Job ID = 10223927 SRX = SRX717435 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8702653 spots for SRR1593130/SRR1593130.sra Written 8702653 spots for SRR1593130/SRR1593130.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:00 8702653 reads; of these: 8702653 (100.00%) were unpaired; of these: 678880 (7.80%) aligned 0 times 7596522 (87.29%) aligned exactly 1 time 427251 (4.91%) aligned >1 times 92.20% overall alignment rate Time searching: 00:01:00 Overall time: 00:01:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4437021 / 8023773 = 0.5530 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:52:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:52:41: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:52:41: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:52:47: 1000000 INFO @ Fri, 16 Oct 2020 08:52:53: 2000000 INFO @ Fri, 16 Oct 2020 08:52:59: 3000000 INFO @ Fri, 16 Oct 2020 08:53:02: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 08:53:02: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 08:53:02: #1 total tags in treatment: 3586752 INFO @ Fri, 16 Oct 2020 08:53:02: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:53:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:53:02: #1 tags after filtering in treatment: 3586752 INFO @ Fri, 16 Oct 2020 08:53:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 08:53:02: #1 finished! INFO @ Fri, 16 Oct 2020 08:53:02: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:53:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:53:02: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:53:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:53:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:53:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:53:11: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:53:11: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:53:17: 1000000 INFO @ Fri, 16 Oct 2020 08:53:23: 2000000 INFO @ Fri, 16 Oct 2020 08:53:29: 3000000 INFO @ Fri, 16 Oct 2020 08:53:32: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 08:53:32: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 08:53:32: #1 total tags in treatment: 3586752 INFO @ Fri, 16 Oct 2020 08:53:32: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:53:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:53:32: #1 tags after filtering in treatment: 3586752 INFO @ Fri, 16 Oct 2020 08:53:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 08:53:32: #1 finished! INFO @ Fri, 16 Oct 2020 08:53:32: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:53:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:53:32: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:53:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:53:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:53:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:53:41: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:53:41: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:53:46: 1000000 INFO @ Fri, 16 Oct 2020 08:53:50: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:53:55: 3000000 INFO @ Fri, 16 Oct 2020 08:53:58: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 08:53:58: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 08:53:58: #1 total tags in treatment: 3586752 INFO @ Fri, 16 Oct 2020 08:53:58: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:53:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:53:58: #1 tags after filtering in treatment: 3586752 INFO @ Fri, 16 Oct 2020 08:53:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 08:53:58: #1 finished! INFO @ Fri, 16 Oct 2020 08:53:58: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:53:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:53:58: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:53:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:53:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX717435/SRX717435.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。