Job ID = 14520703 SRX = SRX7119840 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 29638420 spots for SRR10423747/SRR10423747.sra Written 29638420 spots for SRR10423747/SRR10423747.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:34 29638420 reads; of these: 29638420 (100.00%) were unpaired; of these: 1119521 (3.78%) aligned 0 times 26919636 (90.83%) aligned exactly 1 time 1599263 (5.40%) aligned >1 times 96.22% overall alignment rate Time searching: 00:05:34 Overall time: 00:05:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16868762 / 28518899 = 0.5915 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:54:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:54:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:54:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:54:34: 1000000 INFO @ Sat, 15 Jan 2022 19:54:41: 2000000 INFO @ Sat, 15 Jan 2022 19:54:47: 3000000 INFO @ Sat, 15 Jan 2022 19:54:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:54:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:54:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:54:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:55:00: 5000000 INFO @ Sat, 15 Jan 2022 19:55:06: 6000000 INFO @ Sat, 15 Jan 2022 19:55:06: 1000000 INFO @ Sat, 15 Jan 2022 19:55:14: 7000000 INFO @ Sat, 15 Jan 2022 19:55:15: 2000000 INFO @ Sat, 15 Jan 2022 19:55:21: 8000000 INFO @ Sat, 15 Jan 2022 19:55:22: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:55:28: 9000000 INFO @ Sat, 15 Jan 2022 19:55:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:55:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:55:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:55:29: 4000000 INFO @ Sat, 15 Jan 2022 19:55:34: 10000000 INFO @ Sat, 15 Jan 2022 19:55:35: 1000000 INFO @ Sat, 15 Jan 2022 19:55:37: 5000000 INFO @ Sat, 15 Jan 2022 19:55:41: 11000000 INFO @ Sat, 15 Jan 2022 19:55:42: 2000000 INFO @ Sat, 15 Jan 2022 19:55:44: 6000000 INFO @ Sat, 15 Jan 2022 19:55:45: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:55:45: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:55:45: #1 total tags in treatment: 11650137 INFO @ Sat, 15 Jan 2022 19:55:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:55:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:55:46: #1 tags after filtering in treatment: 11650137 INFO @ Sat, 15 Jan 2022 19:55:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:55:46: #1 finished! INFO @ Sat, 15 Jan 2022 19:55:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:55:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:55:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:55:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:55:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:55:49: 3000000 INFO @ Sat, 15 Jan 2022 19:55:51: 7000000 INFO @ Sat, 15 Jan 2022 19:55:56: 4000000 INFO @ Sat, 15 Jan 2022 19:55:58: 8000000 INFO @ Sat, 15 Jan 2022 19:56:02: 5000000 INFO @ Sat, 15 Jan 2022 19:56:04: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:56:09: 6000000 INFO @ Sat, 15 Jan 2022 19:56:11: 10000000 INFO @ Sat, 15 Jan 2022 19:56:16: 7000000 INFO @ Sat, 15 Jan 2022 19:56:18: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:56:22: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:56:22: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:56:22: #1 total tags in treatment: 11650137 INFO @ Sat, 15 Jan 2022 19:56:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:56:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:56:23: #1 tags after filtering in treatment: 11650137 INFO @ Sat, 15 Jan 2022 19:56:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:56:23: #1 finished! INFO @ Sat, 15 Jan 2022 19:56:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:56:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:56:23: 8000000 INFO @ Sat, 15 Jan 2022 19:56:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:56:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:56:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:56:29: 9000000 INFO @ Sat, 15 Jan 2022 19:56:37: 10000000 INFO @ Sat, 15 Jan 2022 19:56:44: 11000000 INFO @ Sat, 15 Jan 2022 19:56:48: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:56:48: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:56:48: #1 total tags in treatment: 11650137 INFO @ Sat, 15 Jan 2022 19:56:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:56:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:56:48: #1 tags after filtering in treatment: 11650137 INFO @ Sat, 15 Jan 2022 19:56:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:56:48: #1 finished! INFO @ Sat, 15 Jan 2022 19:56:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:56:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:56:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:56:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:56:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119840/SRX7119840.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling