Job ID = 7107801 SRX = SRX7217637 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21410821 spots for SRR10533714/SRR10533714.sra Written 21410821 spots for SRR10533714/SRR10533714.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:12 21410821 reads; of these: 21410821 (100.00%) were paired; of these: 9506839 (44.40%) aligned concordantly 0 times 9344810 (43.65%) aligned concordantly exactly 1 time 2559172 (11.95%) aligned concordantly >1 times ---- 9506839 pairs aligned concordantly 0 times; of these: 3489379 (36.70%) aligned discordantly 1 time ---- 6017460 pairs aligned 0 times concordantly or discordantly; of these: 12034920 mates make up the pairs; of these: 9196587 (76.42%) aligned 0 times 567548 (4.72%) aligned exactly 1 time 2270785 (18.87%) aligned >1 times 78.52% overall alignment rate Time searching: 00:27:12 Overall time: 00:27:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2657944 / 15248730 = 0.1743 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:54:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:54:23: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:54:23: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:54:34: 1000000 INFO @ Wed, 22 Jul 2020 13:54:45: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:54:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:54:53: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:54:53: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:54:57: 3000000 INFO @ Wed, 22 Jul 2020 13:55:05: 1000000 INFO @ Wed, 22 Jul 2020 13:55:10: 4000000 INFO @ Wed, 22 Jul 2020 13:55:17: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:55:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:55:23: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:55:23: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:55:23: 5000000 INFO @ Wed, 22 Jul 2020 13:55:30: 3000000 INFO @ Wed, 22 Jul 2020 13:55:35: 1000000 INFO @ Wed, 22 Jul 2020 13:55:36: 6000000 INFO @ Wed, 22 Jul 2020 13:55:42: 4000000 INFO @ Wed, 22 Jul 2020 13:55:48: 2000000 INFO @ Wed, 22 Jul 2020 13:55:49: 7000000 INFO @ Wed, 22 Jul 2020 13:55:55: 5000000 INFO @ Wed, 22 Jul 2020 13:56:01: 3000000 INFO @ Wed, 22 Jul 2020 13:56:02: 8000000 INFO @ Wed, 22 Jul 2020 13:56:08: 6000000 INFO @ Wed, 22 Jul 2020 13:56:13: 4000000 INFO @ Wed, 22 Jul 2020 13:56:15: 9000000 INFO @ Wed, 22 Jul 2020 13:56:20: 7000000 INFO @ Wed, 22 Jul 2020 13:56:26: 5000000 INFO @ Wed, 22 Jul 2020 13:56:28: 10000000 INFO @ Wed, 22 Jul 2020 13:56:33: 8000000 INFO @ Wed, 22 Jul 2020 13:56:38: 6000000 INFO @ Wed, 22 Jul 2020 13:56:41: 11000000 INFO @ Wed, 22 Jul 2020 13:56:45: 9000000 INFO @ Wed, 22 Jul 2020 13:56:51: 7000000 INFO @ Wed, 22 Jul 2020 13:56:54: 12000000 INFO @ Wed, 22 Jul 2020 13:56:58: 10000000 INFO @ Wed, 22 Jul 2020 13:57:03: 8000000 INFO @ Wed, 22 Jul 2020 13:57:07: 13000000 INFO @ Wed, 22 Jul 2020 13:57:10: 11000000 INFO @ Wed, 22 Jul 2020 13:57:16: 9000000 INFO @ Wed, 22 Jul 2020 13:57:20: 14000000 INFO @ Wed, 22 Jul 2020 13:57:23: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:57:28: 10000000 INFO @ Wed, 22 Jul 2020 13:57:33: 15000000 INFO @ Wed, 22 Jul 2020 13:57:35: 13000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:57:40: 11000000 INFO @ Wed, 22 Jul 2020 13:57:46: 16000000 INFO @ Wed, 22 Jul 2020 13:57:47: 14000000 INFO @ Wed, 22 Jul 2020 13:57:52: 12000000 INFO @ Wed, 22 Jul 2020 13:57:59: 17000000 INFO @ Wed, 22 Jul 2020 13:58:00: 15000000 INFO @ Wed, 22 Jul 2020 13:58:04: 13000000 INFO @ Wed, 22 Jul 2020 13:58:12: 16000000 INFO @ Wed, 22 Jul 2020 13:58:13: 18000000 INFO @ Wed, 22 Jul 2020 13:58:17: 14000000 INFO @ Wed, 22 Jul 2020 13:58:24: 17000000 INFO @ Wed, 22 Jul 2020 13:58:26: 19000000 INFO @ Wed, 22 Jul 2020 13:58:29: 15000000 INFO @ Wed, 22 Jul 2020 13:58:37: 18000000 INFO @ Wed, 22 Jul 2020 13:58:38: 20000000 INFO @ Wed, 22 Jul 2020 13:58:41: 16000000 INFO @ Wed, 22 Jul 2020 13:58:49: 19000000 INFO @ Wed, 22 Jul 2020 13:58:51: 21000000 INFO @ Wed, 22 Jul 2020 13:58:54: 17000000 INFO @ Wed, 22 Jul 2020 13:59:01: 20000000 INFO @ Wed, 22 Jul 2020 13:59:04: 22000000 INFO @ Wed, 22 Jul 2020 13:59:06: 18000000 INFO @ Wed, 22 Jul 2020 13:59:14: 21000000 INFO @ Wed, 22 Jul 2020 13:59:17: 23000000 INFO @ Wed, 22 Jul 2020 13:59:18: 19000000 INFO @ Wed, 22 Jul 2020 13:59:26: 22000000 INFO @ Wed, 22 Jul 2020 13:59:31: 24000000 INFO @ Wed, 22 Jul 2020 13:59:31: 20000000 INFO @ Wed, 22 Jul 2020 13:59:39: 23000000 INFO @ Wed, 22 Jul 2020 13:59:43: 21000000 INFO @ Wed, 22 Jul 2020 13:59:44: 25000000 INFO @ Wed, 22 Jul 2020 13:59:51: 24000000 INFO @ Wed, 22 Jul 2020 13:59:55: 22000000 INFO @ Wed, 22 Jul 2020 13:59:57: 26000000 INFO @ Wed, 22 Jul 2020 14:00:04: 25000000 INFO @ Wed, 22 Jul 2020 14:00:08: 23000000 INFO @ Wed, 22 Jul 2020 14:00:10: 27000000 INFO @ Wed, 22 Jul 2020 14:00:16: 26000000 INFO @ Wed, 22 Jul 2020 14:00:20: 24000000 INFO @ Wed, 22 Jul 2020 14:00:23: 28000000 INFO @ Wed, 22 Jul 2020 14:00:27: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:00:27: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:00:27: #1 total tags in treatment: 9650918 INFO @ Wed, 22 Jul 2020 14:00:27: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:00:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:00:27: #1 tags after filtering in treatment: 6424935 INFO @ Wed, 22 Jul 2020 14:00:27: #1 Redundant rate of treatment: 0.33 INFO @ Wed, 22 Jul 2020 14:00:27: #1 finished! INFO @ Wed, 22 Jul 2020 14:00:27: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:00:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:00:28: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:00:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:00:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:00:29: 27000000 INFO @ Wed, 22 Jul 2020 14:00:33: 25000000 INFO @ Wed, 22 Jul 2020 14:00:41: 28000000 INFO @ Wed, 22 Jul 2020 14:00:44: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:00:44: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:00:44: #1 total tags in treatment: 9650918 INFO @ Wed, 22 Jul 2020 14:00:44: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:00:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:00:44: 26000000 INFO @ Wed, 22 Jul 2020 14:00:44: #1 tags after filtering in treatment: 6424935 INFO @ Wed, 22 Jul 2020 14:00:44: #1 Redundant rate of treatment: 0.33 INFO @ Wed, 22 Jul 2020 14:00:44: #1 finished! INFO @ Wed, 22 Jul 2020 14:00:44: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:00:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:00:45: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:00:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:00:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:00:55: 27000000 INFO @ Wed, 22 Jul 2020 14:01:06: 28000000 INFO @ Wed, 22 Jul 2020 14:01:09: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:01:09: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:01:09: #1 total tags in treatment: 9650918 INFO @ Wed, 22 Jul 2020 14:01:09: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:01:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:01:09: #1 tags after filtering in treatment: 6424935 INFO @ Wed, 22 Jul 2020 14:01:09: #1 Redundant rate of treatment: 0.33 INFO @ Wed, 22 Jul 2020 14:01:09: #1 finished! INFO @ Wed, 22 Jul 2020 14:01:09: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:01:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:01:10: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:01:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:01:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7217637/SRX7217637.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling