Job ID = 14520711 SRX = SRX7119848 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24274825 spots for SRR10423755/SRR10423755.sra Written 24274825 spots for SRR10423755/SRR10423755.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:30 24274825 reads; of these: 24274825 (100.00%) were unpaired; of these: 1338695 (5.51%) aligned 0 times 21377271 (88.06%) aligned exactly 1 time 1558859 (6.42%) aligned >1 times 94.49% overall alignment rate Time searching: 00:03:30 Overall time: 00:03:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11747691 / 22936130 = 0.5122 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:32: 1000000 INFO @ Sat, 15 Jan 2022 19:49:37: 2000000 INFO @ Sat, 15 Jan 2022 19:49:42: 3000000 INFO @ Sat, 15 Jan 2022 19:49:47: 4000000 INFO @ Sat, 15 Jan 2022 19:49:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:57: 6000000 INFO @ Sat, 15 Jan 2022 19:49:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:02: 7000000 INFO @ Sat, 15 Jan 2022 19:50:04: 1000000 INFO @ Sat, 15 Jan 2022 19:50:08: 8000000 INFO @ Sat, 15 Jan 2022 19:50:10: 2000000 INFO @ Sat, 15 Jan 2022 19:50:13: 9000000 INFO @ Sat, 15 Jan 2022 19:50:17: 3000000 INFO @ Sat, 15 Jan 2022 19:50:19: 10000000 INFO @ Sat, 15 Jan 2022 19:50:23: 4000000 INFO @ Sat, 15 Jan 2022 19:50:25: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:26: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:50:26: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:50:26: #1 total tags in treatment: 11188439 INFO @ Sat, 15 Jan 2022 19:50:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:26: #1 tags after filtering in treatment: 11188439 INFO @ Sat, 15 Jan 2022 19:50:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:50:26: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:50:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:30: 5000000 INFO @ Sat, 15 Jan 2022 19:50:33: 1000000 INFO @ Sat, 15 Jan 2022 19:50:36: 6000000 INFO @ Sat, 15 Jan 2022 19:50:39: 2000000 INFO @ Sat, 15 Jan 2022 19:50:42: 7000000 INFO @ Sat, 15 Jan 2022 19:50:44: 3000000 INFO @ Sat, 15 Jan 2022 19:50:49: 8000000 INFO @ Sat, 15 Jan 2022 19:50:50: 4000000 INFO @ Sat, 15 Jan 2022 19:50:55: 9000000 INFO @ Sat, 15 Jan 2022 19:50:56: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:51:01: 10000000 INFO @ Sat, 15 Jan 2022 19:51:02: 6000000 INFO @ Sat, 15 Jan 2022 19:51:07: 7000000 INFO @ Sat, 15 Jan 2022 19:51:07: 11000000 INFO @ Sat, 15 Jan 2022 19:51:08: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:51:08: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:51:08: #1 total tags in treatment: 11188439 INFO @ Sat, 15 Jan 2022 19:51:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:08: #1 tags after filtering in treatment: 11188439 INFO @ Sat, 15 Jan 2022 19:51:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:09: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:51:12: 8000000 INFO @ Sat, 15 Jan 2022 19:51:17: 9000000 INFO @ Sat, 15 Jan 2022 19:51:22: 10000000 INFO @ Sat, 15 Jan 2022 19:51:27: 11000000 INFO @ Sat, 15 Jan 2022 19:51:28: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:51:28: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:51:28: #1 total tags in treatment: 11188439 INFO @ Sat, 15 Jan 2022 19:51:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:28: #1 tags after filtering in treatment: 11188439 INFO @ Sat, 15 Jan 2022 19:51:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:28: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119848/SRX7119848.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling