Job ID = 14520182 SRX = SRX7496385 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10829210 spots for SRR10822996/SRR10822996.sra Written 10829210 spots for SRR10822996/SRR10822996.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:08 10829210 reads; of these: 10829210 (100.00%) were paired; of these: 5904880 (54.53%) aligned concordantly 0 times 4319749 (39.89%) aligned concordantly exactly 1 time 604581 (5.58%) aligned concordantly >1 times ---- 5904880 pairs aligned concordantly 0 times; of these: 36629 (0.62%) aligned discordantly 1 time ---- 5868251 pairs aligned 0 times concordantly or discordantly; of these: 11736502 mates make up the pairs; of these: 6399912 (54.53%) aligned 0 times 4674373 (39.83%) aligned exactly 1 time 662217 (5.64%) aligned >1 times 70.45% overall alignment rate Time searching: 00:06:08 Overall time: 00:06:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 287742 / 4959221 = 0.0580 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:43:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:43:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:43:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:43:18: 1000000 INFO @ Sat, 15 Jan 2022 18:43:24: 2000000 INFO @ Sat, 15 Jan 2022 18:43:30: 3000000 INFO @ Sat, 15 Jan 2022 18:43:36: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:43:42: 5000000 INFO @ Sat, 15 Jan 2022 18:43:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:43:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:43:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:43:48: 6000000 INFO @ Sat, 15 Jan 2022 18:43:48: 1000000 INFO @ Sat, 15 Jan 2022 18:43:54: 7000000 INFO @ Sat, 15 Jan 2022 18:43:55: 2000000 INFO @ Sat, 15 Jan 2022 18:44:00: 3000000 INFO @ Sat, 15 Jan 2022 18:44:00: 8000000 INFO @ Sat, 15 Jan 2022 18:44:06: 4000000 INFO @ Sat, 15 Jan 2022 18:44:07: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:44:11: 5000000 INFO @ Sat, 15 Jan 2022 18:44:12: 10000000 INFO @ Sat, 15 Jan 2022 18:44:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:44:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:44:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:44:17: 6000000 INFO @ Sat, 15 Jan 2022 18:44:17: 11000000 INFO @ Sat, 15 Jan 2022 18:44:18: 1000000 INFO @ Sat, 15 Jan 2022 18:44:22: 12000000 INFO @ Sat, 15 Jan 2022 18:44:23: 7000000 INFO @ Sat, 15 Jan 2022 18:44:24: 2000000 INFO @ Sat, 15 Jan 2022 18:44:27: 13000000 INFO @ Sat, 15 Jan 2022 18:44:29: 8000000 INFO @ Sat, 15 Jan 2022 18:44:30: 3000000 INFO @ Sat, 15 Jan 2022 18:44:33: 14000000 INFO @ Sat, 15 Jan 2022 18:44:35: 9000000 INFO @ Sat, 15 Jan 2022 18:44:36: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:44:36: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:44:36: #1 total tags in treatment: 4636896 INFO @ Sat, 15 Jan 2022 18:44:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:44:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:44:36: #1 tags after filtering in treatment: 2914551 INFO @ Sat, 15 Jan 2022 18:44:36: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 18:44:36: #1 finished! INFO @ Sat, 15 Jan 2022 18:44:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:44:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:44:36: #2 number of paired peaks: 63 WARNING @ Sat, 15 Jan 2022 18:44:36: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:44:36: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 18:44:36: 4000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:44:41: 10000000 INFO @ Sat, 15 Jan 2022 18:44:42: 5000000 INFO @ Sat, 15 Jan 2022 18:44:47: 11000000 INFO @ Sat, 15 Jan 2022 18:44:48: 6000000 INFO @ Sat, 15 Jan 2022 18:44:53: 12000000 INFO @ Sat, 15 Jan 2022 18:44:54: 7000000 INFO @ Sat, 15 Jan 2022 18:44:59: 13000000 INFO @ Sat, 15 Jan 2022 18:45:00: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:45:04: 14000000 INFO @ Sat, 15 Jan 2022 18:45:06: 9000000 INFO @ Sat, 15 Jan 2022 18:45:08: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:45:08: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:45:08: #1 total tags in treatment: 4636896 INFO @ Sat, 15 Jan 2022 18:45:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:45:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:45:09: #1 tags after filtering in treatment: 2914551 INFO @ Sat, 15 Jan 2022 18:45:09: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 18:45:09: #1 finished! INFO @ Sat, 15 Jan 2022 18:45:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:45:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:45:09: #2 number of paired peaks: 63 WARNING @ Sat, 15 Jan 2022 18:45:09: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:45:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:45:11: 10000000 INFO @ Sat, 15 Jan 2022 18:45:17: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:45:22: 12000000 INFO @ Sat, 15 Jan 2022 18:45:28: 13000000 INFO @ Sat, 15 Jan 2022 18:45:34: 14000000 INFO @ Sat, 15 Jan 2022 18:45:37: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 18:45:37: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 18:45:37: #1 total tags in treatment: 4636896 INFO @ Sat, 15 Jan 2022 18:45:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:45:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:45:37: #1 tags after filtering in treatment: 2914551 INFO @ Sat, 15 Jan 2022 18:45:37: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 18:45:37: #1 finished! INFO @ Sat, 15 Jan 2022 18:45:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:45:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:45:38: #2 number of paired peaks: 63 WARNING @ Sat, 15 Jan 2022 18:45:38: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:45:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496385/SRX7496385.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling