Job ID = 5791313 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 28,188,694 reads read : 56,377,388 reads written : 56,377,388 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:26 28188694 reads; of these: 28188694 (100.00%) were paired; of these: 433595 (1.54%) aligned concordantly 0 times 23624927 (83.81%) aligned concordantly exactly 1 time 4130172 (14.65%) aligned concordantly >1 times ---- 433595 pairs aligned concordantly 0 times; of these: 58676 (13.53%) aligned discordantly 1 time ---- 374919 pairs aligned 0 times concordantly or discordantly; of these: 749838 mates make up the pairs; of these: 492216 (65.64%) aligned 0 times 193540 (25.81%) aligned exactly 1 time 64082 (8.55%) aligned >1 times 99.13% overall alignment rate Time searching: 00:15:26 Overall time: 00:15:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 6398326 / 27763741 = 0.2305 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:06:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:06:22: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:06:22: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:06:29: 1000000 INFO @ Wed, 22 Apr 2020 10:06:35: 2000000 INFO @ Wed, 22 Apr 2020 10:06:42: 3000000 INFO @ Wed, 22 Apr 2020 10:06:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:06:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:06:52: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:06:52: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:06:55: 5000000 INFO @ Wed, 22 Apr 2020 10:06:59: 1000000 INFO @ Wed, 22 Apr 2020 10:07:03: 6000000 INFO @ Wed, 22 Apr 2020 10:07:05: 2000000 INFO @ Wed, 22 Apr 2020 10:07:10: 7000000 INFO @ Wed, 22 Apr 2020 10:07:11: 3000000 INFO @ Wed, 22 Apr 2020 10:07:17: 8000000 INFO @ Wed, 22 Apr 2020 10:07:17: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:07:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:07:22: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:07:22: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:07:23: 5000000 INFO @ Wed, 22 Apr 2020 10:07:24: 9000000 INFO @ Wed, 22 Apr 2020 10:07:29: 1000000 INFO @ Wed, 22 Apr 2020 10:07:30: 6000000 INFO @ Wed, 22 Apr 2020 10:07:31: 10000000 INFO @ Wed, 22 Apr 2020 10:07:35: 2000000 INFO @ Wed, 22 Apr 2020 10:07:36: 7000000 INFO @ Wed, 22 Apr 2020 10:07:38: 11000000 INFO @ Wed, 22 Apr 2020 10:07:42: 3000000 INFO @ Wed, 22 Apr 2020 10:07:43: 8000000 INFO @ Wed, 22 Apr 2020 10:07:44: 12000000 INFO @ Wed, 22 Apr 2020 10:07:48: 4000000 INFO @ Wed, 22 Apr 2020 10:07:49: 9000000 INFO @ Wed, 22 Apr 2020 10:07:51: 13000000 INFO @ Wed, 22 Apr 2020 10:07:55: 5000000 INFO @ Wed, 22 Apr 2020 10:07:55: 10000000 INFO @ Wed, 22 Apr 2020 10:07:58: 14000000 INFO @ Wed, 22 Apr 2020 10:08:01: 6000000 INFO @ Wed, 22 Apr 2020 10:08:02: 11000000 INFO @ Wed, 22 Apr 2020 10:08:05: 15000000 INFO @ Wed, 22 Apr 2020 10:08:07: 7000000 INFO @ Wed, 22 Apr 2020 10:08:08: 12000000 INFO @ Wed, 22 Apr 2020 10:08:12: 16000000 INFO @ Wed, 22 Apr 2020 10:08:14: 8000000 INFO @ Wed, 22 Apr 2020 10:08:15: 13000000 INFO @ Wed, 22 Apr 2020 10:08:19: 17000000 INFO @ Wed, 22 Apr 2020 10:08:20: 9000000 INFO @ Wed, 22 Apr 2020 10:08:21: 14000000 INFO @ Wed, 22 Apr 2020 10:08:26: 18000000 INFO @ Wed, 22 Apr 2020 10:08:27: 10000000 INFO @ Wed, 22 Apr 2020 10:08:27: 15000000 INFO @ Wed, 22 Apr 2020 10:08:33: 19000000 INFO @ Wed, 22 Apr 2020 10:08:33: 11000000 INFO @ Wed, 22 Apr 2020 10:08:34: 16000000 INFO @ Wed, 22 Apr 2020 10:08:39: 12000000 INFO @ Wed, 22 Apr 2020 10:08:40: 20000000 INFO @ Wed, 22 Apr 2020 10:08:40: 17000000 INFO @ Wed, 22 Apr 2020 10:08:46: 13000000 INFO @ Wed, 22 Apr 2020 10:08:46: 18000000 INFO @ Wed, 22 Apr 2020 10:08:47: 21000000 INFO @ Wed, 22 Apr 2020 10:08:52: 14000000 INFO @ Wed, 22 Apr 2020 10:08:53: 19000000 INFO @ Wed, 22 Apr 2020 10:08:54: 22000000 INFO @ Wed, 22 Apr 2020 10:08:59: 15000000 INFO @ Wed, 22 Apr 2020 10:08:59: 20000000 INFO @ Wed, 22 Apr 2020 10:09:00: 23000000 INFO @ Wed, 22 Apr 2020 10:09:05: 16000000 INFO @ Wed, 22 Apr 2020 10:09:05: 21000000 INFO @ Wed, 22 Apr 2020 10:09:07: 24000000 INFO @ Wed, 22 Apr 2020 10:09:11: 17000000 INFO @ Wed, 22 Apr 2020 10:09:12: 22000000 INFO @ Wed, 22 Apr 2020 10:09:14: 25000000 INFO @ Wed, 22 Apr 2020 10:09:18: 18000000 INFO @ Wed, 22 Apr 2020 10:09:18: 23000000 INFO @ Wed, 22 Apr 2020 10:09:21: 26000000 INFO @ Wed, 22 Apr 2020 10:09:24: 19000000 INFO @ Wed, 22 Apr 2020 10:09:24: 24000000 INFO @ Wed, 22 Apr 2020 10:09:28: 27000000 INFO @ Wed, 22 Apr 2020 10:09:30: 20000000 INFO @ Wed, 22 Apr 2020 10:09:31: 25000000 INFO @ Wed, 22 Apr 2020 10:09:35: 28000000 INFO @ Wed, 22 Apr 2020 10:09:36: 21000000 INFO @ Wed, 22 Apr 2020 10:09:37: 26000000 INFO @ Wed, 22 Apr 2020 10:09:42: 29000000 INFO @ Wed, 22 Apr 2020 10:09:43: 22000000 INFO @ Wed, 22 Apr 2020 10:09:43: 27000000 INFO @ Wed, 22 Apr 2020 10:09:49: 30000000 INFO @ Wed, 22 Apr 2020 10:09:49: 23000000 INFO @ Wed, 22 Apr 2020 10:09:50: 28000000 INFO @ Wed, 22 Apr 2020 10:09:55: 24000000 INFO @ Wed, 22 Apr 2020 10:09:56: 31000000 INFO @ Wed, 22 Apr 2020 10:09:56: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 10:10:02: 25000000 INFO @ Wed, 22 Apr 2020 10:10:02: 32000000 INFO @ Wed, 22 Apr 2020 10:10:02: 30000000 INFO @ Wed, 22 Apr 2020 10:10:08: 26000000 INFO @ Wed, 22 Apr 2020 10:10:09: 31000000 INFO @ Wed, 22 Apr 2020 10:10:09: 33000000 INFO @ Wed, 22 Apr 2020 10:10:15: 27000000 BigWig に変換しました。 INFO @ Wed, 22 Apr 2020 10:10:15: 32000000 INFO @ Wed, 22 Apr 2020 10:10:16: 34000000 INFO @ Wed, 22 Apr 2020 10:10:21: 28000000 INFO @ Wed, 22 Apr 2020 10:10:22: 33000000 INFO @ Wed, 22 Apr 2020 10:10:23: 35000000 INFO @ Wed, 22 Apr 2020 10:10:27: 29000000 INFO @ Wed, 22 Apr 2020 10:10:28: 34000000 INFO @ Wed, 22 Apr 2020 10:10:30: 36000000 INFO @ Wed, 22 Apr 2020 10:10:34: 30000000 INFO @ Wed, 22 Apr 2020 10:10:34: 35000000 INFO @ Wed, 22 Apr 2020 10:10:37: 37000000 INFO @ Wed, 22 Apr 2020 10:10:40: 31000000 INFO @ Wed, 22 Apr 2020 10:10:41: 36000000 INFO @ Wed, 22 Apr 2020 10:10:43: 38000000 INFO @ Wed, 22 Apr 2020 10:10:47: 32000000 INFO @ Wed, 22 Apr 2020 10:10:47: 37000000 INFO @ Wed, 22 Apr 2020 10:10:50: 39000000 INFO @ Wed, 22 Apr 2020 10:10:53: 33000000 INFO @ Wed, 22 Apr 2020 10:10:54: 38000000 INFO @ Wed, 22 Apr 2020 10:10:57: 40000000 INFO @ Wed, 22 Apr 2020 10:11:00: 34000000 INFO @ Wed, 22 Apr 2020 10:11:00: 39000000 INFO @ Wed, 22 Apr 2020 10:11:04: 41000000 INFO @ Wed, 22 Apr 2020 10:11:06: 35000000 INFO @ Wed, 22 Apr 2020 10:11:06: 40000000 INFO @ Wed, 22 Apr 2020 10:11:11: 42000000 INFO @ Wed, 22 Apr 2020 10:11:12: 36000000 INFO @ Wed, 22 Apr 2020 10:11:12: 41000000 INFO @ Wed, 22 Apr 2020 10:11:17: 43000000 INFO @ Wed, 22 Apr 2020 10:11:18: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 10:11:18: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 10:11:18: #1 total tags in treatment: 21358439 INFO @ Wed, 22 Apr 2020 10:11:18: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:11:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:11:18: 37000000 INFO @ Wed, 22 Apr 2020 10:11:18: #1 tags after filtering in treatment: 12816990 INFO @ Wed, 22 Apr 2020 10:11:18: #1 Redundant rate of treatment: 0.40 INFO @ Wed, 22 Apr 2020 10:11:18: #1 finished! INFO @ Wed, 22 Apr 2020 10:11:18: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:11:18: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:11:19: 42000000 INFO @ Wed, 22 Apr 2020 10:11:19: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:11:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:11:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:11:24: 38000000 INFO @ Wed, 22 Apr 2020 10:11:24: 43000000 INFO @ Wed, 22 Apr 2020 10:11:25: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 10:11:25: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 10:11:25: #1 total tags in treatment: 21358439 INFO @ Wed, 22 Apr 2020 10:11:25: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:11:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:11:25: #1 tags after filtering in treatment: 12816990 INFO @ Wed, 22 Apr 2020 10:11:25: #1 Redundant rate of treatment: 0.40 INFO @ Wed, 22 Apr 2020 10:11:25: #1 finished! INFO @ Wed, 22 Apr 2020 10:11:25: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:11:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:11:26: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:11:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:11:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:11:29: 39000000 INFO @ Wed, 22 Apr 2020 10:11:35: 40000000 INFO @ Wed, 22 Apr 2020 10:11:40: 41000000 INFO @ Wed, 22 Apr 2020 10:11:46: 42000000 INFO @ Wed, 22 Apr 2020 10:11:51: 43000000 INFO @ Wed, 22 Apr 2020 10:11:51: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 10:11:51: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 10:11:51: #1 total tags in treatment: 21358439 INFO @ Wed, 22 Apr 2020 10:11:51: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:11:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:11:52: #1 tags after filtering in treatment: 12816990 INFO @ Wed, 22 Apr 2020 10:11:52: #1 Redundant rate of treatment: 0.40 INFO @ Wed, 22 Apr 2020 10:11:52: #1 finished! INFO @ Wed, 22 Apr 2020 10:11:52: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:11:52: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:11:53: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:11:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:11:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7409567/SRX7409567.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling