Job ID = 14520712 SRX = SRX7119849 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19762905 spots for SRR10423756/SRR10423756.sra Written 19762905 spots for SRR10423756/SRR10423756.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:14 19762905 reads; of these: 19762905 (100.00%) were unpaired; of these: 1101389 (5.57%) aligned 0 times 17466874 (88.38%) aligned exactly 1 time 1194642 (6.04%) aligned >1 times 94.43% overall alignment rate Time searching: 00:03:14 Overall time: 00:03:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8720046 / 18661516 = 0.4673 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:22: 1000000 INFO @ Sat, 15 Jan 2022 19:48:27: 2000000 INFO @ Sat, 15 Jan 2022 19:48:33: 3000000 INFO @ Sat, 15 Jan 2022 19:48:38: 4000000 INFO @ Sat, 15 Jan 2022 19:48:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:48: 6000000 INFO @ Sat, 15 Jan 2022 19:48:53: 1000000 INFO @ Sat, 15 Jan 2022 19:48:53: 7000000 INFO @ Sat, 15 Jan 2022 19:48:58: 2000000 INFO @ Sat, 15 Jan 2022 19:48:59: 8000000 INFO @ Sat, 15 Jan 2022 19:49:04: 3000000 INFO @ Sat, 15 Jan 2022 19:49:04: 9000000 INFO @ Sat, 15 Jan 2022 19:49:09: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:49:09: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:49:09: #1 total tags in treatment: 9941470 INFO @ Sat, 15 Jan 2022 19:49:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:09: 4000000 INFO @ Sat, 15 Jan 2022 19:49:09: #1 tags after filtering in treatment: 9941470 INFO @ Sat, 15 Jan 2022 19:49:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:49:09: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:49:15: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:20: 6000000 INFO @ Sat, 15 Jan 2022 19:49:23: 1000000 INFO @ Sat, 15 Jan 2022 19:49:26: 7000000 INFO @ Sat, 15 Jan 2022 19:49:29: 2000000 INFO @ Sat, 15 Jan 2022 19:49:32: 8000000 INFO @ Sat, 15 Jan 2022 19:49:35: 3000000 INFO @ Sat, 15 Jan 2022 19:49:37: 9000000 INFO @ Sat, 15 Jan 2022 19:49:41: 4000000 INFO @ Sat, 15 Jan 2022 19:49:43: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:49:43: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:49:43: #1 total tags in treatment: 9941470 INFO @ Sat, 15 Jan 2022 19:49:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:43: #1 tags after filtering in treatment: 9941470 INFO @ Sat, 15 Jan 2022 19:49:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:49:43: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:49:46: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:49:52: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:49:58: 7000000 INFO @ Sat, 15 Jan 2022 19:50:03: 8000000 INFO @ Sat, 15 Jan 2022 19:50:08: 9000000 INFO @ Sat, 15 Jan 2022 19:50:14: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:50:14: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:50:14: #1 total tags in treatment: 9941470 INFO @ Sat, 15 Jan 2022 19:50:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:14: #1 tags after filtering in treatment: 9941470 INFO @ Sat, 15 Jan 2022 19:50:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:50:14: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119849/SRX7119849.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling