Job ID = 14520726 SRX = SRX7119866 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 23362888 spots for SRR10423773/SRR10423773.sra Written 23362888 spots for SRR10423773/SRR10423773.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:10 23362888 reads; of these: 23362888 (100.00%) were unpaired; of these: 3354487 (14.36%) aligned 0 times 18503223 (79.20%) aligned exactly 1 time 1505178 (6.44%) aligned >1 times 85.64% overall alignment rate Time searching: 00:03:10 Overall time: 00:03:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13936362 / 20008401 = 0.6965 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:36: 1000000 INFO @ Sat, 15 Jan 2022 19:49:41: 2000000 INFO @ Sat, 15 Jan 2022 19:49:46: 3000000 INFO @ Sat, 15 Jan 2022 19:49:51: 4000000 INFO @ Sat, 15 Jan 2022 19:49:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:01: 6000000 INFO @ Sat, 15 Jan 2022 19:50:01: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:50:01: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:50:01: #1 total tags in treatment: 6072039 INFO @ Sat, 15 Jan 2022 19:50:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:01: #1 tags after filtering in treatment: 6072039 INFO @ Sat, 15 Jan 2022 19:50:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:50:01: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:50:08: 1000000 INFO @ Sat, 15 Jan 2022 19:50:14: 2000000 INFO @ Sat, 15 Jan 2022 19:50:20: 3000000 INFO @ Sat, 15 Jan 2022 19:50:26: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:32: 5000000 INFO @ Sat, 15 Jan 2022 19:50:37: 1000000 INFO @ Sat, 15 Jan 2022 19:50:38: 6000000 INFO @ Sat, 15 Jan 2022 19:50:39: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:50:39: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:50:39: #1 total tags in treatment: 6072039 INFO @ Sat, 15 Jan 2022 19:50:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:39: #1 tags after filtering in treatment: 6072039 INFO @ Sat, 15 Jan 2022 19:50:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:50:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:50:43: 2000000 INFO @ Sat, 15 Jan 2022 19:50:48: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:50:53: 4000000 INFO @ Sat, 15 Jan 2022 19:50:58: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:51:03: 6000000 INFO @ Sat, 15 Jan 2022 19:51:03: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:51:03: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:51:03: #1 total tags in treatment: 6072039 INFO @ Sat, 15 Jan 2022 19:51:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:03: #1 tags after filtering in treatment: 6072039 INFO @ Sat, 15 Jan 2022 19:51:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:03: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119866/SRX7119866.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling