Job ID = 10223931 SRX = SRX717540 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11627021 spots for SRR1593235/SRR1593235.sra Written 11627021 spots for SRR1593235/SRR1593235.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:21 11627021 reads; of these: 11627021 (100.00%) were unpaired; of these: 298165 (2.56%) aligned 0 times 8501090 (73.11%) aligned exactly 1 time 2827766 (24.32%) aligned >1 times 97.44% overall alignment rate Time searching: 00:01:21 Overall time: 00:01:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6846144 / 11328856 = 0.6043 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:54:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:54:03: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:54:03: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:54:08: 1000000 INFO @ Fri, 16 Oct 2020 08:54:13: 2000000 INFO @ Fri, 16 Oct 2020 08:54:18: 3000000 INFO @ Fri, 16 Oct 2020 08:54:24: 4000000 INFO @ Fri, 16 Oct 2020 08:54:27: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 08:54:27: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 08:54:27: #1 total tags in treatment: 4482712 INFO @ Fri, 16 Oct 2020 08:54:27: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:54:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:54:27: #1 tags after filtering in treatment: 4482712 INFO @ Fri, 16 Oct 2020 08:54:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 08:54:27: #1 finished! INFO @ Fri, 16 Oct 2020 08:54:27: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:54:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:54:27: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:54:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:54:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:54:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:54:33: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:54:33: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:54:40: 1000000 INFO @ Fri, 16 Oct 2020 08:54:47: 2000000 INFO @ Fri, 16 Oct 2020 08:54:53: 3000000 INFO @ Fri, 16 Oct 2020 08:55:01: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:55:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:55:03: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:55:03: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:55:04: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 08:55:04: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 08:55:04: #1 total tags in treatment: 4482712 INFO @ Fri, 16 Oct 2020 08:55:04: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:55:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:55:04: #1 tags after filtering in treatment: 4482712 INFO @ Fri, 16 Oct 2020 08:55:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 08:55:04: #1 finished! INFO @ Fri, 16 Oct 2020 08:55:04: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:55:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:55:04: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:55:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:55:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:55:09: 1000000 INFO @ Fri, 16 Oct 2020 08:55:15: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:55:21: 3000000 INFO @ Fri, 16 Oct 2020 08:55:27: 4000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 08:55:30: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 08:55:30: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 08:55:30: #1 total tags in treatment: 4482712 INFO @ Fri, 16 Oct 2020 08:55:30: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:55:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:55:30: #1 tags after filtering in treatment: 4482712 INFO @ Fri, 16 Oct 2020 08:55:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 08:55:30: #1 finished! INFO @ Fri, 16 Oct 2020 08:55:30: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:55:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:55:31: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:55:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:55:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717540/SRX717540.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling