Job ID = 14520694 SRX = SRX7119837 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25883652 spots for SRR10423746/SRR10423746.sra Written 25883652 spots for SRR10423746/SRR10423746.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:49 25883652 reads; of these: 25883652 (100.00%) were unpaired; of these: 1499178 (5.79%) aligned 0 times 21339522 (82.44%) aligned exactly 1 time 3044952 (11.76%) aligned >1 times 94.21% overall alignment rate Time searching: 00:05:49 Overall time: 00:05:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11115983 / 24384474 = 0.4559 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:51:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:51:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:51:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:52:04: 1000000 INFO @ Sat, 15 Jan 2022 19:52:12: 2000000 INFO @ Sat, 15 Jan 2022 19:52:19: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:52:26: 4000000 INFO @ Sat, 15 Jan 2022 19:52:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:52:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:52:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:52:33: 5000000 INFO @ Sat, 15 Jan 2022 19:52:34: 1000000 INFO @ Sat, 15 Jan 2022 19:52:40: 6000000 INFO @ Sat, 15 Jan 2022 19:52:41: 2000000 INFO @ Sat, 15 Jan 2022 19:52:47: 7000000 INFO @ Sat, 15 Jan 2022 19:52:48: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:52:54: 8000000 INFO @ Sat, 15 Jan 2022 19:52:55: 4000000 INFO @ Sat, 15 Jan 2022 19:52:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:52:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:52:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:53:01: 9000000 INFO @ Sat, 15 Jan 2022 19:53:02: 5000000 INFO @ Sat, 15 Jan 2022 19:53:04: 1000000 INFO @ Sat, 15 Jan 2022 19:53:07: 10000000 INFO @ Sat, 15 Jan 2022 19:53:10: 6000000 INFO @ Sat, 15 Jan 2022 19:53:11: 2000000 INFO @ Sat, 15 Jan 2022 19:53:14: 11000000 INFO @ Sat, 15 Jan 2022 19:53:17: 7000000 INFO @ Sat, 15 Jan 2022 19:53:19: 3000000 INFO @ Sat, 15 Jan 2022 19:53:20: 12000000 INFO @ Sat, 15 Jan 2022 19:53:24: 8000000 INFO @ Sat, 15 Jan 2022 19:53:26: 4000000 INFO @ Sat, 15 Jan 2022 19:53:27: 13000000 INFO @ Sat, 15 Jan 2022 19:53:29: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:53:29: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:53:29: #1 total tags in treatment: 13268491 INFO @ Sat, 15 Jan 2022 19:53:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:53:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:53:30: #1 tags after filtering in treatment: 13268491 INFO @ Sat, 15 Jan 2022 19:53:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:53:30: #1 finished! INFO @ Sat, 15 Jan 2022 19:53:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:53:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:53:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:53:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:53:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 19:53:31: 9000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:53:33: 5000000 INFO @ Sat, 15 Jan 2022 19:53:38: 10000000 INFO @ Sat, 15 Jan 2022 19:53:40: 6000000 INFO @ Sat, 15 Jan 2022 19:53:45: 11000000 INFO @ Sat, 15 Jan 2022 19:53:47: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:53:52: 12000000 INFO @ Sat, 15 Jan 2022 19:53:54: 8000000 INFO @ Sat, 15 Jan 2022 19:53:59: 13000000 INFO @ Sat, 15 Jan 2022 19:54:01: 9000000 INFO @ Sat, 15 Jan 2022 19:54:01: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:54:01: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:54:01: #1 total tags in treatment: 13268491 INFO @ Sat, 15 Jan 2022 19:54:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:01: #1 tags after filtering in treatment: 13268491 INFO @ Sat, 15 Jan 2022 19:54:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:54:01: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:54:07: 10000000 INFO @ Sat, 15 Jan 2022 19:54:14: 11000000 INFO @ Sat, 15 Jan 2022 19:54:21: 12000000 INFO @ Sat, 15 Jan 2022 19:54:28: 13000000 INFO @ Sat, 15 Jan 2022 19:54:30: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:54:30: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:54:30: #1 total tags in treatment: 13268491 INFO @ Sat, 15 Jan 2022 19:54:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:30: #1 tags after filtering in treatment: 13268491 INFO @ Sat, 15 Jan 2022 19:54:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:54:30: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119837/SRX7119837.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling