Job ID = 5791649 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 15,491,035 reads read : 30,982,070 reads written : 30,982,070 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:04 15491035 reads; of these: 15491035 (100.00%) were paired; of these: 362948 (2.34%) aligned concordantly 0 times 13718701 (88.56%) aligned concordantly exactly 1 time 1409386 (9.10%) aligned concordantly >1 times ---- 362948 pairs aligned concordantly 0 times; of these: 11155 (3.07%) aligned discordantly 1 time ---- 351793 pairs aligned 0 times concordantly or discordantly; of these: 703586 mates make up the pairs; of these: 565254 (80.34%) aligned 0 times 118124 (16.79%) aligned exactly 1 time 20208 (2.87%) aligned >1 times 98.18% overall alignment rate Time searching: 00:08:04 Overall time: 00:08:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2646322 / 15132820 = 0.1749 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:27:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:27:59: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:27:59: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:28:04: 1000000 INFO @ Wed, 22 Apr 2020 10:28:09: 2000000 INFO @ Wed, 22 Apr 2020 10:28:13: 3000000 INFO @ Wed, 22 Apr 2020 10:28:19: 4000000 INFO @ Wed, 22 Apr 2020 10:28:24: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:28:29: 6000000 INFO @ Wed, 22 Apr 2020 10:28:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:28:30: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:28:30: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:28:34: 7000000 INFO @ Wed, 22 Apr 2020 10:28:35: 1000000 INFO @ Wed, 22 Apr 2020 10:28:39: 8000000 INFO @ Wed, 22 Apr 2020 10:28:40: 2000000 INFO @ Wed, 22 Apr 2020 10:28:45: 9000000 INFO @ Wed, 22 Apr 2020 10:28:46: 3000000 INFO @ Wed, 22 Apr 2020 10:28:50: 10000000 INFO @ Wed, 22 Apr 2020 10:28:51: 4000000 INFO @ Wed, 22 Apr 2020 10:28:56: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:28:57: 5000000 INFO @ Wed, 22 Apr 2020 10:28:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:28:58: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:28:58: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:29:01: 12000000 INFO @ Wed, 22 Apr 2020 10:29:02: 6000000 INFO @ Wed, 22 Apr 2020 10:29:04: 1000000 INFO @ Wed, 22 Apr 2020 10:29:07: 13000000 INFO @ Wed, 22 Apr 2020 10:29:08: 7000000 INFO @ Wed, 22 Apr 2020 10:29:11: 2000000 INFO @ Wed, 22 Apr 2020 10:29:13: 14000000 INFO @ Wed, 22 Apr 2020 10:29:14: 8000000 INFO @ Wed, 22 Apr 2020 10:29:18: 3000000 INFO @ Wed, 22 Apr 2020 10:29:19: 15000000 INFO @ Wed, 22 Apr 2020 10:29:20: 9000000 INFO @ Wed, 22 Apr 2020 10:29:24: 4000000 INFO @ Wed, 22 Apr 2020 10:29:25: 16000000 INFO @ Wed, 22 Apr 2020 10:29:26: 10000000 INFO @ Wed, 22 Apr 2020 10:29:31: 5000000 INFO @ Wed, 22 Apr 2020 10:29:31: 17000000 INFO @ Wed, 22 Apr 2020 10:29:32: 11000000 INFO @ Wed, 22 Apr 2020 10:29:37: 18000000 INFO @ Wed, 22 Apr 2020 10:29:37: 6000000 INFO @ Wed, 22 Apr 2020 10:29:38: 12000000 INFO @ Wed, 22 Apr 2020 10:29:43: 19000000 INFO @ Wed, 22 Apr 2020 10:29:44: 7000000 INFO @ Wed, 22 Apr 2020 10:29:44: 13000000 INFO @ Wed, 22 Apr 2020 10:29:49: 20000000 INFO @ Wed, 22 Apr 2020 10:29:50: 8000000 INFO @ Wed, 22 Apr 2020 10:29:50: 14000000 INFO @ Wed, 22 Apr 2020 10:29:55: 21000000 INFO @ Wed, 22 Apr 2020 10:29:56: 15000000 INFO @ Wed, 22 Apr 2020 10:29:57: 9000000 INFO @ Wed, 22 Apr 2020 10:30:01: 22000000 INFO @ Wed, 22 Apr 2020 10:30:02: 16000000 INFO @ Wed, 22 Apr 2020 10:30:03: 10000000 INFO @ Wed, 22 Apr 2020 10:30:07: 23000000 INFO @ Wed, 22 Apr 2020 10:30:08: 17000000 INFO @ Wed, 22 Apr 2020 10:30:10: 11000000 INFO @ Wed, 22 Apr 2020 10:30:13: 24000000 INFO @ Wed, 22 Apr 2020 10:30:14: 18000000 INFO @ Wed, 22 Apr 2020 10:30:16: 12000000 INFO @ Wed, 22 Apr 2020 10:30:19: 25000000 INFO @ Wed, 22 Apr 2020 10:30:20: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 10:30:20: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 10:30:20: #1 total tags in treatment: 12482544 INFO @ Wed, 22 Apr 2020 10:30:20: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:30:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:30:20: #1 tags after filtering in treatment: 7610937 INFO @ Wed, 22 Apr 2020 10:30:20: #1 Redundant rate of treatment: 0.39 INFO @ Wed, 22 Apr 2020 10:30:20: #1 finished! INFO @ Wed, 22 Apr 2020 10:30:20: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:30:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:30:20: 19000000 INFO @ Wed, 22 Apr 2020 10:30:20: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:30:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:30:20: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 10:30:23: 13000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:30:26: 20000000 INFO @ Wed, 22 Apr 2020 10:30:29: 14000000 INFO @ Wed, 22 Apr 2020 10:30:32: 21000000 INFO @ Wed, 22 Apr 2020 10:30:36: 15000000 INFO @ Wed, 22 Apr 2020 10:30:37: 22000000 INFO @ Wed, 22 Apr 2020 10:30:42: 16000000 INFO @ Wed, 22 Apr 2020 10:30:43: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 10:30:49: 24000000 INFO @ Wed, 22 Apr 2020 10:30:49: 17000000 INFO @ Wed, 22 Apr 2020 10:30:54: 25000000 INFO @ Wed, 22 Apr 2020 10:30:55: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 10:30:55: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 10:30:55: #1 total tags in treatment: 12482544 INFO @ Wed, 22 Apr 2020 10:30:55: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:30:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:30:55: #1 tags after filtering in treatment: 7610937 INFO @ Wed, 22 Apr 2020 10:30:55: #1 Redundant rate of treatment: 0.39 INFO @ Wed, 22 Apr 2020 10:30:55: #1 finished! INFO @ Wed, 22 Apr 2020 10:30:55: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:30:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:30:55: 18000000 INFO @ Wed, 22 Apr 2020 10:30:56: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:30:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:30:56: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:31:02: 19000000 INFO @ Wed, 22 Apr 2020 10:31:08: 20000000 INFO @ Wed, 22 Apr 2020 10:31:14: 21000000 INFO @ Wed, 22 Apr 2020 10:31:20: 22000000 INFO @ Wed, 22 Apr 2020 10:31:26: 23000000 INFO @ Wed, 22 Apr 2020 10:31:32: 24000000 INFO @ Wed, 22 Apr 2020 10:31:38: 25000000 INFO @ Wed, 22 Apr 2020 10:31:38: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 10:31:38: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 10:31:38: #1 total tags in treatment: 12482544 INFO @ Wed, 22 Apr 2020 10:31:38: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:31:39: #1 tags after filtering in treatment: 7610937 INFO @ Wed, 22 Apr 2020 10:31:39: #1 Redundant rate of treatment: 0.39 INFO @ Wed, 22 Apr 2020 10:31:39: #1 finished! INFO @ Wed, 22 Apr 2020 10:31:39: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:31:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:31:39: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:31:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:31:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409613/SRX7409613.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling