Job ID = 14520704 SRX = SRX7119841 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22504712 spots for SRR10423748/SRR10423748.sra Written 22504712 spots for SRR10423748/SRR10423748.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:03 22504712 reads; of these: 22504712 (100.00%) were unpaired; of these: 1318425 (5.86%) aligned 0 times 18734514 (83.25%) aligned exactly 1 time 2451773 (10.89%) aligned >1 times 94.14% overall alignment rate Time searching: 00:04:03 Overall time: 00:04:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8912844 / 21186287 = 0.4207 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:53: 1000000 INFO @ Sat, 15 Jan 2022 19:51:00: 2000000 INFO @ Sat, 15 Jan 2022 19:51:06: 3000000 INFO @ Sat, 15 Jan 2022 19:51:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:51:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:51:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:51:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:51:20: 5000000 INFO @ Sat, 15 Jan 2022 19:51:23: 1000000 INFO @ Sat, 15 Jan 2022 19:51:27: 6000000 INFO @ Sat, 15 Jan 2022 19:51:30: 2000000 INFO @ Sat, 15 Jan 2022 19:51:33: 7000000 INFO @ Sat, 15 Jan 2022 19:51:36: 3000000 INFO @ Sat, 15 Jan 2022 19:51:40: 8000000 INFO @ Sat, 15 Jan 2022 19:51:43: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:51:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:51:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:51:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:51:47: 9000000 INFO @ Sat, 15 Jan 2022 19:51:49: 5000000 INFO @ Sat, 15 Jan 2022 19:51:54: 1000000 INFO @ Sat, 15 Jan 2022 19:51:54: 10000000 INFO @ Sat, 15 Jan 2022 19:51:56: 6000000 INFO @ Sat, 15 Jan 2022 19:52:01: 11000000 INFO @ Sat, 15 Jan 2022 19:52:01: 2000000 INFO @ Sat, 15 Jan 2022 19:52:03: 7000000 INFO @ Sat, 15 Jan 2022 19:52:07: 12000000 INFO @ Sat, 15 Jan 2022 19:52:07: 3000000 INFO @ Sat, 15 Jan 2022 19:52:09: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:52:09: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:52:09: #1 total tags in treatment: 12273443 INFO @ Sat, 15 Jan 2022 19:52:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:52:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:52:09: #1 tags after filtering in treatment: 12273443 INFO @ Sat, 15 Jan 2022 19:52:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:52:09: #1 finished! INFO @ Sat, 15 Jan 2022 19:52:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:52:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:52:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:52:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:52:10: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 19:52:10: 8000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Sat, 15 Jan 2022 19:52:14: 4000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:52:17: 9000000 INFO @ Sat, 15 Jan 2022 19:52:20: 5000000 INFO @ Sat, 15 Jan 2022 19:52:24: 10000000 INFO @ Sat, 15 Jan 2022 19:52:27: 6000000 INFO @ Sat, 15 Jan 2022 19:52:30: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:52:33: 7000000 INFO @ Sat, 15 Jan 2022 19:52:37: 12000000 INFO @ Sat, 15 Jan 2022 19:52:38: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:52:38: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:52:38: #1 total tags in treatment: 12273443 INFO @ Sat, 15 Jan 2022 19:52:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:52:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:52:39: #1 tags after filtering in treatment: 12273443 INFO @ Sat, 15 Jan 2022 19:52:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:52:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:52:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:52:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:52:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:52:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:52:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:52:40: 8000000 INFO @ Sat, 15 Jan 2022 19:52:46: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:52:52: 10000000 INFO @ Sat, 15 Jan 2022 19:52:58: 11000000 INFO @ Sat, 15 Jan 2022 19:53:04: 12000000 INFO @ Sat, 15 Jan 2022 19:53:06: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:53:06: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:53:06: #1 total tags in treatment: 12273443 INFO @ Sat, 15 Jan 2022 19:53:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:53:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:53:06: #1 tags after filtering in treatment: 12273443 INFO @ Sat, 15 Jan 2022 19:53:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:53:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:53:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:53:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:53:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:53:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:53:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119841/SRX7119841.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling