Job ID = 5791654 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 42,196,887 reads read : 84,393,774 reads written : 84,393,774 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:59 42196887 reads; of these: 42196887 (100.00%) were paired; of these: 1231121 (2.92%) aligned concordantly 0 times 37000790 (87.69%) aligned concordantly exactly 1 time 3964976 (9.40%) aligned concordantly >1 times ---- 1231121 pairs aligned concordantly 0 times; of these: 105720 (8.59%) aligned discordantly 1 time ---- 1125401 pairs aligned 0 times concordantly or discordantly; of these: 2250802 mates make up the pairs; of these: 1781703 (79.16%) aligned 0 times 382702 (17.00%) aligned exactly 1 time 86397 (3.84%) aligned >1 times 97.89% overall alignment rate Time searching: 00:22:59 Overall time: 00:22:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 12824461 / 40983304 = 0.3129 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 11:12:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 11:12:31: #1 read tag files... INFO @ Wed, 22 Apr 2020 11:12:31: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 11:12:36: 1000000 INFO @ Wed, 22 Apr 2020 11:12:41: 2000000 INFO @ Wed, 22 Apr 2020 11:12:46: 3000000 INFO @ Wed, 22 Apr 2020 11:12:51: 4000000 INFO @ Wed, 22 Apr 2020 11:12:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 11:13:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 11:13:00: #1 read tag files... INFO @ Wed, 22 Apr 2020 11:13:00: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 11:13:01: 6000000 INFO @ Wed, 22 Apr 2020 11:13:05: 1000000 INFO @ Wed, 22 Apr 2020 11:13:06: 7000000 INFO @ Wed, 22 Apr 2020 11:13:11: 2000000 INFO @ Wed, 22 Apr 2020 11:13:11: 8000000 INFO @ Wed, 22 Apr 2020 11:13:16: 3000000 INFO @ Wed, 22 Apr 2020 11:13:16: 9000000 INFO @ Wed, 22 Apr 2020 11:13:21: 4000000 INFO @ Wed, 22 Apr 2020 11:13:21: 10000000 INFO @ Wed, 22 Apr 2020 11:13:26: 5000000 INFO @ Wed, 22 Apr 2020 11:13:26: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 11:13:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 11:13:30: #1 read tag files... INFO @ Wed, 22 Apr 2020 11:13:30: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 11:13:31: 6000000 INFO @ Wed, 22 Apr 2020 11:13:31: 12000000 INFO @ Wed, 22 Apr 2020 11:13:35: 1000000 INFO @ Wed, 22 Apr 2020 11:13:36: 7000000 INFO @ Wed, 22 Apr 2020 11:13:36: 13000000 INFO @ Wed, 22 Apr 2020 11:13:41: 2000000 INFO @ Wed, 22 Apr 2020 11:13:41: 8000000 INFO @ Wed, 22 Apr 2020 11:13:41: 14000000 INFO @ Wed, 22 Apr 2020 11:13:45: 3000000 INFO @ Wed, 22 Apr 2020 11:13:46: 9000000 INFO @ Wed, 22 Apr 2020 11:13:46: 15000000 INFO @ Wed, 22 Apr 2020 11:13:51: 4000000 INFO @ Wed, 22 Apr 2020 11:13:51: 10000000 INFO @ Wed, 22 Apr 2020 11:13:51: 16000000 INFO @ Wed, 22 Apr 2020 11:13:55: 5000000 INFO @ Wed, 22 Apr 2020 11:13:56: 11000000 INFO @ Wed, 22 Apr 2020 11:13:57: 17000000 INFO @ Wed, 22 Apr 2020 11:14:00: 6000000 INFO @ Wed, 22 Apr 2020 11:14:01: 12000000 INFO @ Wed, 22 Apr 2020 11:14:02: 18000000 INFO @ Wed, 22 Apr 2020 11:14:05: 7000000 INFO @ Wed, 22 Apr 2020 11:14:06: 13000000 INFO @ Wed, 22 Apr 2020 11:14:07: 19000000 INFO @ Wed, 22 Apr 2020 11:14:10: 8000000 INFO @ Wed, 22 Apr 2020 11:14:11: 14000000 INFO @ Wed, 22 Apr 2020 11:14:12: 20000000 INFO @ Wed, 22 Apr 2020 11:14:16: 9000000 INFO @ Wed, 22 Apr 2020 11:14:17: 15000000 INFO @ Wed, 22 Apr 2020 11:14:17: 21000000 INFO @ Wed, 22 Apr 2020 11:14:21: 10000000 INFO @ Wed, 22 Apr 2020 11:14:22: 16000000 INFO @ Wed, 22 Apr 2020 11:14:22: 22000000 INFO @ Wed, 22 Apr 2020 11:14:26: 11000000 INFO @ Wed, 22 Apr 2020 11:14:27: 17000000 INFO @ Wed, 22 Apr 2020 11:14:27: 23000000 INFO @ Wed, 22 Apr 2020 11:14:31: 12000000 INFO @ Wed, 22 Apr 2020 11:14:32: 18000000 INFO @ Wed, 22 Apr 2020 11:14:32: 24000000 INFO @ Wed, 22 Apr 2020 11:14:36: 13000000 INFO @ Wed, 22 Apr 2020 11:14:37: 19000000 INFO @ Wed, 22 Apr 2020 11:14:37: 25000000 INFO @ Wed, 22 Apr 2020 11:14:41: 14000000 INFO @ Wed, 22 Apr 2020 11:14:42: 20000000 INFO @ Wed, 22 Apr 2020 11:14:42: 26000000 INFO @ Wed, 22 Apr 2020 11:14:46: 15000000 INFO @ Wed, 22 Apr 2020 11:14:47: 21000000 INFO @ Wed, 22 Apr 2020 11:14:48: 27000000 INFO @ Wed, 22 Apr 2020 11:14:51: 16000000 INFO @ Wed, 22 Apr 2020 11:14:53: 22000000 INFO @ Wed, 22 Apr 2020 11:14:53: 28000000 INFO @ Wed, 22 Apr 2020 11:14:56: 17000000 INFO @ Wed, 22 Apr 2020 11:14:58: 23000000 INFO @ Wed, 22 Apr 2020 11:14:58: 29000000 INFO @ Wed, 22 Apr 2020 11:15:01: 18000000 INFO @ Wed, 22 Apr 2020 11:15:03: 24000000 INFO @ Wed, 22 Apr 2020 11:15:03: 30000000 INFO @ Wed, 22 Apr 2020 11:15:06: 19000000 INFO @ Wed, 22 Apr 2020 11:15:08: 25000000 INFO @ Wed, 22 Apr 2020 11:15:09: 31000000 INFO @ Wed, 22 Apr 2020 11:15:11: 20000000 INFO @ Wed, 22 Apr 2020 11:15:13: 26000000 INFO @ Wed, 22 Apr 2020 11:15:14: 32000000 INFO @ Wed, 22 Apr 2020 11:15:16: 21000000 INFO @ Wed, 22 Apr 2020 11:15:19: 27000000 INFO @ Wed, 22 Apr 2020 11:15:19: 33000000 INFO @ Wed, 22 Apr 2020 11:15:21: 22000000 INFO @ Wed, 22 Apr 2020 11:15:24: 28000000 INFO @ Wed, 22 Apr 2020 11:15:24: 34000000 INFO @ Wed, 22 Apr 2020 11:15:26: 23000000 INFO @ Wed, 22 Apr 2020 11:15:29: 29000000 INFO @ Wed, 22 Apr 2020 11:15:29: 35000000 INFO @ Wed, 22 Apr 2020 11:15:30: 24000000 INFO @ Wed, 22 Apr 2020 11:15:34: 25000000 INFO @ Wed, 22 Apr 2020 11:15:34: 30000000 INFO @ Wed, 22 Apr 2020 11:15:34: 36000000 INFO @ Wed, 22 Apr 2020 11:15:39: 37000000 INFO @ Wed, 22 Apr 2020 11:15:39: 26000000 INFO @ Wed, 22 Apr 2020 11:15:39: 31000000 INFO @ Wed, 22 Apr 2020 11:15:44: 38000000 INFO @ Wed, 22 Apr 2020 11:15:44: 27000000 INFO @ Wed, 22 Apr 2020 11:15:44: 32000000 INFO @ Wed, 22 Apr 2020 11:15:49: 39000000 INFO @ Wed, 22 Apr 2020 11:15:49: 33000000 INFO @ Wed, 22 Apr 2020 11:15:49: 28000000 INFO @ Wed, 22 Apr 2020 11:15:54: 40000000 INFO @ Wed, 22 Apr 2020 11:15:54: 34000000 INFO @ Wed, 22 Apr 2020 11:15:54: 29000000 INFO @ Wed, 22 Apr 2020 11:15:59: 41000000 INFO @ Wed, 22 Apr 2020 11:15:59: 35000000 INFO @ Wed, 22 Apr 2020 11:15:59: 30000000 INFO @ Wed, 22 Apr 2020 11:16:04: 42000000 INFO @ Wed, 22 Apr 2020 11:16:04: 36000000 INFO @ Wed, 22 Apr 2020 11:16:04: 31000000 INFO @ Wed, 22 Apr 2020 11:16:09: 43000000 INFO @ Wed, 22 Apr 2020 11:16:09: 32000000 INFO @ Wed, 22 Apr 2020 11:16:09: 37000000 INFO @ Wed, 22 Apr 2020 11:16:14: 33000000 INFO @ Wed, 22 Apr 2020 11:16:14: 44000000 INFO @ Wed, 22 Apr 2020 11:16:14: 38000000 INFO @ Wed, 22 Apr 2020 11:16:19: 34000000 INFO @ Wed, 22 Apr 2020 11:16:19: 39000000 INFO @ Wed, 22 Apr 2020 11:16:20: 45000000 INFO @ Wed, 22 Apr 2020 11:16:24: 35000000 INFO @ Wed, 22 Apr 2020 11:16:24: 40000000 INFO @ Wed, 22 Apr 2020 11:16:25: 46000000 INFO @ Wed, 22 Apr 2020 11:16:29: 41000000 INFO @ Wed, 22 Apr 2020 11:16:30: 47000000 INFO @ Wed, 22 Apr 2020 11:16:30: 36000000 INFO @ Wed, 22 Apr 2020 11:16:35: 42000000 INFO @ Wed, 22 Apr 2020 11:16:35: 48000000 INFO @ Wed, 22 Apr 2020 11:16:36: 37000000 INFO @ Wed, 22 Apr 2020 11:16:40: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 11:16:40: 49000000 INFO @ Wed, 22 Apr 2020 11:16:42: 38000000 INFO @ Wed, 22 Apr 2020 11:16:45: 44000000 INFO @ Wed, 22 Apr 2020 11:16:45: 50000000 INFO @ Wed, 22 Apr 2020 11:16:47: 39000000 INFO @ Wed, 22 Apr 2020 11:16:50: 45000000 INFO @ Wed, 22 Apr 2020 11:16:50: 51000000 INFO @ Wed, 22 Apr 2020 11:16:52: 40000000 INFO @ Wed, 22 Apr 2020 11:16:55: 46000000 INFO @ Wed, 22 Apr 2020 11:16:55: 52000000 BigWig に変換しました。 INFO @ Wed, 22 Apr 2020 11:16:56: 41000000 INFO @ Wed, 22 Apr 2020 11:17:00: 47000000 INFO @ Wed, 22 Apr 2020 11:17:00: 53000000 INFO @ Wed, 22 Apr 2020 11:17:01: 42000000 INFO @ Wed, 22 Apr 2020 11:17:05: 48000000 INFO @ Wed, 22 Apr 2020 11:17:06: 54000000 INFO @ Wed, 22 Apr 2020 11:17:06: 43000000 INFO @ Wed, 22 Apr 2020 11:17:10: 49000000 INFO @ Wed, 22 Apr 2020 11:17:11: 55000000 INFO @ Wed, 22 Apr 2020 11:17:11: 44000000 INFO @ Wed, 22 Apr 2020 11:17:15: 50000000 INFO @ Wed, 22 Apr 2020 11:17:16: 56000000 INFO @ Wed, 22 Apr 2020 11:17:17: 45000000 INFO @ Wed, 22 Apr 2020 11:17:20: 51000000 INFO @ Wed, 22 Apr 2020 11:17:21: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 11:17:21: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 11:17:21: #1 total tags in treatment: 28144905 INFO @ Wed, 22 Apr 2020 11:17:21: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 11:17:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 11:17:22: #1 tags after filtering in treatment: 13108234 INFO @ Wed, 22 Apr 2020 11:17:22: #1 Redundant rate of treatment: 0.53 INFO @ Wed, 22 Apr 2020 11:17:22: #1 finished! INFO @ Wed, 22 Apr 2020 11:17:22: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 11:17:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 11:17:22: 46000000 INFO @ Wed, 22 Apr 2020 11:17:22: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 11:17:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 11:17:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 11:17:25: 52000000 INFO @ Wed, 22 Apr 2020 11:17:27: 47000000 INFO @ Wed, 22 Apr 2020 11:17:30: 53000000 INFO @ Wed, 22 Apr 2020 11:17:32: 48000000 INFO @ Wed, 22 Apr 2020 11:17:35: 54000000 INFO @ Wed, 22 Apr 2020 11:17:37: 49000000 INFO @ Wed, 22 Apr 2020 11:17:40: 55000000 INFO @ Wed, 22 Apr 2020 11:17:42: 50000000 INFO @ Wed, 22 Apr 2020 11:17:45: 56000000 INFO @ Wed, 22 Apr 2020 11:17:47: 51000000 INFO @ Wed, 22 Apr 2020 11:17:50: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 11:17:50: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 11:17:50: #1 total tags in treatment: 28144905 INFO @ Wed, 22 Apr 2020 11:17:50: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 11:17:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 11:17:51: #1 tags after filtering in treatment: 13108234 INFO @ Wed, 22 Apr 2020 11:17:51: #1 Redundant rate of treatment: 0.53 INFO @ Wed, 22 Apr 2020 11:17:51: #1 finished! INFO @ Wed, 22 Apr 2020 11:17:51: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 11:17:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 11:17:51: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 11:17:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 11:17:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 11:17:52: 52000000 INFO @ Wed, 22 Apr 2020 11:17:57: 53000000 INFO @ Wed, 22 Apr 2020 11:18:01: 54000000 INFO @ Wed, 22 Apr 2020 11:18:06: 55000000 INFO @ Wed, 22 Apr 2020 11:18:11: 56000000 INFO @ Wed, 22 Apr 2020 11:18:16: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 11:18:16: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 11:18:16: #1 total tags in treatment: 28144905 INFO @ Wed, 22 Apr 2020 11:18:16: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 11:18:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 11:18:17: #1 tags after filtering in treatment: 13108234 INFO @ Wed, 22 Apr 2020 11:18:17: #1 Redundant rate of treatment: 0.53 INFO @ Wed, 22 Apr 2020 11:18:17: #1 finished! INFO @ Wed, 22 Apr 2020 11:18:17: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 11:18:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 11:18:18: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 11:18:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 11:18:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409618/SRX7409618.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling