Job ID = 7107829 SRX = SRX7217639 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17198197 spots for SRR10533716/SRR10533716.sra Written 17198197 spots for SRR10533716/SRR10533716.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:10 17198197 reads; of these: 17198197 (100.00%) were paired; of these: 3673897 (21.36%) aligned concordantly 0 times 11132869 (64.73%) aligned concordantly exactly 1 time 2391431 (13.91%) aligned concordantly >1 times ---- 3673897 pairs aligned concordantly 0 times; of these: 2172748 (59.14%) aligned discordantly 1 time ---- 1501149 pairs aligned 0 times concordantly or discordantly; of these: 3002298 mates make up the pairs; of these: 1474935 (49.13%) aligned 0 times 422720 (14.08%) aligned exactly 1 time 1104643 (36.79%) aligned >1 times 95.71% overall alignment rate Time searching: 00:22:10 Overall time: 00:22:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2774093 / 15345811 = 0.1808 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:48:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:48:29: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:48:29: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:48:37: 1000000 INFO @ Wed, 22 Jul 2020 13:48:44: 2000000 INFO @ Wed, 22 Jul 2020 13:48:52: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:48:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:48:59: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:48:59: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:48:59: 4000000 INFO @ Wed, 22 Jul 2020 13:49:08: 5000000 INFO @ Wed, 22 Jul 2020 13:49:09: 1000000 INFO @ Wed, 22 Jul 2020 13:49:17: 6000000 INFO @ Wed, 22 Jul 2020 13:49:18: 2000000 INFO @ Wed, 22 Jul 2020 13:49:26: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:49:28: 3000000 INFO @ Wed, 22 Jul 2020 13:49:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:49:29: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:49:29: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:49:35: 8000000 INFO @ Wed, 22 Jul 2020 13:49:38: 1000000 INFO @ Wed, 22 Jul 2020 13:49:38: 4000000 INFO @ Wed, 22 Jul 2020 13:49:44: 9000000 INFO @ Wed, 22 Jul 2020 13:49:47: 2000000 INFO @ Wed, 22 Jul 2020 13:49:48: 5000000 INFO @ Wed, 22 Jul 2020 13:49:53: 10000000 INFO @ Wed, 22 Jul 2020 13:49:56: 3000000 INFO @ Wed, 22 Jul 2020 13:49:58: 6000000 INFO @ Wed, 22 Jul 2020 13:50:02: 11000000 INFO @ Wed, 22 Jul 2020 13:50:05: 4000000 INFO @ Wed, 22 Jul 2020 13:50:08: 7000000 INFO @ Wed, 22 Jul 2020 13:50:11: 12000000 INFO @ Wed, 22 Jul 2020 13:50:14: 5000000 INFO @ Wed, 22 Jul 2020 13:50:18: 8000000 INFO @ Wed, 22 Jul 2020 13:50:20: 13000000 INFO @ Wed, 22 Jul 2020 13:50:23: 6000000 INFO @ Wed, 22 Jul 2020 13:50:27: 9000000 INFO @ Wed, 22 Jul 2020 13:50:29: 14000000 INFO @ Wed, 22 Jul 2020 13:50:32: 7000000 INFO @ Wed, 22 Jul 2020 13:50:37: 10000000 INFO @ Wed, 22 Jul 2020 13:50:38: 15000000 INFO @ Wed, 22 Jul 2020 13:50:41: 8000000 INFO @ Wed, 22 Jul 2020 13:50:47: 16000000 INFO @ Wed, 22 Jul 2020 13:50:47: 11000000 INFO @ Wed, 22 Jul 2020 13:50:50: 9000000 INFO @ Wed, 22 Jul 2020 13:50:56: 17000000 INFO @ Wed, 22 Jul 2020 13:50:57: 12000000 INFO @ Wed, 22 Jul 2020 13:50:58: 10000000 INFO @ Wed, 22 Jul 2020 13:51:04: 18000000 INFO @ Wed, 22 Jul 2020 13:51:07: 13000000 INFO @ Wed, 22 Jul 2020 13:51:07: 11000000 INFO @ Wed, 22 Jul 2020 13:51:13: 19000000 INFO @ Wed, 22 Jul 2020 13:51:16: 12000000 INFO @ Wed, 22 Jul 2020 13:51:17: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:51:23: 20000000 INFO @ Wed, 22 Jul 2020 13:51:25: 13000000 INFO @ Wed, 22 Jul 2020 13:51:27: 15000000 INFO @ Wed, 22 Jul 2020 13:51:32: 21000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:51:34: 14000000 INFO @ Wed, 22 Jul 2020 13:51:36: 16000000 INFO @ Wed, 22 Jul 2020 13:51:41: 22000000 INFO @ Wed, 22 Jul 2020 13:51:43: 15000000 INFO @ Wed, 22 Jul 2020 13:51:47: 17000000 INFO @ Wed, 22 Jul 2020 13:51:50: 23000000 INFO @ Wed, 22 Jul 2020 13:51:52: 16000000 INFO @ Wed, 22 Jul 2020 13:51:57: 18000000 INFO @ Wed, 22 Jul 2020 13:51:59: 24000000 INFO @ Wed, 22 Jul 2020 13:52:01: 17000000 INFO @ Wed, 22 Jul 2020 13:52:07: 19000000 INFO @ Wed, 22 Jul 2020 13:52:08: 25000000 INFO @ Wed, 22 Jul 2020 13:52:10: 18000000 INFO @ Wed, 22 Jul 2020 13:52:17: 26000000 INFO @ Wed, 22 Jul 2020 13:52:17: 20000000 INFO @ Wed, 22 Jul 2020 13:52:19: 19000000 INFO @ Wed, 22 Jul 2020 13:52:26: 27000000 INFO @ Wed, 22 Jul 2020 13:52:27: 21000000 INFO @ Wed, 22 Jul 2020 13:52:28: 20000000 INFO @ Wed, 22 Jul 2020 13:52:29: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:52:29: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:52:29: #1 total tags in treatment: 11081936 INFO @ Wed, 22 Jul 2020 13:52:29: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:52:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:52:29: #1 tags after filtering in treatment: 7977918 INFO @ Wed, 22 Jul 2020 13:52:29: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 22 Jul 2020 13:52:29: #1 finished! INFO @ Wed, 22 Jul 2020 13:52:29: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:52:29: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:52:30: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:52:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:52:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:52:37: 21000000 INFO @ Wed, 22 Jul 2020 13:52:37: 22000000 INFO @ Wed, 22 Jul 2020 13:52:46: 22000000 INFO @ Wed, 22 Jul 2020 13:52:48: 23000000 INFO @ Wed, 22 Jul 2020 13:52:55: 23000000 INFO @ Wed, 22 Jul 2020 13:52:58: 24000000 INFO @ Wed, 22 Jul 2020 13:53:04: 24000000 INFO @ Wed, 22 Jul 2020 13:53:09: 25000000 INFO @ Wed, 22 Jul 2020 13:53:13: 25000000 INFO @ Wed, 22 Jul 2020 13:53:19: 26000000 INFO @ Wed, 22 Jul 2020 13:53:21: 26000000 INFO @ Wed, 22 Jul 2020 13:53:30: 27000000 INFO @ Wed, 22 Jul 2020 13:53:31: 27000000 INFO @ Wed, 22 Jul 2020 13:53:33: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:53:33: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:53:33: #1 total tags in treatment: 11081936 INFO @ Wed, 22 Jul 2020 13:53:33: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:53:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:53:33: #1 tags after filtering in treatment: 7977918 INFO @ Wed, 22 Jul 2020 13:53:33: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 22 Jul 2020 13:53:33: #1 finished! INFO @ Wed, 22 Jul 2020 13:53:33: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:53:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:53:34: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:53:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:53:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:53:34: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:53:34: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:53:34: #1 total tags in treatment: 11081936 INFO @ Wed, 22 Jul 2020 13:53:34: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:53:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:53:34: #1 tags after filtering in treatment: 7977918 INFO @ Wed, 22 Jul 2020 13:53:34: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 22 Jul 2020 13:53:34: #1 finished! INFO @ Wed, 22 Jul 2020 13:53:34: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:53:34: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:53:35: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:53:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:53:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217639/SRX7217639.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling