Job ID = 7107906 SRX = SRX7217641 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18689017 spots for SRR10533718/SRR10533718.sra Written 18689017 spots for SRR10533718/SRR10533718.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:13 18689017 reads; of these: 18689017 (100.00%) were paired; of these: 7889166 (42.21%) aligned concordantly 0 times 9067551 (48.52%) aligned concordantly exactly 1 time 1732300 (9.27%) aligned concordantly >1 times ---- 7889166 pairs aligned concordantly 0 times; of these: 3555191 (45.06%) aligned discordantly 1 time ---- 4333975 pairs aligned 0 times concordantly or discordantly; of these: 8667950 mates make up the pairs; of these: 6957977 (80.27%) aligned 0 times 373960 (4.31%) aligned exactly 1 time 1336013 (15.41%) aligned >1 times 81.38% overall alignment rate Time searching: 00:23:13 Overall time: 00:23:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2319271 / 14224322 = 0.1630 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:50:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:50:25: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:50:25: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:50:34: 1000000 INFO @ Wed, 22 Jul 2020 13:50:43: 2000000 INFO @ Wed, 22 Jul 2020 13:50:52: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:50:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:50:55: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:50:55: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:51:01: 4000000 INFO @ Wed, 22 Jul 2020 13:51:07: 1000000 INFO @ Wed, 22 Jul 2020 13:51:11: 5000000 INFO @ Wed, 22 Jul 2020 13:51:18: 2000000 INFO @ Wed, 22 Jul 2020 13:51:23: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:51:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:51:26: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:51:26: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:51:29: 3000000 INFO @ Wed, 22 Jul 2020 13:51:34: 7000000 INFO @ Wed, 22 Jul 2020 13:51:37: 1000000 INFO @ Wed, 22 Jul 2020 13:51:41: 4000000 INFO @ Wed, 22 Jul 2020 13:51:45: 8000000 INFO @ Wed, 22 Jul 2020 13:51:50: 2000000 INFO @ Wed, 22 Jul 2020 13:51:53: 5000000 INFO @ Wed, 22 Jul 2020 13:51:55: 9000000 INFO @ Wed, 22 Jul 2020 13:52:01: 3000000 INFO @ Wed, 22 Jul 2020 13:52:03: 6000000 INFO @ Wed, 22 Jul 2020 13:52:05: 10000000 INFO @ Wed, 22 Jul 2020 13:52:13: 4000000 INFO @ Wed, 22 Jul 2020 13:52:14: 7000000 INFO @ Wed, 22 Jul 2020 13:52:16: 11000000 INFO @ Wed, 22 Jul 2020 13:52:24: 5000000 INFO @ Wed, 22 Jul 2020 13:52:25: 8000000 INFO @ Wed, 22 Jul 2020 13:52:26: 12000000 INFO @ Wed, 22 Jul 2020 13:52:35: 6000000 INFO @ Wed, 22 Jul 2020 13:52:36: 13000000 INFO @ Wed, 22 Jul 2020 13:52:36: 9000000 INFO @ Wed, 22 Jul 2020 13:52:45: 7000000 INFO @ Wed, 22 Jul 2020 13:52:47: 14000000 INFO @ Wed, 22 Jul 2020 13:52:48: 10000000 INFO @ Wed, 22 Jul 2020 13:52:56: 8000000 INFO @ Wed, 22 Jul 2020 13:52:57: 15000000 INFO @ Wed, 22 Jul 2020 13:52:59: 11000000 INFO @ Wed, 22 Jul 2020 13:53:07: 16000000 INFO @ Wed, 22 Jul 2020 13:53:08: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:53:11: 12000000 INFO @ Wed, 22 Jul 2020 13:53:17: 17000000 INFO @ Wed, 22 Jul 2020 13:53:21: 10000000 INFO @ Wed, 22 Jul 2020 13:53:24: 13000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:53:27: 18000000 INFO @ Wed, 22 Jul 2020 13:53:33: 11000000 INFO @ Wed, 22 Jul 2020 13:53:35: 14000000 INFO @ Wed, 22 Jul 2020 13:53:37: 19000000 INFO @ Wed, 22 Jul 2020 13:53:45: 12000000 INFO @ Wed, 22 Jul 2020 13:53:47: 20000000 INFO @ Wed, 22 Jul 2020 13:53:47: 15000000 INFO @ Wed, 22 Jul 2020 13:53:57: 13000000 INFO @ Wed, 22 Jul 2020 13:53:57: 21000000 INFO @ Wed, 22 Jul 2020 13:53:59: 16000000 INFO @ Wed, 22 Jul 2020 13:54:08: 14000000 INFO @ Wed, 22 Jul 2020 13:54:09: 22000000 INFO @ Wed, 22 Jul 2020 13:54:11: 17000000 INFO @ Wed, 22 Jul 2020 13:54:20: 23000000 INFO @ Wed, 22 Jul 2020 13:54:20: 15000000 INFO @ Wed, 22 Jul 2020 13:54:23: 18000000 INFO @ Wed, 22 Jul 2020 13:54:31: 24000000 INFO @ Wed, 22 Jul 2020 13:54:32: 16000000 INFO @ Wed, 22 Jul 2020 13:54:35: 19000000 INFO @ Wed, 22 Jul 2020 13:54:41: 25000000 INFO @ Wed, 22 Jul 2020 13:54:44: 17000000 INFO @ Wed, 22 Jul 2020 13:54:47: 20000000 INFO @ Wed, 22 Jul 2020 13:54:50: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:54:50: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:54:50: #1 total tags in treatment: 8964220 INFO @ Wed, 22 Jul 2020 13:54:50: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:54:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:54:50: #1 tags after filtering in treatment: 6487586 INFO @ Wed, 22 Jul 2020 13:54:50: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 22 Jul 2020 13:54:50: #1 finished! INFO @ Wed, 22 Jul 2020 13:54:50: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:54:50: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:54:50: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:54:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:54:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:54:56: 18000000 INFO @ Wed, 22 Jul 2020 13:54:58: 21000000 INFO @ Wed, 22 Jul 2020 13:55:06: 19000000 INFO @ Wed, 22 Jul 2020 13:55:07: 22000000 INFO @ Wed, 22 Jul 2020 13:55:17: 20000000 INFO @ Wed, 22 Jul 2020 13:55:17: 23000000 INFO @ Wed, 22 Jul 2020 13:55:27: 21000000 INFO @ Wed, 22 Jul 2020 13:55:27: 24000000 INFO @ Wed, 22 Jul 2020 13:55:36: 22000000 INFO @ Wed, 22 Jul 2020 13:55:37: 25000000 INFO @ Wed, 22 Jul 2020 13:55:44: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:55:44: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:55:44: #1 total tags in treatment: 8964220 INFO @ Wed, 22 Jul 2020 13:55:44: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:55:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:55:44: #1 tags after filtering in treatment: 6487586 INFO @ Wed, 22 Jul 2020 13:55:44: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 22 Jul 2020 13:55:44: #1 finished! INFO @ Wed, 22 Jul 2020 13:55:44: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:55:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:55:44: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:55:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:55:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:55:45: 23000000 INFO @ Wed, 22 Jul 2020 13:55:54: 24000000 INFO @ Wed, 22 Jul 2020 13:56:03: 25000000 INFO @ Wed, 22 Jul 2020 13:56:10: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:56:10: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:56:10: #1 total tags in treatment: 8964220 INFO @ Wed, 22 Jul 2020 13:56:10: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:56:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:56:10: #1 tags after filtering in treatment: 6487586 INFO @ Wed, 22 Jul 2020 13:56:10: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 22 Jul 2020 13:56:10: #1 finished! INFO @ Wed, 22 Jul 2020 13:56:10: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:56:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:56:10: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:56:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:56:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217641/SRX7217641.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling