Job ID = 14520693 SRX = SRX7119836 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 31040985 spots for SRR10423745/SRR10423745.sra Written 31040985 spots for SRR10423745/SRR10423745.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:10 31040985 reads; of these: 31040985 (100.00%) were unpaired; of these: 1273741 (4.10%) aligned 0 times 28149250 (90.68%) aligned exactly 1 time 1617994 (5.21%) aligned >1 times 95.90% overall alignment rate Time searching: 00:04:10 Overall time: 00:04:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 17579949 / 29767244 = 0.5906 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:28: 1000000 INFO @ Sat, 15 Jan 2022 19:49:33: 2000000 INFO @ Sat, 15 Jan 2022 19:49:38: 3000000 INFO @ Sat, 15 Jan 2022 19:49:43: 4000000 INFO @ Sat, 15 Jan 2022 19:49:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:52: 6000000 INFO @ Sat, 15 Jan 2022 19:49:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:57: 7000000 INFO @ Sat, 15 Jan 2022 19:50:00: 1000000 INFO @ Sat, 15 Jan 2022 19:50:03: 8000000 INFO @ Sat, 15 Jan 2022 19:50:06: 2000000 INFO @ Sat, 15 Jan 2022 19:50:08: 9000000 INFO @ Sat, 15 Jan 2022 19:50:12: 3000000 INFO @ Sat, 15 Jan 2022 19:50:14: 10000000 INFO @ Sat, 15 Jan 2022 19:50:19: 4000000 INFO @ Sat, 15 Jan 2022 19:50:19: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:24: 12000000 INFO @ Sat, 15 Jan 2022 19:50:25: 5000000 INFO @ Sat, 15 Jan 2022 19:50:25: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:50:25: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:50:25: #1 total tags in treatment: 12187295 INFO @ Sat, 15 Jan 2022 19:50:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:26: #1 tags after filtering in treatment: 12187295 INFO @ Sat, 15 Jan 2022 19:50:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:50:26: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:50:29: 1000000 INFO @ Sat, 15 Jan 2022 19:50:31: 6000000 INFO @ Sat, 15 Jan 2022 19:50:34: 2000000 INFO @ Sat, 15 Jan 2022 19:50:37: 7000000 INFO @ Sat, 15 Jan 2022 19:50:39: 3000000 INFO @ Sat, 15 Jan 2022 19:50:43: 8000000 INFO @ Sat, 15 Jan 2022 19:50:45: 4000000 INFO @ Sat, 15 Jan 2022 19:50:49: 9000000 INFO @ Sat, 15 Jan 2022 19:50:50: 5000000 INFO @ Sat, 15 Jan 2022 19:50:55: 10000000 INFO @ Sat, 15 Jan 2022 19:50:56: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:51:01: 7000000 INFO @ Sat, 15 Jan 2022 19:51:01: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:51:07: 8000000 INFO @ Sat, 15 Jan 2022 19:51:08: 12000000 INFO @ Sat, 15 Jan 2022 19:51:09: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:51:09: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:51:09: #1 total tags in treatment: 12187295 INFO @ Sat, 15 Jan 2022 19:51:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:09: #1 tags after filtering in treatment: 12187295 INFO @ Sat, 15 Jan 2022 19:51:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:09: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:51:12: 9000000 INFO @ Sat, 15 Jan 2022 19:51:17: 10000000 INFO @ Sat, 15 Jan 2022 19:51:21: 11000000 INFO @ Sat, 15 Jan 2022 19:51:26: 12000000 INFO @ Sat, 15 Jan 2022 19:51:27: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:51:27: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:51:27: #1 total tags in treatment: 12187295 INFO @ Sat, 15 Jan 2022 19:51:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:27: #1 tags after filtering in treatment: 12187295 INFO @ Sat, 15 Jan 2022 19:51:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:27: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7119836/SRX7119836.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling