Job ID = 7107825 SRX = SRX7217638 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 24631837 spots for SRR10533715/SRR10533715.sra Written 24631837 spots for SRR10533715/SRR10533715.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:15 24631837 reads; of these: 24631837 (100.00%) were paired; of these: 4899339 (19.89%) aligned concordantly 0 times 16594394 (67.37%) aligned concordantly exactly 1 time 3138104 (12.74%) aligned concordantly >1 times ---- 4899339 pairs aligned concordantly 0 times; of these: 2523108 (51.50%) aligned discordantly 1 time ---- 2376231 pairs aligned 0 times concordantly or discordantly; of these: 4752462 mates make up the pairs; of these: 2967100 (62.43%) aligned 0 times 620069 (13.05%) aligned exactly 1 time 1165293 (24.52%) aligned >1 times 93.98% overall alignment rate Time searching: 00:32:15 Overall time: 00:32:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3298801 / 21707445 = 0.1520 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:02:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:02:40: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:02:40: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:02:48: 1000000 INFO @ Wed, 22 Jul 2020 14:02:55: 2000000 INFO @ Wed, 22 Jul 2020 14:03:03: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:03:10: 4000000 INFO @ Wed, 22 Jul 2020 14:03:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:03:10: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:03:10: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:03:18: 5000000 INFO @ Wed, 22 Jul 2020 14:03:18: 1000000 INFO @ Wed, 22 Jul 2020 14:03:26: 2000000 INFO @ Wed, 22 Jul 2020 14:03:26: 6000000 INFO @ Wed, 22 Jul 2020 14:03:34: 3000000 INFO @ Wed, 22 Jul 2020 14:03:34: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:03:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:03:40: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:03:40: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:03:42: 4000000 INFO @ Wed, 22 Jul 2020 14:03:42: 8000000 INFO @ Wed, 22 Jul 2020 14:03:47: 1000000 INFO @ Wed, 22 Jul 2020 14:03:50: 5000000 INFO @ Wed, 22 Jul 2020 14:03:50: 9000000 INFO @ Wed, 22 Jul 2020 14:03:54: 2000000 INFO @ Wed, 22 Jul 2020 14:03:58: 6000000 INFO @ Wed, 22 Jul 2020 14:03:59: 10000000 INFO @ Wed, 22 Jul 2020 14:04:00: 3000000 INFO @ Wed, 22 Jul 2020 14:04:07: 7000000 INFO @ Wed, 22 Jul 2020 14:04:07: 4000000 INFO @ Wed, 22 Jul 2020 14:04:07: 11000000 INFO @ Wed, 22 Jul 2020 14:04:14: 5000000 INFO @ Wed, 22 Jul 2020 14:04:15: 8000000 INFO @ Wed, 22 Jul 2020 14:04:16: 12000000 INFO @ Wed, 22 Jul 2020 14:04:20: 6000000 INFO @ Wed, 22 Jul 2020 14:04:24: 9000000 INFO @ Wed, 22 Jul 2020 14:04:24: 13000000 INFO @ Wed, 22 Jul 2020 14:04:27: 7000000 INFO @ Wed, 22 Jul 2020 14:04:32: 10000000 INFO @ Wed, 22 Jul 2020 14:04:33: 14000000 INFO @ Wed, 22 Jul 2020 14:04:33: 8000000 INFO @ Wed, 22 Jul 2020 14:04:40: 9000000 INFO @ Wed, 22 Jul 2020 14:04:41: 11000000 INFO @ Wed, 22 Jul 2020 14:04:41: 15000000 INFO @ Wed, 22 Jul 2020 14:04:47: 10000000 INFO @ Wed, 22 Jul 2020 14:04:49: 12000000 INFO @ Wed, 22 Jul 2020 14:04:50: 16000000 INFO @ Wed, 22 Jul 2020 14:04:53: 11000000 INFO @ Wed, 22 Jul 2020 14:04:58: 13000000 INFO @ Wed, 22 Jul 2020 14:04:58: 17000000 INFO @ Wed, 22 Jul 2020 14:05:00: 12000000 INFO @ Wed, 22 Jul 2020 14:05:06: 13000000 INFO @ Wed, 22 Jul 2020 14:05:07: 14000000 INFO @ Wed, 22 Jul 2020 14:05:07: 18000000 INFO @ Wed, 22 Jul 2020 14:05:13: 14000000 INFO @ Wed, 22 Jul 2020 14:05:15: 15000000 INFO @ Wed, 22 Jul 2020 14:05:15: 19000000 INFO @ Wed, 22 Jul 2020 14:05:20: 15000000 INFO @ Wed, 22 Jul 2020 14:05:24: 16000000 INFO @ Wed, 22 Jul 2020 14:05:24: 20000000 INFO @ Wed, 22 Jul 2020 14:05:26: 16000000 INFO @ Wed, 22 Jul 2020 14:05:32: 17000000 INFO @ Wed, 22 Jul 2020 14:05:32: 21000000 INFO @ Wed, 22 Jul 2020 14:05:33: 17000000 INFO @ Wed, 22 Jul 2020 14:05:41: 18000000 INFO @ Wed, 22 Jul 2020 14:05:41: 22000000 INFO @ Wed, 22 Jul 2020 14:05:42: 18000000 INFO @ Wed, 22 Jul 2020 14:05:49: 19000000 INFO @ Wed, 22 Jul 2020 14:05:49: 23000000 INFO @ Wed, 22 Jul 2020 14:05:50: 19000000 INFO @ Wed, 22 Jul 2020 14:05:57: 24000000 INFO @ Wed, 22 Jul 2020 14:05:57: 20000000 INFO @ Wed, 22 Jul 2020 14:05:58: 20000000 INFO @ Wed, 22 Jul 2020 14:06:06: 25000000 INFO @ Wed, 22 Jul 2020 14:06:06: 21000000 INFO @ Wed, 22 Jul 2020 14:06:06: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:06:14: 26000000 INFO @ Wed, 22 Jul 2020 14:06:14: 22000000 INFO @ Wed, 22 Jul 2020 14:06:15: 22000000 INFO @ Wed, 22 Jul 2020 14:06:22: 27000000 INFO @ Wed, 22 Jul 2020 14:06:22: 23000000 INFO @ Wed, 22 Jul 2020 14:06:23: 23000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:06:30: 28000000 INFO @ Wed, 22 Jul 2020 14:06:30: 24000000 INFO @ Wed, 22 Jul 2020 14:06:31: 24000000 INFO @ Wed, 22 Jul 2020 14:06:38: 29000000 INFO @ Wed, 22 Jul 2020 14:06:39: 25000000 INFO @ Wed, 22 Jul 2020 14:06:40: 25000000 INFO @ Wed, 22 Jul 2020 14:06:47: 30000000 INFO @ Wed, 22 Jul 2020 14:06:47: 26000000 INFO @ Wed, 22 Jul 2020 14:06:48: 26000000 INFO @ Wed, 22 Jul 2020 14:06:55: 31000000 INFO @ Wed, 22 Jul 2020 14:06:56: 27000000 INFO @ Wed, 22 Jul 2020 14:06:57: 27000000 INFO @ Wed, 22 Jul 2020 14:07:03: 32000000 INFO @ Wed, 22 Jul 2020 14:07:04: 28000000 INFO @ Wed, 22 Jul 2020 14:07:05: 28000000 INFO @ Wed, 22 Jul 2020 14:07:11: 33000000 INFO @ Wed, 22 Jul 2020 14:07:13: 29000000 INFO @ Wed, 22 Jul 2020 14:07:13: 29000000 INFO @ Wed, 22 Jul 2020 14:07:20: 34000000 INFO @ Wed, 22 Jul 2020 14:07:21: 30000000 INFO @ Wed, 22 Jul 2020 14:07:22: 30000000 INFO @ Wed, 22 Jul 2020 14:07:28: 35000000 INFO @ Wed, 22 Jul 2020 14:07:29: 31000000 INFO @ Wed, 22 Jul 2020 14:07:30: 31000000 INFO @ Wed, 22 Jul 2020 14:07:37: 36000000 INFO @ Wed, 22 Jul 2020 14:07:38: 32000000 INFO @ Wed, 22 Jul 2020 14:07:39: 32000000 INFO @ Wed, 22 Jul 2020 14:07:45: 37000000 INFO @ Wed, 22 Jul 2020 14:07:47: 33000000 INFO @ Wed, 22 Jul 2020 14:07:48: 33000000 INFO @ Wed, 22 Jul 2020 14:07:53: 38000000 INFO @ Wed, 22 Jul 2020 14:07:56: 34000000 INFO @ Wed, 22 Jul 2020 14:07:56: 34000000 INFO @ Wed, 22 Jul 2020 14:08:02: 39000000 INFO @ Wed, 22 Jul 2020 14:08:04: 35000000 INFO @ Wed, 22 Jul 2020 14:08:05: 35000000 INFO @ Wed, 22 Jul 2020 14:08:08: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:08:08: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:08:08: #1 total tags in treatment: 16703503 INFO @ Wed, 22 Jul 2020 14:08:08: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:08:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:08:08: #1 tags after filtering in treatment: 10716431 INFO @ Wed, 22 Jul 2020 14:08:08: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 14:08:08: #1 finished! INFO @ Wed, 22 Jul 2020 14:08:08: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:08:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:08:08: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:08:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:08:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:08:12: 36000000 INFO @ Wed, 22 Jul 2020 14:08:13: 36000000 INFO @ Wed, 22 Jul 2020 14:08:20: 37000000 INFO @ Wed, 22 Jul 2020 14:08:21: 37000000 INFO @ Wed, 22 Jul 2020 14:08:29: 38000000 INFO @ Wed, 22 Jul 2020 14:08:29: 38000000 INFO @ Wed, 22 Jul 2020 14:08:37: 39000000 INFO @ Wed, 22 Jul 2020 14:08:38: 39000000 INFO @ Wed, 22 Jul 2020 14:08:43: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:08:43: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:08:43: #1 total tags in treatment: 16703503 INFO @ Wed, 22 Jul 2020 14:08:43: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:08:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:08:43: #1 tags after filtering in treatment: 10716431 INFO @ Wed, 22 Jul 2020 14:08:43: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 14:08:43: #1 finished! INFO @ Wed, 22 Jul 2020 14:08:43: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:08:43: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:08:44: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:08:44: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:08:44: #1 total tags in treatment: 16703503 INFO @ Wed, 22 Jul 2020 14:08:44: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:08:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:08:44: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:08:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:08:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:08:44: #1 tags after filtering in treatment: 10716431 INFO @ Wed, 22 Jul 2020 14:08:44: #1 Redundant rate of treatment: 0.36 INFO @ Wed, 22 Jul 2020 14:08:44: #1 finished! INFO @ Wed, 22 Jul 2020 14:08:44: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:08:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:08:45: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:08:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:08:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217638/SRX7217638.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling