Job ID = 10223928 SRX = SRX717536 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8375686 spots for SRR1593231/SRR1593231.sra Written 8375686 spots for SRR1593231/SRR1593231.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:51 8375686 reads; of these: 8375686 (100.00%) were unpaired; of these: 532892 (6.36%) aligned 0 times 6501209 (77.62%) aligned exactly 1 time 1341585 (16.02%) aligned >1 times 93.64% overall alignment rate Time searching: 00:00:51 Overall time: 00:00:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4064534 / 7842794 = 0.5183 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:52:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:52:14: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:52:14: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:52:19: 1000000 INFO @ Fri, 16 Oct 2020 08:52:24: 2000000 INFO @ Fri, 16 Oct 2020 08:52:29: 3000000 INFO @ Fri, 16 Oct 2020 08:52:33: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 08:52:33: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 08:52:33: #1 total tags in treatment: 3778260 INFO @ Fri, 16 Oct 2020 08:52:33: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:52:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:52:33: #1 tags after filtering in treatment: 3778260 INFO @ Fri, 16 Oct 2020 08:52:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 08:52:33: #1 finished! INFO @ Fri, 16 Oct 2020 08:52:33: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:52:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:52:34: #2 number of paired peaks: 3 WARNING @ Fri, 16 Oct 2020 08:52:34: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:52:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:52:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:52:44: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:52:44: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:52:49: 1000000 INFO @ Fri, 16 Oct 2020 08:52:54: 2000000 INFO @ Fri, 16 Oct 2020 08:53:00: 3000000 INFO @ Fri, 16 Oct 2020 08:53:04: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 08:53:04: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 08:53:04: #1 total tags in treatment: 3778260 INFO @ Fri, 16 Oct 2020 08:53:04: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:53:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:53:04: #1 tags after filtering in treatment: 3778260 INFO @ Fri, 16 Oct 2020 08:53:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 08:53:04: #1 finished! INFO @ Fri, 16 Oct 2020 08:53:04: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:53:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:53:04: #2 number of paired peaks: 3 WARNING @ Fri, 16 Oct 2020 08:53:04: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:53:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:53:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:53:14: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:53:14: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:53:19: 1000000 INFO @ Fri, 16 Oct 2020 08:53:24: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:53:29: 3000000 INFO @ Fri, 16 Oct 2020 08:53:33: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 08:53:33: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 08:53:33: #1 total tags in treatment: 3778260 INFO @ Fri, 16 Oct 2020 08:53:33: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:53:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:53:33: #1 tags after filtering in treatment: 3778260 INFO @ Fri, 16 Oct 2020 08:53:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 08:53:33: #1 finished! INFO @ Fri, 16 Oct 2020 08:53:33: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:53:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:53:34: #2 number of paired peaks: 3 WARNING @ Fri, 16 Oct 2020 08:53:34: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:53:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717536/SRX717536.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。