Job ID = 14520705 SRX = SRX7119842 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10389696 spots for SRR10423749/SRR10423749.sra Written 10389696 spots for SRR10423749/SRR10423749.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:44 10389696 reads; of these: 10389696 (100.00%) were unpaired; of these: 982653 (9.46%) aligned 0 times 8553073 (82.32%) aligned exactly 1 time 853970 (8.22%) aligned >1 times 90.54% overall alignment rate Time searching: 00:03:44 Overall time: 00:03:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3262749 / 9407043 = 0.3468 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:47:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:47:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:47:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:02: 1000000 INFO @ Sat, 15 Jan 2022 19:48:12: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:21: 3000000 INFO @ Sat, 15 Jan 2022 19:48:31: 4000000 INFO @ Sat, 15 Jan 2022 19:48:35: 1000000 INFO @ Sat, 15 Jan 2022 19:48:41: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:49: 2000000 INFO @ Sat, 15 Jan 2022 19:48:51: 6000000 INFO @ Sat, 15 Jan 2022 19:48:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:53: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:48:53: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:48:53: #1 total tags in treatment: 6144294 INFO @ Sat, 15 Jan 2022 19:48:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:48:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:48:53: #1 tags after filtering in treatment: 6144294 INFO @ Sat, 15 Jan 2022 19:48:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:48:53: #1 finished! INFO @ Sat, 15 Jan 2022 19:48:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:48:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:48:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:48:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:48:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:49:02: 1000000 INFO @ Sat, 15 Jan 2022 19:49:03: 3000000 INFO @ Sat, 15 Jan 2022 19:49:13: 2000000 INFO @ Sat, 15 Jan 2022 19:49:16: 4000000 INFO @ Sat, 15 Jan 2022 19:49:23: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:49:30: 5000000 INFO @ Sat, 15 Jan 2022 19:49:34: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:49:44: 6000000 INFO @ Sat, 15 Jan 2022 19:49:45: 5000000 INFO @ Sat, 15 Jan 2022 19:49:46: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:49:46: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:49:46: #1 total tags in treatment: 6144294 INFO @ Sat, 15 Jan 2022 19:49:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:46: #1 tags after filtering in treatment: 6144294 INFO @ Sat, 15 Jan 2022 19:49:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:49:46: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:49:55: 6000000 INFO @ Sat, 15 Jan 2022 19:49:56: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:49:56: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:49:56: #1 total tags in treatment: 6144294 INFO @ Sat, 15 Jan 2022 19:49:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:56: #1 tags after filtering in treatment: 6144294 INFO @ Sat, 15 Jan 2022 19:49:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:49:56: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119842/SRX7119842.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling