Job ID = 7108055 SRX = SRX7217648 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30626183 spots for SRR10533725/SRR10533725.sra Written 30626183 spots for SRR10533725/SRR10533725.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:06 30626183 reads; of these: 30626183 (100.00%) were paired; of these: 14986564 (48.93%) aligned concordantly 0 times 13112110 (42.81%) aligned concordantly exactly 1 time 2527509 (8.25%) aligned concordantly >1 times ---- 14986564 pairs aligned concordantly 0 times; of these: 3478193 (23.21%) aligned discordantly 1 time ---- 11508371 pairs aligned 0 times concordantly or discordantly; of these: 23016742 mates make up the pairs; of these: 21253474 (92.34%) aligned 0 times 427618 (1.86%) aligned exactly 1 time 1335650 (5.80%) aligned >1 times 65.30% overall alignment rate Time searching: 00:31:06 Overall time: 00:31:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5191354 / 18967898 = 0.2737 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:06:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:06:37: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:06:37: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:06:46: 1000000 INFO @ Wed, 22 Jul 2020 14:06:56: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:07:06: 3000000 INFO @ Wed, 22 Jul 2020 14:07:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:07:07: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:07:07: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:07:17: 4000000 INFO @ Wed, 22 Jul 2020 14:07:19: 1000000 INFO @ Wed, 22 Jul 2020 14:07:28: 5000000 INFO @ Wed, 22 Jul 2020 14:07:32: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:07:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:07:37: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:07:37: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:07:39: 6000000 INFO @ Wed, 22 Jul 2020 14:07:44: 3000000 INFO @ Wed, 22 Jul 2020 14:07:49: 1000000 INFO @ Wed, 22 Jul 2020 14:07:51: 7000000 INFO @ Wed, 22 Jul 2020 14:07:57: 4000000 INFO @ Wed, 22 Jul 2020 14:08:02: 2000000 INFO @ Wed, 22 Jul 2020 14:08:02: 8000000 INFO @ Wed, 22 Jul 2020 14:08:10: 5000000 INFO @ Wed, 22 Jul 2020 14:08:14: 9000000 INFO @ Wed, 22 Jul 2020 14:08:14: 3000000 INFO @ Wed, 22 Jul 2020 14:08:24: 6000000 INFO @ Wed, 22 Jul 2020 14:08:26: 10000000 INFO @ Wed, 22 Jul 2020 14:08:27: 4000000 INFO @ Wed, 22 Jul 2020 14:08:37: 7000000 INFO @ Wed, 22 Jul 2020 14:08:38: 11000000 INFO @ Wed, 22 Jul 2020 14:08:41: 5000000 INFO @ Wed, 22 Jul 2020 14:08:49: 12000000 INFO @ Wed, 22 Jul 2020 14:08:50: 8000000 INFO @ Wed, 22 Jul 2020 14:08:54: 6000000 INFO @ Wed, 22 Jul 2020 14:09:01: 13000000 INFO @ Wed, 22 Jul 2020 14:09:04: 9000000 INFO @ Wed, 22 Jul 2020 14:09:06: 7000000 INFO @ Wed, 22 Jul 2020 14:09:13: 14000000 INFO @ Wed, 22 Jul 2020 14:09:17: 10000000 INFO @ Wed, 22 Jul 2020 14:09:19: 8000000 INFO @ Wed, 22 Jul 2020 14:09:24: 15000000 INFO @ Wed, 22 Jul 2020 14:09:30: 11000000 INFO @ Wed, 22 Jul 2020 14:09:32: 9000000 INFO @ Wed, 22 Jul 2020 14:09:36: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:09:43: 12000000 INFO @ Wed, 22 Jul 2020 14:09:44: 10000000 INFO @ Wed, 22 Jul 2020 14:09:48: 17000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:09:57: 13000000 INFO @ Wed, 22 Jul 2020 14:09:57: 11000000 INFO @ Wed, 22 Jul 2020 14:09:59: 18000000 INFO @ Wed, 22 Jul 2020 14:10:10: 12000000 INFO @ Wed, 22 Jul 2020 14:10:10: 14000000 INFO @ Wed, 22 Jul 2020 14:10:11: 19000000 INFO @ Wed, 22 Jul 2020 14:10:22: 13000000 INFO @ Wed, 22 Jul 2020 14:10:23: 20000000 INFO @ Wed, 22 Jul 2020 14:10:23: 15000000 INFO @ Wed, 22 Jul 2020 14:10:34: 21000000 INFO @ Wed, 22 Jul 2020 14:10:35: 14000000 INFO @ Wed, 22 Jul 2020 14:10:36: 16000000 INFO @ Wed, 22 Jul 2020 14:10:46: 22000000 INFO @ Wed, 22 Jul 2020 14:10:48: 15000000 INFO @ Wed, 22 Jul 2020 14:10:50: 17000000 INFO @ Wed, 22 Jul 2020 14:10:58: 23000000 INFO @ Wed, 22 Jul 2020 14:11:01: 16000000 INFO @ Wed, 22 Jul 2020 14:11:03: 18000000 INFO @ Wed, 22 Jul 2020 14:11:10: 24000000 INFO @ Wed, 22 Jul 2020 14:11:13: 17000000 INFO @ Wed, 22 Jul 2020 14:11:16: 19000000 INFO @ Wed, 22 Jul 2020 14:11:22: 25000000 INFO @ Wed, 22 Jul 2020 14:11:26: 18000000 INFO @ Wed, 22 Jul 2020 14:11:30: 20000000 INFO @ Wed, 22 Jul 2020 14:11:33: 26000000 INFO @ Wed, 22 Jul 2020 14:11:39: 19000000 INFO @ Wed, 22 Jul 2020 14:11:43: 21000000 INFO @ Wed, 22 Jul 2020 14:11:45: 27000000 INFO @ Wed, 22 Jul 2020 14:11:52: 20000000 INFO @ Wed, 22 Jul 2020 14:11:57: 28000000 INFO @ Wed, 22 Jul 2020 14:11:57: 22000000 INFO @ Wed, 22 Jul 2020 14:12:05: 21000000 INFO @ Wed, 22 Jul 2020 14:12:09: 29000000 INFO @ Wed, 22 Jul 2020 14:12:11: 23000000 INFO @ Wed, 22 Jul 2020 14:12:16: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:12:16: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:12:16: #1 total tags in treatment: 11130205 INFO @ Wed, 22 Jul 2020 14:12:16: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:12:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:12:16: #1 tags after filtering in treatment: 7905791 INFO @ Wed, 22 Jul 2020 14:12:16: #1 Redundant rate of treatment: 0.29 INFO @ Wed, 22 Jul 2020 14:12:16: #1 finished! INFO @ Wed, 22 Jul 2020 14:12:16: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:12:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:12:16: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:12:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:12:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:12:18: 22000000 INFO @ Wed, 22 Jul 2020 14:12:24: 24000000 INFO @ Wed, 22 Jul 2020 14:12:30: 23000000 INFO @ Wed, 22 Jul 2020 14:12:37: 25000000 INFO @ Wed, 22 Jul 2020 14:12:43: 24000000 INFO @ Wed, 22 Jul 2020 14:12:50: 26000000 INFO @ Wed, 22 Jul 2020 14:12:55: 25000000 INFO @ Wed, 22 Jul 2020 14:13:04: 27000000 INFO @ Wed, 22 Jul 2020 14:13:07: 26000000 INFO @ Wed, 22 Jul 2020 14:13:17: 28000000 INFO @ Wed, 22 Jul 2020 14:13:20: 27000000 INFO @ Wed, 22 Jul 2020 14:13:30: 29000000 INFO @ Wed, 22 Jul 2020 14:13:32: 28000000 INFO @ Wed, 22 Jul 2020 14:13:37: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:13:37: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:13:37: #1 total tags in treatment: 11130205 INFO @ Wed, 22 Jul 2020 14:13:37: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:13:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:13:37: #1 tags after filtering in treatment: 7905791 INFO @ Wed, 22 Jul 2020 14:13:37: #1 Redundant rate of treatment: 0.29 INFO @ Wed, 22 Jul 2020 14:13:37: #1 finished! INFO @ Wed, 22 Jul 2020 14:13:37: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:13:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:13:38: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:13:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:13:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:13:44: 29000000 INFO @ Wed, 22 Jul 2020 14:13:50: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:13:50: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:13:50: #1 total tags in treatment: 11130205 INFO @ Wed, 22 Jul 2020 14:13:50: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:13:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:13:50: #1 tags after filtering in treatment: 7905791 INFO @ Wed, 22 Jul 2020 14:13:50: #1 Redundant rate of treatment: 0.29 INFO @ Wed, 22 Jul 2020 14:13:50: #1 finished! INFO @ Wed, 22 Jul 2020 14:13:50: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:13:50: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:13:51: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:13:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:13:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217648/SRX7217648.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling