Job ID = 5791652 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 25,655,940 reads read : 51,311,880 reads written : 51,311,880 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:29 25655940 reads; of these: 25655940 (100.00%) were paired; of these: 655333 (2.55%) aligned concordantly 0 times 22475744 (87.60%) aligned concordantly exactly 1 time 2524863 (9.84%) aligned concordantly >1 times ---- 655333 pairs aligned concordantly 0 times; of these: 39005 (5.95%) aligned discordantly 1 time ---- 616328 pairs aligned 0 times concordantly or discordantly; of these: 1232656 mates make up the pairs; of these: 954660 (77.45%) aligned 0 times 234285 (19.01%) aligned exactly 1 time 43711 (3.55%) aligned >1 times 98.14% overall alignment rate Time searching: 00:14:29 Overall time: 00:14:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3718295 / 25006667 = 0.1487 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:45:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:45:12: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:45:12: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:45:17: 1000000 INFO @ Wed, 22 Apr 2020 10:45:23: 2000000 INFO @ Wed, 22 Apr 2020 10:45:28: 3000000 INFO @ Wed, 22 Apr 2020 10:45:34: 4000000 INFO @ Wed, 22 Apr 2020 10:45:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:45:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:45:42: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:45:42: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:45:45: 6000000 INFO @ Wed, 22 Apr 2020 10:45:48: 1000000 INFO @ Wed, 22 Apr 2020 10:45:51: 7000000 INFO @ Wed, 22 Apr 2020 10:45:53: 2000000 INFO @ Wed, 22 Apr 2020 10:45:57: 8000000 INFO @ Wed, 22 Apr 2020 10:45:59: 3000000 INFO @ Wed, 22 Apr 2020 10:46:02: 9000000 INFO @ Wed, 22 Apr 2020 10:46:05: 4000000 INFO @ Wed, 22 Apr 2020 10:46:08: 10000000 INFO @ Wed, 22 Apr 2020 10:46:10: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:46:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:46:12: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:46:12: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:46:13: 11000000 INFO @ Wed, 22 Apr 2020 10:46:15: 6000000 INFO @ Wed, 22 Apr 2020 10:46:18: 1000000 INFO @ Wed, 22 Apr 2020 10:46:19: 12000000 INFO @ Wed, 22 Apr 2020 10:46:21: 7000000 INFO @ Wed, 22 Apr 2020 10:46:24: 2000000 INFO @ Wed, 22 Apr 2020 10:46:25: 13000000 INFO @ Wed, 22 Apr 2020 10:46:27: 8000000 INFO @ Wed, 22 Apr 2020 10:46:30: 3000000 INFO @ Wed, 22 Apr 2020 10:46:31: 14000000 INFO @ Wed, 22 Apr 2020 10:46:33: 9000000 INFO @ Wed, 22 Apr 2020 10:46:36: 4000000 INFO @ Wed, 22 Apr 2020 10:46:37: 15000000 INFO @ Wed, 22 Apr 2020 10:46:39: 10000000 INFO @ Wed, 22 Apr 2020 10:46:42: 5000000 INFO @ Wed, 22 Apr 2020 10:46:43: 16000000 INFO @ Wed, 22 Apr 2020 10:46:45: 11000000 INFO @ Wed, 22 Apr 2020 10:46:48: 6000000 INFO @ Wed, 22 Apr 2020 10:46:49: 17000000 INFO @ Wed, 22 Apr 2020 10:46:51: 12000000 INFO @ Wed, 22 Apr 2020 10:46:54: 7000000 INFO @ Wed, 22 Apr 2020 10:46:55: 18000000 INFO @ Wed, 22 Apr 2020 10:46:57: 13000000 INFO @ Wed, 22 Apr 2020 10:47:00: 8000000 INFO @ Wed, 22 Apr 2020 10:47:01: 19000000 INFO @ Wed, 22 Apr 2020 10:47:03: 14000000 INFO @ Wed, 22 Apr 2020 10:47:06: 9000000 INFO @ Wed, 22 Apr 2020 10:47:07: 20000000 INFO @ Wed, 22 Apr 2020 10:47:10: 15000000 INFO @ Wed, 22 Apr 2020 10:47:12: 10000000 INFO @ Wed, 22 Apr 2020 10:47:13: 21000000 INFO @ Wed, 22 Apr 2020 10:47:15: 16000000 INFO @ Wed, 22 Apr 2020 10:47:17: 11000000 INFO @ Wed, 22 Apr 2020 10:47:19: 22000000 INFO @ Wed, 22 Apr 2020 10:47:21: 17000000 INFO @ Wed, 22 Apr 2020 10:47:23: 12000000 INFO @ Wed, 22 Apr 2020 10:47:24: 23000000 INFO @ Wed, 22 Apr 2020 10:47:27: 18000000 INFO @ Wed, 22 Apr 2020 10:47:29: 13000000 INFO @ Wed, 22 Apr 2020 10:47:30: 24000000 INFO @ Wed, 22 Apr 2020 10:47:33: 19000000 INFO @ Wed, 22 Apr 2020 10:47:35: 14000000 INFO @ Wed, 22 Apr 2020 10:47:36: 25000000 INFO @ Wed, 22 Apr 2020 10:47:39: 20000000 INFO @ Wed, 22 Apr 2020 10:47:41: 15000000 INFO @ Wed, 22 Apr 2020 10:47:42: 26000000 INFO @ Wed, 22 Apr 2020 10:47:45: 21000000 INFO @ Wed, 22 Apr 2020 10:47:47: 16000000 INFO @ Wed, 22 Apr 2020 10:47:47: 27000000 INFO @ Wed, 22 Apr 2020 10:47:51: 22000000 INFO @ Wed, 22 Apr 2020 10:47:52: 17000000 INFO @ Wed, 22 Apr 2020 10:47:53: 28000000 INFO @ Wed, 22 Apr 2020 10:47:57: 23000000 INFO @ Wed, 22 Apr 2020 10:47:58: 18000000 INFO @ Wed, 22 Apr 2020 10:47:59: 29000000 INFO @ Wed, 22 Apr 2020 10:48:03: 24000000 INFO @ Wed, 22 Apr 2020 10:48:04: 19000000 INFO @ Wed, 22 Apr 2020 10:48:05: 30000000 INFO @ Wed, 22 Apr 2020 10:48:09: 25000000 INFO @ Wed, 22 Apr 2020 10:48:10: 20000000 INFO @ Wed, 22 Apr 2020 10:48:11: 31000000 INFO @ Wed, 22 Apr 2020 10:48:15: 26000000 INFO @ Wed, 22 Apr 2020 10:48:16: 21000000 INFO @ Wed, 22 Apr 2020 10:48:17: 32000000 INFO @ Wed, 22 Apr 2020 10:48:21: 27000000 INFO @ Wed, 22 Apr 2020 10:48:22: 22000000 INFO @ Wed, 22 Apr 2020 10:48:23: 33000000 INFO @ Wed, 22 Apr 2020 10:48:27: 28000000 INFO @ Wed, 22 Apr 2020 10:48:28: 23000000 INFO @ Wed, 22 Apr 2020 10:48:28: 34000000 INFO @ Wed, 22 Apr 2020 10:48:33: 29000000 INFO @ Wed, 22 Apr 2020 10:48:34: 24000000 INFO @ Wed, 22 Apr 2020 10:48:34: 35000000 INFO @ Wed, 22 Apr 2020 10:48:39: 30000000 INFO @ Wed, 22 Apr 2020 10:48:39: 36000000 INFO @ Wed, 22 Apr 2020 10:48:40: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 10:48:45: 37000000 INFO @ Wed, 22 Apr 2020 10:48:45: 31000000 INFO @ Wed, 22 Apr 2020 10:48:46: 26000000 INFO @ Wed, 22 Apr 2020 10:48:51: 38000000 INFO @ Wed, 22 Apr 2020 10:48:51: 32000000 INFO @ Wed, 22 Apr 2020 10:48:51: 27000000 INFO @ Wed, 22 Apr 2020 10:48:56: 39000000 INFO @ Wed, 22 Apr 2020 10:48:57: 33000000 INFO @ Wed, 22 Apr 2020 10:48:57: 28000000 BigWig に変換しました。 INFO @ Wed, 22 Apr 2020 10:49:02: 40000000 INFO @ Wed, 22 Apr 2020 10:49:03: 34000000 INFO @ Wed, 22 Apr 2020 10:49:03: 29000000 INFO @ Wed, 22 Apr 2020 10:49:08: 41000000 INFO @ Wed, 22 Apr 2020 10:49:09: 35000000 INFO @ Wed, 22 Apr 2020 10:49:10: 30000000 INFO @ Wed, 22 Apr 2020 10:49:14: 42000000 INFO @ Wed, 22 Apr 2020 10:49:15: 36000000 INFO @ Wed, 22 Apr 2020 10:49:16: 31000000 INFO @ Wed, 22 Apr 2020 10:49:19: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 10:49:19: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 10:49:19: #1 total tags in treatment: 21283234 INFO @ Wed, 22 Apr 2020 10:49:19: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:49:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:49:20: #1 tags after filtering in treatment: 11501031 INFO @ Wed, 22 Apr 2020 10:49:20: #1 Redundant rate of treatment: 0.46 INFO @ Wed, 22 Apr 2020 10:49:20: #1 finished! INFO @ Wed, 22 Apr 2020 10:49:20: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:49:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:49:20: 37000000 INFO @ Wed, 22 Apr 2020 10:49:21: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:49:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:49:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:49:21: 32000000 INFO @ Wed, 22 Apr 2020 10:49:26: 38000000 INFO @ Wed, 22 Apr 2020 10:49:27: 33000000 INFO @ Wed, 22 Apr 2020 10:49:32: 39000000 INFO @ Wed, 22 Apr 2020 10:49:33: 34000000 INFO @ Wed, 22 Apr 2020 10:49:38: 40000000 INFO @ Wed, 22 Apr 2020 10:49:39: 35000000 INFO @ Wed, 22 Apr 2020 10:49:44: 41000000 INFO @ Wed, 22 Apr 2020 10:49:44: 36000000 INFO @ Wed, 22 Apr 2020 10:49:50: 42000000 INFO @ Wed, 22 Apr 2020 10:49:50: 37000000 INFO @ Wed, 22 Apr 2020 10:49:55: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 10:49:55: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 10:49:55: #1 total tags in treatment: 21283234 INFO @ Wed, 22 Apr 2020 10:49:55: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:49:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:49:55: 38000000 INFO @ Wed, 22 Apr 2020 10:49:56: #1 tags after filtering in treatment: 11501031 INFO @ Wed, 22 Apr 2020 10:49:56: #1 Redundant rate of treatment: 0.46 INFO @ Wed, 22 Apr 2020 10:49:56: #1 finished! INFO @ Wed, 22 Apr 2020 10:49:56: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:49:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:49:56: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:49:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:49:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:50:01: 39000000 INFO @ Wed, 22 Apr 2020 10:50:06: 40000000 INFO @ Wed, 22 Apr 2020 10:50:12: 41000000 INFO @ Wed, 22 Apr 2020 10:50:18: 42000000 INFO @ Wed, 22 Apr 2020 10:50:23: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 10:50:23: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 10:50:23: #1 total tags in treatment: 21283234 INFO @ Wed, 22 Apr 2020 10:50:23: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:50:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:50:23: #1 tags after filtering in treatment: 11501031 INFO @ Wed, 22 Apr 2020 10:50:23: #1 Redundant rate of treatment: 0.46 INFO @ Wed, 22 Apr 2020 10:50:23: #1 finished! INFO @ Wed, 22 Apr 2020 10:50:23: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:50:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:50:24: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:50:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:50:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409616/SRX7409616.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling