Sat, 15 Jan 2022 18:43:19: 4000000 INFO @ Sat, 15 Jan 2022 18:43:20: 10000000 INFO @ Sat, 15 Jan 2022 18:43:23: 5000000 INFO @ Sat, 15 Jan 2022 18:43:25: 11000000 INFO @ Sat, 15 Jan 2022 18:43:28: 6000000 INFO @ Sat, 15 Jan 2022 18:43:29: 12000000 INFO @ Sat, 15 Jan 2022 18:43:33: 7000000 INFO @ Sat, 15 Jan 2022 18:43:34: 13000000 INFO @ Sat, 15 Jan 2022 18:43:38: 8000000 INFO @ Sat, 15 Jan 2022 18:43:39: 14000000 INFO @ Sat, 15 Jan 2022 18:43:41: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:43:41: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:43:41: #1 total tags in treatment: 4637123 INFO @ Sat, 15 Jan 2022 18:43:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:43:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:43:41: #1 tags after filtering in treatment: 2747554 INFO @ Sat, 15 Jan 2022 18:43:41: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 18:43:41: #1 finished! INFO @ Sat, 15 Jan 2022 18:43:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:43:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:43:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:43:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:43:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:43:43: 9000000 INFO @ Sat, 15 Jan 2022 18:43:48: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:43:53: 11000000 INFO @ Sat, 15 Jan 2022 18:43:58: 12000000 INFO @ Sat, 15 Jan 2022 18:44:02: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:44:07: 14000000 INFO @ Sat, 15 Jan 2022 18:44:09: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:44:09: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:44:09: #1 total tags in treatment: 4637123 INFO @ Sat, 15 Jan 2022 18:44:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:44:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:44:09: #1 tags after filtering in treatment: 2747554 INFO @ Sat, 15 Jan 2022 18:44:09: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 18:44:09: #1 finished! INFO @ Sat, 15 Jan 2022 18:44:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:44:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:44:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:44:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:44:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling