Job ID = 7107974 SRX = SRX7217645 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28009121 spots for SRR10533722/SRR10533722.sra Written 28009121 spots for SRR10533722/SRR10533722.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:26 28009121 reads; of these: 28009121 (100.00%) were paired; of these: 9505710 (33.94%) aligned concordantly 0 times 15793456 (56.39%) aligned concordantly exactly 1 time 2709955 (9.68%) aligned concordantly >1 times ---- 9505710 pairs aligned concordantly 0 times; of these: 2221054 (23.37%) aligned discordantly 1 time ---- 7284656 pairs aligned 0 times concordantly or discordantly; of these: 14569312 mates make up the pairs; of these: 13098200 (89.90%) aligned 0 times 514916 (3.53%) aligned exactly 1 time 956196 (6.56%) aligned >1 times 76.62% overall alignment rate Time searching: 00:35:26 Overall time: 00:35:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4342879 / 20628801 = 0.2105 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:09:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:09:35: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:09:35: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:09:45: 1000000 INFO @ Wed, 22 Jul 2020 14:09:56: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:10:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:10:05: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:10:05: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:10:06: 3000000 INFO @ Wed, 22 Jul 2020 14:10:16: 1000000 INFO @ Wed, 22 Jul 2020 14:10:17: 4000000 INFO @ Wed, 22 Jul 2020 14:10:26: 2000000 INFO @ Wed, 22 Jul 2020 14:10:28: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:10:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:10:35: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:10:35: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:10:36: 3000000 INFO @ Wed, 22 Jul 2020 14:10:39: 6000000 INFO @ Wed, 22 Jul 2020 14:10:46: 1000000 INFO @ Wed, 22 Jul 2020 14:10:46: 4000000 INFO @ Wed, 22 Jul 2020 14:10:51: 7000000 INFO @ Wed, 22 Jul 2020 14:10:56: 2000000 INFO @ Wed, 22 Jul 2020 14:10:57: 5000000 INFO @ Wed, 22 Jul 2020 14:11:02: 8000000 INFO @ Wed, 22 Jul 2020 14:11:06: 3000000 INFO @ Wed, 22 Jul 2020 14:11:07: 6000000 INFO @ Wed, 22 Jul 2020 14:11:14: 9000000 INFO @ Wed, 22 Jul 2020 14:11:17: 4000000 INFO @ Wed, 22 Jul 2020 14:11:18: 7000000 INFO @ Wed, 22 Jul 2020 14:11:25: 10000000 INFO @ Wed, 22 Jul 2020 14:11:27: 5000000 INFO @ Wed, 22 Jul 2020 14:11:28: 8000000 INFO @ Wed, 22 Jul 2020 14:11:37: 11000000 INFO @ Wed, 22 Jul 2020 14:11:37: 6000000 INFO @ Wed, 22 Jul 2020 14:11:38: 9000000 INFO @ Wed, 22 Jul 2020 14:11:47: 7000000 INFO @ Wed, 22 Jul 2020 14:11:48: 12000000 INFO @ Wed, 22 Jul 2020 14:11:49: 10000000 INFO @ Wed, 22 Jul 2020 14:11:58: 8000000 INFO @ Wed, 22 Jul 2020 14:11:59: 11000000 INFO @ Wed, 22 Jul 2020 14:12:00: 13000000 INFO @ Wed, 22 Jul 2020 14:12:08: 9000000 INFO @ Wed, 22 Jul 2020 14:12:10: 12000000 INFO @ Wed, 22 Jul 2020 14:12:11: 14000000 INFO @ Wed, 22 Jul 2020 14:12:19: 10000000 INFO @ Wed, 22 Jul 2020 14:12:20: 13000000 INFO @ Wed, 22 Jul 2020 14:12:23: 15000000 INFO @ Wed, 22 Jul 2020 14:12:29: 11000000 INFO @ Wed, 22 Jul 2020 14:12:31: 14000000 INFO @ Wed, 22 Jul 2020 14:12:34: 16000000 INFO @ Wed, 22 Jul 2020 14:12:39: 12000000 INFO @ Wed, 22 Jul 2020 14:12:41: 15000000 INFO @ Wed, 22 Jul 2020 14:12:46: 17000000 INFO @ Wed, 22 Jul 2020 14:12:49: 13000000 INFO @ Wed, 22 Jul 2020 14:12:52: 16000000 INFO @ Wed, 22 Jul 2020 14:12:57: 18000000 INFO @ Wed, 22 Jul 2020 14:13:00: 14000000 INFO @ Wed, 22 Jul 2020 14:13:02: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:13:09: 19000000 INFO @ Wed, 22 Jul 2020 14:13:10: 15000000 INFO @ Wed, 22 Jul 2020 14:13:12: 18000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:13:20: 20000000 INFO @ Wed, 22 Jul 2020 14:13:21: 16000000 INFO @ Wed, 22 Jul 2020 14:13:23: 19000000 INFO @ Wed, 22 Jul 2020 14:13:31: 17000000 INFO @ Wed, 22 Jul 2020 14:13:32: 21000000 INFO @ Wed, 22 Jul 2020 14:13:33: 20000000 INFO @ Wed, 22 Jul 2020 14:13:42: 18000000 INFO @ Wed, 22 Jul 2020 14:13:43: 22000000 INFO @ Wed, 22 Jul 2020 14:13:44: 21000000 INFO @ Wed, 22 Jul 2020 14:13:52: 19000000 INFO @ Wed, 22 Jul 2020 14:13:55: 23000000 INFO @ Wed, 22 Jul 2020 14:13:55: 22000000 INFO @ Wed, 22 Jul 2020 14:14:03: 20000000 INFO @ Wed, 22 Jul 2020 14:14:06: 23000000 INFO @ Wed, 22 Jul 2020 14:14:06: 24000000 INFO @ Wed, 22 Jul 2020 14:14:14: 21000000 INFO @ Wed, 22 Jul 2020 14:14:16: 24000000 INFO @ Wed, 22 Jul 2020 14:14:18: 25000000 INFO @ Wed, 22 Jul 2020 14:14:25: 22000000 INFO @ Wed, 22 Jul 2020 14:14:27: 25000000 INFO @ Wed, 22 Jul 2020 14:14:30: 26000000 INFO @ Wed, 22 Jul 2020 14:14:36: 23000000 INFO @ Wed, 22 Jul 2020 14:14:38: 26000000 INFO @ Wed, 22 Jul 2020 14:14:41: 27000000 INFO @ Wed, 22 Jul 2020 14:14:46: 24000000 INFO @ Wed, 22 Jul 2020 14:14:49: 27000000 INFO @ Wed, 22 Jul 2020 14:14:53: 28000000 INFO @ Wed, 22 Jul 2020 14:14:57: 25000000 INFO @ Wed, 22 Jul 2020 14:14:59: 28000000 INFO @ Wed, 22 Jul 2020 14:15:04: 29000000 INFO @ Wed, 22 Jul 2020 14:15:08: 26000000 INFO @ Wed, 22 Jul 2020 14:15:10: 29000000 INFO @ Wed, 22 Jul 2020 14:15:16: 30000000 INFO @ Wed, 22 Jul 2020 14:15:19: 27000000 INFO @ Wed, 22 Jul 2020 14:15:21: 30000000 INFO @ Wed, 22 Jul 2020 14:15:27: 31000000 INFO @ Wed, 22 Jul 2020 14:15:29: 28000000 INFO @ Wed, 22 Jul 2020 14:15:31: 31000000 INFO @ Wed, 22 Jul 2020 14:15:39: 32000000 INFO @ Wed, 22 Jul 2020 14:15:40: 29000000 INFO @ Wed, 22 Jul 2020 14:15:42: 32000000 INFO @ Wed, 22 Jul 2020 14:15:51: 33000000 INFO @ Wed, 22 Jul 2020 14:15:51: 30000000 INFO @ Wed, 22 Jul 2020 14:15:53: 33000000 INFO @ Wed, 22 Jul 2020 14:16:02: 34000000 INFO @ Wed, 22 Jul 2020 14:16:02: 31000000 INFO @ Wed, 22 Jul 2020 14:16:04: 34000000 INFO @ Wed, 22 Jul 2020 14:16:05: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:16:05: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:16:05: #1 total tags in treatment: 14621905 INFO @ Wed, 22 Jul 2020 14:16:05: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:16:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:16:05: #1 tags after filtering in treatment: 9722338 INFO @ Wed, 22 Jul 2020 14:16:05: #1 Redundant rate of treatment: 0.34 INFO @ Wed, 22 Jul 2020 14:16:05: #1 finished! INFO @ Wed, 22 Jul 2020 14:16:05: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:16:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:16:06: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:16:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:16:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:16:06: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:16:06: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:16:06: #1 total tags in treatment: 14621905 INFO @ Wed, 22 Jul 2020 14:16:06: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:16:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:16:06: #1 tags after filtering in treatment: 9722338 INFO @ Wed, 22 Jul 2020 14:16:06: #1 Redundant rate of treatment: 0.34 INFO @ Wed, 22 Jul 2020 14:16:06: #1 finished! INFO @ Wed, 22 Jul 2020 14:16:06: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:16:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:16:07: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:16:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:16:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:16:12: 32000000 INFO @ Wed, 22 Jul 2020 14:16:22: 33000000 INFO @ Wed, 22 Jul 2020 14:16:31: 34000000 INFO @ Wed, 22 Jul 2020 14:16:34: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:16:34: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:16:34: #1 total tags in treatment: 14621905 INFO @ Wed, 22 Jul 2020 14:16:34: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:16:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:16:34: #1 tags after filtering in treatment: 9722338 INFO @ Wed, 22 Jul 2020 14:16:34: #1 Redundant rate of treatment: 0.34 INFO @ Wed, 22 Jul 2020 14:16:34: #1 finished! INFO @ Wed, 22 Jul 2020 14:16:34: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:16:34: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:16:35: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:16:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:16:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217645/SRX7217645.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling