Job ID = 7107730 SRX = SRX717556 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 4923394 spots for SRR1593251/SRR1593251.sra Written 4923394 spots for SRR1593251/SRR1593251.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:55 4923394 reads; of these: 4923394 (100.00%) were unpaired; of these: 218891 (4.45%) aligned 0 times 3840675 (78.01%) aligned exactly 1 time 863828 (17.55%) aligned >1 times 95.55% overall alignment rate Time searching: 00:00:55 Overall time: 00:00:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1897553 / 4704503 = 0.4033 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:14:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:14:20: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:14:20: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:14:26: 1000000 INFO @ Wed, 22 Jul 2020 13:14:31: 2000000 INFO @ Wed, 22 Jul 2020 13:14:36: #1 tag size is determined as 60 bps INFO @ Wed, 22 Jul 2020 13:14:36: #1 tag size = 60 INFO @ Wed, 22 Jul 2020 13:14:36: #1 total tags in treatment: 2806950 INFO @ Wed, 22 Jul 2020 13:14:36: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:14:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:14:36: #1 tags after filtering in treatment: 2806950 INFO @ Wed, 22 Jul 2020 13:14:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:14:36: #1 finished! INFO @ Wed, 22 Jul 2020 13:14:36: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:14:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:14:36: #2 number of paired peaks: 30 WARNING @ Wed, 22 Jul 2020 13:14:36: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:14:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:14:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:14:50: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:14:50: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:14:56: 1000000 INFO @ Wed, 22 Jul 2020 13:15:01: 2000000 INFO @ Wed, 22 Jul 2020 13:15:06: #1 tag size is determined as 60 bps INFO @ Wed, 22 Jul 2020 13:15:06: #1 tag size = 60 INFO @ Wed, 22 Jul 2020 13:15:06: #1 total tags in treatment: 2806950 INFO @ Wed, 22 Jul 2020 13:15:06: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:15:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:15:06: #1 tags after filtering in treatment: 2806950 INFO @ Wed, 22 Jul 2020 13:15:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:15:06: #1 finished! INFO @ Wed, 22 Jul 2020 13:15:06: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:15:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:15:06: #2 number of paired peaks: 30 WARNING @ Wed, 22 Jul 2020 13:15:06: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:15:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:15:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:15:20: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:15:20: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:15:26: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:15:31: 2000000 INFO @ Wed, 22 Jul 2020 13:15:36: #1 tag size is determined as 60 bps INFO @ Wed, 22 Jul 2020 13:15:36: #1 tag size = 60 INFO @ Wed, 22 Jul 2020 13:15:36: #1 total tags in treatment: 2806950 INFO @ Wed, 22 Jul 2020 13:15:36: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:15:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:15:36: #1 tags after filtering in treatment: 2806950 INFO @ Wed, 22 Jul 2020 13:15:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:15:36: #1 finished! INFO @ Wed, 22 Jul 2020 13:15:36: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:15:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:15:36: #2 number of paired peaks: 30 WARNING @ Wed, 22 Jul 2020 13:15:36: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:15:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717556/SRX717556.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。