Job ID = 7107924 SRX = SRX7217643 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 29634426 spots for SRR10533720/SRR10533720.sra Written 29634426 spots for SRR10533720/SRR10533720.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:38:03 29634426 reads; of these: 29634426 (100.00%) were paired; of these: 8235628 (27.79%) aligned concordantly 0 times 17989002 (60.70%) aligned concordantly exactly 1 time 3409796 (11.51%) aligned concordantly >1 times ---- 8235628 pairs aligned concordantly 0 times; of these: 4761088 (57.81%) aligned discordantly 1 time ---- 3474540 pairs aligned 0 times concordantly or discordantly; of these: 6949080 mates make up the pairs; of these: 3657808 (52.64%) aligned 0 times 1061857 (15.28%) aligned exactly 1 time 2229415 (32.08%) aligned >1 times 93.83% overall alignment rate Time searching: 00:38:03 Overall time: 00:38:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4345427 / 25297286 = 0.1718 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:13:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:13:47: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:13:47: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:13:54: 1000000 INFO @ Wed, 22 Jul 2020 14:14:01: 2000000 INFO @ Wed, 22 Jul 2020 14:14:08: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:14:15: 4000000 INFO @ Wed, 22 Jul 2020 14:14:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:14:17: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:14:17: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:14:23: 5000000 INFO @ Wed, 22 Jul 2020 14:14:25: 1000000 INFO @ Wed, 22 Jul 2020 14:14:30: 6000000 INFO @ Wed, 22 Jul 2020 14:14:32: 2000000 INFO @ Wed, 22 Jul 2020 14:14:37: 7000000 INFO @ Wed, 22 Jul 2020 14:14:40: 3000000 INFO @ Wed, 22 Jul 2020 14:14:44: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:14:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:14:47: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:14:47: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:14:48: 4000000 INFO @ Wed, 22 Jul 2020 14:14:52: 9000000 INFO @ Wed, 22 Jul 2020 14:14:55: 1000000 INFO @ Wed, 22 Jul 2020 14:14:55: 5000000 INFO @ Wed, 22 Jul 2020 14:14:59: 10000000 INFO @ Wed, 22 Jul 2020 14:15:02: 2000000 INFO @ Wed, 22 Jul 2020 14:15:03: 6000000 INFO @ Wed, 22 Jul 2020 14:15:06: 11000000 INFO @ Wed, 22 Jul 2020 14:15:10: 3000000 INFO @ Wed, 22 Jul 2020 14:15:11: 7000000 INFO @ Wed, 22 Jul 2020 14:15:14: 12000000 INFO @ Wed, 22 Jul 2020 14:15:18: 4000000 INFO @ Wed, 22 Jul 2020 14:15:18: 8000000 INFO @ Wed, 22 Jul 2020 14:15:21: 13000000 INFO @ Wed, 22 Jul 2020 14:15:26: 5000000 INFO @ Wed, 22 Jul 2020 14:15:26: 9000000 INFO @ Wed, 22 Jul 2020 14:15:28: 14000000 INFO @ Wed, 22 Jul 2020 14:15:34: 6000000 INFO @ Wed, 22 Jul 2020 14:15:34: 10000000 INFO @ Wed, 22 Jul 2020 14:15:36: 15000000 INFO @ Wed, 22 Jul 2020 14:15:41: 7000000 INFO @ Wed, 22 Jul 2020 14:15:42: 11000000 INFO @ Wed, 22 Jul 2020 14:15:43: 16000000 INFO @ Wed, 22 Jul 2020 14:15:49: 12000000 INFO @ Wed, 22 Jul 2020 14:15:49: 8000000 INFO @ Wed, 22 Jul 2020 14:15:50: 17000000 INFO @ Wed, 22 Jul 2020 14:15:57: 13000000 INFO @ Wed, 22 Jul 2020 14:15:57: 9000000 INFO @ Wed, 22 Jul 2020 14:15:57: 18000000 INFO @ Wed, 22 Jul 2020 14:16:04: 14000000 INFO @ Wed, 22 Jul 2020 14:16:05: 19000000 INFO @ Wed, 22 Jul 2020 14:16:05: 10000000 INFO @ Wed, 22 Jul 2020 14:16:12: 15000000 INFO @ Wed, 22 Jul 2020 14:16:12: 20000000 INFO @ Wed, 22 Jul 2020 14:16:13: 11000000 INFO @ Wed, 22 Jul 2020 14:16:19: 16000000 INFO @ Wed, 22 Jul 2020 14:16:20: 21000000 INFO @ Wed, 22 Jul 2020 14:16:20: 12000000 INFO @ Wed, 22 Jul 2020 14:16:26: 17000000 INFO @ Wed, 22 Jul 2020 14:16:27: 22000000 INFO @ Wed, 22 Jul 2020 14:16:28: 13000000 INFO @ Wed, 22 Jul 2020 14:16:34: 18000000 INFO @ Wed, 22 Jul 2020 14:16:35: 23000000 INFO @ Wed, 22 Jul 2020 14:16:35: 14000000 INFO @ Wed, 22 Jul 2020 14:16:41: 19000000 INFO @ Wed, 22 Jul 2020 14:16:42: 24000000 INFO @ Wed, 22 Jul 2020 14:16:43: 15000000 INFO @ Wed, 22 Jul 2020 14:16:49: 20000000 INFO @ Wed, 22 Jul 2020 14:16:49: 25000000 INFO @ Wed, 22 Jul 2020 14:16:50: 16000000 INFO @ Wed, 22 Jul 2020 14:16:57: 26000000 INFO @ Wed, 22 Jul 2020 14:16:57: 21000000 INFO @ Wed, 22 Jul 2020 14:16:57: 17000000 INFO @ Wed, 22 Jul 2020 14:17:04: 27000000 INFO @ Wed, 22 Jul 2020 14:17:05: 22000000 INFO @ Wed, 22 Jul 2020 14:17:05: 18000000 INFO @ Wed, 22 Jul 2020 14:17:11: 28000000 INFO @ Wed, 22 Jul 2020 14:17:12: 23000000 INFO @ Wed, 22 Jul 2020 14:17:12: 19000000 INFO @ Wed, 22 Jul 2020 14:17:18: 29000000 INFO @ Wed, 22 Jul 2020 14:17:18: 24000000 INFO @ Wed, 22 Jul 2020 14:17:20: 20000000 INFO @ Wed, 22 Jul 2020 14:17:26: 30000000 INFO @ Wed, 22 Jul 2020 14:17:26: 25000000 INFO @ Wed, 22 Jul 2020 14:17:28: 21000000 INFO @ Wed, 22 Jul 2020 14:17:33: 31000000 INFO @ Wed, 22 Jul 2020 14:17:33: 26000000 INFO @ Wed, 22 Jul 2020 14:17:35: 22000000 INFO @ Wed, 22 Jul 2020 14:17:40: 32000000 INFO @ Wed, 22 Jul 2020 14:17:41: 27000000 INFO @ Wed, 22 Jul 2020 14:17:43: 23000000 INFO @ Wed, 22 Jul 2020 14:17:48: 33000000 INFO @ Wed, 22 Jul 2020 14:17:48: 28000000 INFO @ Wed, 22 Jul 2020 14:17:50: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:17:56: 29000000 INFO @ Wed, 22 Jul 2020 14:17:56: 34000000 INFO @ Wed, 22 Jul 2020 14:17:57: 25000000 INFO @ Wed, 22 Jul 2020 14:18:03: 30000000 INFO @ Wed, 22 Jul 2020 14:18:03: 35000000 INFO @ Wed, 22 Jul 2020 14:18:05: 26000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:18:11: 31000000 INFO @ Wed, 22 Jul 2020 14:18:11: 36000000 INFO @ Wed, 22 Jul 2020 14:18:12: 27000000 INFO @ Wed, 22 Jul 2020 14:18:18: 32000000 INFO @ Wed, 22 Jul 2020 14:18:19: 37000000 INFO @ Wed, 22 Jul 2020 14:18:20: 28000000 INFO @ Wed, 22 Jul 2020 14:18:25: 33000000 INFO @ Wed, 22 Jul 2020 14:18:27: 38000000 INFO @ Wed, 22 Jul 2020 14:18:27: 29000000 INFO @ Wed, 22 Jul 2020 14:18:33: 34000000 INFO @ Wed, 22 Jul 2020 14:18:34: 39000000 INFO @ Wed, 22 Jul 2020 14:18:35: 30000000 INFO @ Wed, 22 Jul 2020 14:18:41: 35000000 INFO @ Wed, 22 Jul 2020 14:18:42: 31000000 INFO @ Wed, 22 Jul 2020 14:18:42: 40000000 INFO @ Wed, 22 Jul 2020 14:18:49: 36000000 INFO @ Wed, 22 Jul 2020 14:18:49: 32000000 INFO @ Wed, 22 Jul 2020 14:18:50: 41000000 INFO @ Wed, 22 Jul 2020 14:18:57: 33000000 INFO @ Wed, 22 Jul 2020 14:18:57: 37000000 INFO @ Wed, 22 Jul 2020 14:18:57: 42000000 INFO @ Wed, 22 Jul 2020 14:19:05: 34000000 INFO @ Wed, 22 Jul 2020 14:19:05: 38000000 INFO @ Wed, 22 Jul 2020 14:19:05: 43000000 INFO @ Wed, 22 Jul 2020 14:19:12: 35000000 INFO @ Wed, 22 Jul 2020 14:19:12: 39000000 INFO @ Wed, 22 Jul 2020 14:19:13: 44000000 INFO @ Wed, 22 Jul 2020 14:19:20: 36000000 INFO @ Wed, 22 Jul 2020 14:19:20: 40000000 INFO @ Wed, 22 Jul 2020 14:19:21: 45000000 INFO @ Wed, 22 Jul 2020 14:19:27: 41000000 INFO @ Wed, 22 Jul 2020 14:19:27: 37000000 INFO @ Wed, 22 Jul 2020 14:19:29: 46000000 INFO @ Wed, 22 Jul 2020 14:19:35: 38000000 INFO @ Wed, 22 Jul 2020 14:19:35: 42000000 INFO @ Wed, 22 Jul 2020 14:19:36: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:19:36: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:19:36: #1 total tags in treatment: 17701301 INFO @ Wed, 22 Jul 2020 14:19:36: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:19:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:19:37: #1 tags after filtering in treatment: 11328409 INFO @ Wed, 22 Jul 2020 14:19:37: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 14:19:37: #1 finished! INFO @ Wed, 22 Jul 2020 14:19:37: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:19:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:19:37: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:19:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:19:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:19:42: 43000000 INFO @ Wed, 22 Jul 2020 14:19:42: 39000000 INFO @ Wed, 22 Jul 2020 14:19:50: 40000000 INFO @ Wed, 22 Jul 2020 14:19:50: 44000000 INFO @ Wed, 22 Jul 2020 14:19:57: 41000000 INFO @ Wed, 22 Jul 2020 14:19:58: 45000000 INFO @ Wed, 22 Jul 2020 14:20:05: 42000000 INFO @ Wed, 22 Jul 2020 14:20:06: 46000000 INFO @ Wed, 22 Jul 2020 14:20:12: 43000000 INFO @ Wed, 22 Jul 2020 14:20:13: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:20:13: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:20:13: #1 total tags in treatment: 17701301 INFO @ Wed, 22 Jul 2020 14:20:13: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:20:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:20:13: #1 tags after filtering in treatment: 11328409 INFO @ Wed, 22 Jul 2020 14:20:13: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 14:20:13: #1 finished! INFO @ Wed, 22 Jul 2020 14:20:13: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:20:13: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:20:14: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:20:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:20:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:20:20: 44000000 INFO @ Wed, 22 Jul 2020 14:20:27: 45000000 INFO @ Wed, 22 Jul 2020 14:20:35: 46000000 INFO @ Wed, 22 Jul 2020 14:20:42: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:20:42: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:20:42: #1 total tags in treatment: 17701301 INFO @ Wed, 22 Jul 2020 14:20:42: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:20:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:20:42: #1 tags after filtering in treatment: 11328409 INFO @ Wed, 22 Jul 2020 14:20:42: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 14:20:42: #1 finished! INFO @ Wed, 22 Jul 2020 14:20:42: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:20:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:20:43: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:20:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:20:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217643/SRX7217643.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling