Job ID = 5791225 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 70,341 reads read : 70,341 reads written : 70,341 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1593248.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 70341 reads; of these: 70341 (100.00%) were unpaired; of these: 31538 (44.84%) aligned 0 times 31631 (44.97%) aligned exactly 1 time 7172 (10.20%) aligned >1 times 55.16% overall alignment rate Time searching: 00:00:00 Overall time: 00:00:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1396 / 38803 = 0.0360 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:05:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:05:27: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:05:27: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:05:27: #1 tag size is determined as 60 bps INFO @ Wed, 22 Apr 2020 09:05:27: #1 tag size = 60 INFO @ Wed, 22 Apr 2020 09:05:27: #1 total tags in treatment: 37407 INFO @ Wed, 22 Apr 2020 09:05:27: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:05:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:05:27: #1 tags after filtering in treatment: 37406 INFO @ Wed, 22 Apr 2020 09:05:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 09:05:27: #1 finished! INFO @ Wed, 22 Apr 2020 09:05:27: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:05:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:05:27: #2 number of paired peaks: 4 WARNING @ Wed, 22 Apr 2020 09:05:27: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:05:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 09:05:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:05:57: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:05:57: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:05:57: #1 tag size is determined as 60 bps INFO @ Wed, 22 Apr 2020 09:05:57: #1 tag size = 60 INFO @ Wed, 22 Apr 2020 09:05:57: #1 total tags in treatment: 37407 INFO @ Wed, 22 Apr 2020 09:05:57: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:05:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:05:57: #1 tags after filtering in treatment: 37406 INFO @ Wed, 22 Apr 2020 09:05:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 09:05:57: #1 finished! INFO @ Wed, 22 Apr 2020 09:05:57: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:05:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:05:57: #2 number of paired peaks: 4 WARNING @ Wed, 22 Apr 2020 09:05:57: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:05:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Wed, 22 Apr 2020 09:06:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 09:06:28: #1 read tag files... INFO @ Wed, 22 Apr 2020 09:06:28: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 09:06:28: #1 tag size is determined as 60 bps INFO @ Wed, 22 Apr 2020 09:06:28: #1 tag size = 60 INFO @ Wed, 22 Apr 2020 09:06:28: #1 total tags in treatment: 37407 INFO @ Wed, 22 Apr 2020 09:06:28: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 09:06:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 09:06:28: #1 tags after filtering in treatment: 37406 INFO @ Wed, 22 Apr 2020 09:06:28: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 09:06:28: #1 finished! INFO @ Wed, 22 Apr 2020 09:06:28: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 09:06:28: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 09:06:28: #2 number of paired peaks: 4 WARNING @ Wed, 22 Apr 2020 09:06:28: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 09:06:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717553/SRX717553.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling