Job ID = 7108028 SRX = SRX7217647 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30925622 spots for SRR10533724/SRR10533724.sra Written 30925622 spots for SRR10533724/SRR10533724.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:44:52 30925622 reads; of these: 30925622 (100.00%) were paired; of these: 8585019 (27.76%) aligned concordantly 0 times 18455701 (59.68%) aligned concordantly exactly 1 time 3884902 (12.56%) aligned concordantly >1 times ---- 8585019 pairs aligned concordantly 0 times; of these: 5427749 (63.22%) aligned discordantly 1 time ---- 3157270 pairs aligned 0 times concordantly or discordantly; of these: 6314540 mates make up the pairs; of these: 2598104 (41.14%) aligned 0 times 986489 (15.62%) aligned exactly 1 time 2729947 (43.23%) aligned >1 times 95.80% overall alignment rate Time searching: 00:44:52 Overall time: 00:44:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5559071 / 27041560 = 0.2056 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:25:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:25:25: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:25:25: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:25:33: 1000000 INFO @ Wed, 22 Jul 2020 14:25:41: 2000000 INFO @ Wed, 22 Jul 2020 14:25:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:25:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:25:55: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:25:55: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:25:56: 4000000 INFO @ Wed, 22 Jul 2020 14:26:04: 1000000 INFO @ Wed, 22 Jul 2020 14:26:04: 5000000 INFO @ Wed, 22 Jul 2020 14:26:13: 2000000 INFO @ Wed, 22 Jul 2020 14:26:13: 6000000 INFO @ Wed, 22 Jul 2020 14:26:21: 3000000 INFO @ Wed, 22 Jul 2020 14:26:22: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:26:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:26:27: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:26:27: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:26:31: 4000000 INFO @ Wed, 22 Jul 2020 14:26:31: 8000000 INFO @ Wed, 22 Jul 2020 14:26:38: 1000000 INFO @ Wed, 22 Jul 2020 14:26:41: 9000000 INFO @ Wed, 22 Jul 2020 14:26:42: 5000000 INFO @ Wed, 22 Jul 2020 14:26:49: 2000000 INFO @ Wed, 22 Jul 2020 14:26:51: 10000000 INFO @ Wed, 22 Jul 2020 14:26:54: 6000000 INFO @ Wed, 22 Jul 2020 14:27:00: 11000000 INFO @ Wed, 22 Jul 2020 14:27:01: 3000000 INFO @ Wed, 22 Jul 2020 14:27:05: 7000000 INFO @ Wed, 22 Jul 2020 14:27:10: 12000000 INFO @ Wed, 22 Jul 2020 14:27:12: 4000000 INFO @ Wed, 22 Jul 2020 14:27:17: 8000000 INFO @ Wed, 22 Jul 2020 14:27:20: 13000000 INFO @ Wed, 22 Jul 2020 14:27:23: 5000000 INFO @ Wed, 22 Jul 2020 14:27:28: 9000000 INFO @ Wed, 22 Jul 2020 14:27:29: 14000000 INFO @ Wed, 22 Jul 2020 14:27:35: 6000000 INFO @ Wed, 22 Jul 2020 14:27:39: 15000000 INFO @ Wed, 22 Jul 2020 14:27:39: 10000000 INFO @ Wed, 22 Jul 2020 14:27:46: 7000000 INFO @ Wed, 22 Jul 2020 14:27:49: 16000000 INFO @ Wed, 22 Jul 2020 14:27:51: 11000000 INFO @ Wed, 22 Jul 2020 14:27:58: 8000000 INFO @ Wed, 22 Jul 2020 14:27:59: 17000000 INFO @ Wed, 22 Jul 2020 14:28:02: 12000000 INFO @ Wed, 22 Jul 2020 14:28:09: 18000000 INFO @ Wed, 22 Jul 2020 14:28:10: 9000000 INFO @ Wed, 22 Jul 2020 14:28:13: 13000000 INFO @ Wed, 22 Jul 2020 14:28:19: 19000000 INFO @ Wed, 22 Jul 2020 14:28:21: 10000000 INFO @ Wed, 22 Jul 2020 14:28:24: 14000000 INFO @ Wed, 22 Jul 2020 14:28:30: 20000000 INFO @ Wed, 22 Jul 2020 14:28:33: 11000000 INFO @ Wed, 22 Jul 2020 14:28:36: 15000000 INFO @ Wed, 22 Jul 2020 14:28:40: 21000000 INFO @ Wed, 22 Jul 2020 14:28:44: 12000000 INFO @ Wed, 22 Jul 2020 14:28:47: 16000000 INFO @ Wed, 22 Jul 2020 14:28:50: 22000000 INFO @ Wed, 22 Jul 2020 14:28:56: 13000000 INFO @ Wed, 22 Jul 2020 14:28:58: 17000000 INFO @ Wed, 22 Jul 2020 14:29:00: 23000000 INFO @ Wed, 22 Jul 2020 14:29:07: 14000000 INFO @ Wed, 22 Jul 2020 14:29:10: 18000000 INFO @ Wed, 22 Jul 2020 14:29:11: 24000000 INFO @ Wed, 22 Jul 2020 14:29:18: 15000000 INFO @ Wed, 22 Jul 2020 14:29:21: 25000000 INFO @ Wed, 22 Jul 2020 14:29:21: 19000000 INFO @ Wed, 22 Jul 2020 14:29:30: 16000000 INFO @ Wed, 22 Jul 2020 14:29:31: 26000000 INFO @ Wed, 22 Jul 2020 14:29:32: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:29:41: 27000000 INFO @ Wed, 22 Jul 2020 14:29:42: 17000000 INFO @ Wed, 22 Jul 2020 14:29:44: 21000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:29:51: 28000000 INFO @ Wed, 22 Jul 2020 14:29:53: 18000000 INFO @ Wed, 22 Jul 2020 14:29:55: 22000000 INFO @ Wed, 22 Jul 2020 14:30:01: 29000000 INFO @ Wed, 22 Jul 2020 14:30:05: 19000000 INFO @ Wed, 22 Jul 2020 14:30:06: 23000000 INFO @ Wed, 22 Jul 2020 14:30:12: 30000000 INFO @ Wed, 22 Jul 2020 14:30:16: 20000000 INFO @ Wed, 22 Jul 2020 14:30:18: 24000000 INFO @ Wed, 22 Jul 2020 14:30:22: 31000000 INFO @ Wed, 22 Jul 2020 14:30:28: 21000000 INFO @ Wed, 22 Jul 2020 14:30:29: 25000000 INFO @ Wed, 22 Jul 2020 14:30:32: 32000000 INFO @ Wed, 22 Jul 2020 14:30:40: 22000000 INFO @ Wed, 22 Jul 2020 14:30:41: 26000000 INFO @ Wed, 22 Jul 2020 14:30:42: 33000000 INFO @ Wed, 22 Jul 2020 14:30:52: 23000000 INFO @ Wed, 22 Jul 2020 14:30:52: 34000000 INFO @ Wed, 22 Jul 2020 14:30:52: 27000000 INFO @ Wed, 22 Jul 2020 14:31:02: 35000000 INFO @ Wed, 22 Jul 2020 14:31:04: 24000000 INFO @ Wed, 22 Jul 2020 14:31:04: 28000000 INFO @ Wed, 22 Jul 2020 14:31:13: 36000000 INFO @ Wed, 22 Jul 2020 14:31:15: 29000000 INFO @ Wed, 22 Jul 2020 14:31:16: 25000000 INFO @ Wed, 22 Jul 2020 14:31:23: 37000000 INFO @ Wed, 22 Jul 2020 14:31:27: 30000000 INFO @ Wed, 22 Jul 2020 14:31:28: 26000000 INFO @ Wed, 22 Jul 2020 14:31:34: 38000000 INFO @ Wed, 22 Jul 2020 14:31:38: 31000000 INFO @ Wed, 22 Jul 2020 14:31:40: 27000000 INFO @ Wed, 22 Jul 2020 14:31:44: 39000000 INFO @ Wed, 22 Jul 2020 14:31:50: 32000000 INFO @ Wed, 22 Jul 2020 14:31:52: 28000000 INFO @ Wed, 22 Jul 2020 14:31:54: 40000000 INFO @ Wed, 22 Jul 2020 14:32:01: 33000000 INFO @ Wed, 22 Jul 2020 14:32:04: 29000000 INFO @ Wed, 22 Jul 2020 14:32:05: 41000000 INFO @ Wed, 22 Jul 2020 14:32:13: 34000000 INFO @ Wed, 22 Jul 2020 14:32:15: 42000000 INFO @ Wed, 22 Jul 2020 14:32:16: 30000000 INFO @ Wed, 22 Jul 2020 14:32:24: 35000000 INFO @ Wed, 22 Jul 2020 14:32:25: 43000000 INFO @ Wed, 22 Jul 2020 14:32:27: 31000000 INFO @ Wed, 22 Jul 2020 14:32:35: 36000000 INFO @ Wed, 22 Jul 2020 14:32:36: 44000000 INFO @ Wed, 22 Jul 2020 14:32:39: 32000000 INFO @ Wed, 22 Jul 2020 14:32:46: 45000000 INFO @ Wed, 22 Jul 2020 14:32:47: 37000000 INFO @ Wed, 22 Jul 2020 14:32:50: 33000000 INFO @ Wed, 22 Jul 2020 14:32:56: 46000000 INFO @ Wed, 22 Jul 2020 14:32:58: 38000000 INFO @ Wed, 22 Jul 2020 14:33:02: 34000000 INFO @ Wed, 22 Jul 2020 14:33:07: 47000000 INFO @ Wed, 22 Jul 2020 14:33:10: 39000000 INFO @ Wed, 22 Jul 2020 14:33:13: 35000000 INFO @ Wed, 22 Jul 2020 14:33:17: 48000000 INFO @ Wed, 22 Jul 2020 14:33:18: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:33:18: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:33:18: #1 total tags in treatment: 17716031 INFO @ Wed, 22 Jul 2020 14:33:18: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:33:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:33:18: #1 tags after filtering in treatment: 11453831 INFO @ Wed, 22 Jul 2020 14:33:18: #1 Redundant rate of treatment: 0.35 INFO @ Wed, 22 Jul 2020 14:33:18: #1 finished! INFO @ Wed, 22 Jul 2020 14:33:18: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:33:18: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:33:19: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:33:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:33:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:33:21: 40000000 INFO @ Wed, 22 Jul 2020 14:33:25: 36000000 INFO @ Wed, 22 Jul 2020 14:33:32: 41000000 INFO @ Wed, 22 Jul 2020 14:33:36: 37000000 INFO @ Wed, 22 Jul 2020 14:33:43: 42000000 INFO @ Wed, 22 Jul 2020 14:33:48: 38000000 INFO @ Wed, 22 Jul 2020 14:33:55: 43000000 INFO @ Wed, 22 Jul 2020 14:33:59: 39000000 INFO @ Wed, 22 Jul 2020 14:34:06: 44000000 INFO @ Wed, 22 Jul 2020 14:34:10: 40000000 INFO @ Wed, 22 Jul 2020 14:34:17: 45000000 INFO @ Wed, 22 Jul 2020 14:34:22: 41000000 INFO @ Wed, 22 Jul 2020 14:34:29: 46000000 INFO @ Wed, 22 Jul 2020 14:34:33: 42000000 INFO @ Wed, 22 Jul 2020 14:34:40: 47000000 INFO @ Wed, 22 Jul 2020 14:34:45: 43000000 INFO @ Wed, 22 Jul 2020 14:34:51: 48000000 INFO @ Wed, 22 Jul 2020 14:34:52: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:34:52: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:34:52: #1 total tags in treatment: 17716031 INFO @ Wed, 22 Jul 2020 14:34:52: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:34:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:34:52: #1 tags after filtering in treatment: 11453831 INFO @ Wed, 22 Jul 2020 14:34:52: #1 Redundant rate of treatment: 0.35 INFO @ Wed, 22 Jul 2020 14:34:52: #1 finished! INFO @ Wed, 22 Jul 2020 14:34:52: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:34:52: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:34:53: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:34:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:34:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:34:56: 44000000 INFO @ Wed, 22 Jul 2020 14:35:07: 45000000 INFO @ Wed, 22 Jul 2020 14:35:17: 46000000 INFO @ Wed, 22 Jul 2020 14:35:28: 47000000 INFO @ Wed, 22 Jul 2020 14:35:38: 48000000 INFO @ Wed, 22 Jul 2020 14:35:39: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:35:39: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:35:39: #1 total tags in treatment: 17716031 INFO @ Wed, 22 Jul 2020 14:35:39: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:35:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:35:40: #1 tags after filtering in treatment: 11453831 INFO @ Wed, 22 Jul 2020 14:35:40: #1 Redundant rate of treatment: 0.35 INFO @ Wed, 22 Jul 2020 14:35:40: #1 finished! INFO @ Wed, 22 Jul 2020 14:35:40: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:35:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:35:40: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:35:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:35:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217647/SRX7217647.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling