Job ID = 14520722 SRX = SRX7119862 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21839761 spots for SRR10423769/SRR10423769.sra Written 21839761 spots for SRR10423769/SRR10423769.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:21 21839761 reads; of these: 21839761 (100.00%) were unpaired; of these: 1444154 (6.61%) aligned 0 times 18268977 (83.65%) aligned exactly 1 time 2126630 (9.74%) aligned >1 times 93.39% overall alignment rate Time searching: 00:03:21 Overall time: 00:03:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8838244 / 20395607 = 0.4333 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:09: 1000000 INFO @ Sat, 15 Jan 2022 19:50:15: 2000000 INFO @ Sat, 15 Jan 2022 19:50:20: 3000000 INFO @ Sat, 15 Jan 2022 19:50:26: 4000000 INFO @ Sat, 15 Jan 2022 19:50:31: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:37: 6000000 INFO @ Sat, 15 Jan 2022 19:50:39: 1000000 INFO @ Sat, 15 Jan 2022 19:50:42: 7000000 INFO @ Sat, 15 Jan 2022 19:50:45: 2000000 INFO @ Sat, 15 Jan 2022 19:50:48: 8000000 INFO @ Sat, 15 Jan 2022 19:50:50: 3000000 INFO @ Sat, 15 Jan 2022 19:50:54: 9000000 INFO @ Sat, 15 Jan 2022 19:50:56: 4000000 INFO @ Sat, 15 Jan 2022 19:50:59: 10000000 BedGraph に変換中... INFO @ Sat, 15 Jan 2022 19:51:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:51:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:51:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:51:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:51:05: 11000000 INFO @ Sat, 15 Jan 2022 19:51:07: 6000000 INFO @ Sat, 15 Jan 2022 19:51:08: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:51:08: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:51:08: #1 total tags in treatment: 11557363 INFO @ Sat, 15 Jan 2022 19:51:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:09: 1000000 INFO @ Sat, 15 Jan 2022 19:51:09: #1 tags after filtering in treatment: 11557363 INFO @ Sat, 15 Jan 2022 19:51:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:09: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:51:12: 7000000 INFO @ Sat, 15 Jan 2022 19:51:14: 2000000 INFO @ Sat, 15 Jan 2022 19:51:18: 8000000 INFO @ Sat, 15 Jan 2022 19:51:19: 3000000 INFO @ Sat, 15 Jan 2022 19:51:23: 4000000 INFO @ Sat, 15 Jan 2022 19:51:24: 9000000 INFO @ Sat, 15 Jan 2022 19:51:28: 5000000 INFO @ Sat, 15 Jan 2022 19:51:29: 10000000 INFO @ Sat, 15 Jan 2022 19:51:33: 6000000 INFO @ Sat, 15 Jan 2022 19:51:35: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:51:38: 7000000 INFO @ Sat, 15 Jan 2022 19:51:38: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:51:38: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:51:38: #1 total tags in treatment: 11557363 INFO @ Sat, 15 Jan 2022 19:51:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:39: #1 tags after filtering in treatment: 11557363 INFO @ Sat, 15 Jan 2022 19:51:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:51:43: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:51:48: 9000000 INFO @ Sat, 15 Jan 2022 19:51:52: 10000000 INFO @ Sat, 15 Jan 2022 19:51:57: 11000000 INFO @ Sat, 15 Jan 2022 19:52:00: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:52:00: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:52:00: #1 total tags in treatment: 11557363 INFO @ Sat, 15 Jan 2022 19:52:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:52:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:52:00: #1 tags after filtering in treatment: 11557363 INFO @ Sat, 15 Jan 2022 19:52:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:52:00: #1 finished! INFO @ Sat, 15 Jan 2022 19:52:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:52:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:52:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:52:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:52:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119862/SRX7119862.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling