Job ID = 7107662 SRX = SRX717498 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5299215 spots for SRR1593193/SRR1593193.sra Written 5299215 spots for SRR1593193/SRR1593193.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:53 5299215 reads; of these: 5299215 (100.00%) were unpaired; of these: 451643 (8.52%) aligned 0 times 4420039 (83.41%) aligned exactly 1 time 427533 (8.07%) aligned >1 times 91.48% overall alignment rate Time searching: 00:00:53 Overall time: 00:00:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2021033 / 4847572 = 0.4169 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:11:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:11:36: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:11:36: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:11:42: 1000000 INFO @ Wed, 22 Jul 2020 13:11:47: 2000000 INFO @ Wed, 22 Jul 2020 13:11:51: #1 tag size is determined as 60 bps INFO @ Wed, 22 Jul 2020 13:11:51: #1 tag size = 60 INFO @ Wed, 22 Jul 2020 13:11:51: #1 total tags in treatment: 2826539 INFO @ Wed, 22 Jul 2020 13:11:51: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:11:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:11:51: #1 tags after filtering in treatment: 2826539 INFO @ Wed, 22 Jul 2020 13:11:51: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:11:51: #1 finished! INFO @ Wed, 22 Jul 2020 13:11:51: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:11:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:11:51: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:11:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:11:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:12:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:12:06: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:12:06: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:12:12: 1000000 INFO @ Wed, 22 Jul 2020 13:12:17: 2000000 INFO @ Wed, 22 Jul 2020 13:12:21: #1 tag size is determined as 60 bps INFO @ Wed, 22 Jul 2020 13:12:21: #1 tag size = 60 INFO @ Wed, 22 Jul 2020 13:12:21: #1 total tags in treatment: 2826539 INFO @ Wed, 22 Jul 2020 13:12:21: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:12:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:12:21: #1 tags after filtering in treatment: 2826539 INFO @ Wed, 22 Jul 2020 13:12:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:12:21: #1 finished! INFO @ Wed, 22 Jul 2020 13:12:21: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:12:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:12:21: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:12:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:12:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:12:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:12:36: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:12:36: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:12:42: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:12:48: 2000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:12:53: #1 tag size is determined as 60 bps INFO @ Wed, 22 Jul 2020 13:12:53: #1 tag size = 60 INFO @ Wed, 22 Jul 2020 13:12:53: #1 total tags in treatment: 2826539 INFO @ Wed, 22 Jul 2020 13:12:53: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:12:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:12:53: #1 tags after filtering in treatment: 2826539 INFO @ Wed, 22 Jul 2020 13:12:53: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:12:53: #1 finished! INFO @ Wed, 22 Jul 2020 13:12:53: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:12:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:12:53: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:12:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:12:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717498/SRX717498.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling