Job ID = 4289604 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,225,126 reads read : 50,450,252 reads written : 25,225,126 reads 0-length : 25,225,126 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:26 25225126 reads; of these: 25225126 (100.00%) were unpaired; of these: 1431869 (5.68%) aligned 0 times 19277120 (76.42%) aligned exactly 1 time 4516137 (17.90%) aligned >1 times 94.32% overall alignment rate Time searching: 00:04:27 Overall time: 00:04:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12239730 / 23793257 = 0.5144 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 15:08:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 15:08:27: #1 read tag files... INFO @ Tue, 10 Dec 2019 15:08:27: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 15:08:35: 1000000 INFO @ Tue, 10 Dec 2019 15:08:42: 2000000 INFO @ Tue, 10 Dec 2019 15:08:49: 3000000 INFO @ Tue, 10 Dec 2019 15:08:56: 4000000 INFO @ Tue, 10 Dec 2019 15:08:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 15:08:57: #1 read tag files... INFO @ Tue, 10 Dec 2019 15:08:57: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 15:09:04: 5000000 INFO @ Tue, 10 Dec 2019 15:09:06: 1000000 INFO @ Tue, 10 Dec 2019 15:09:13: 6000000 INFO @ Tue, 10 Dec 2019 15:09:15: 2000000 INFO @ Tue, 10 Dec 2019 15:09:22: 7000000 INFO @ Tue, 10 Dec 2019 15:09:24: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 15:09:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 15:09:27: #1 read tag files... INFO @ Tue, 10 Dec 2019 15:09:27: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 15:09:31: 8000000 INFO @ Tue, 10 Dec 2019 15:09:33: 4000000 INFO @ Tue, 10 Dec 2019 15:09:35: 1000000 INFO @ Tue, 10 Dec 2019 15:09:40: 9000000 INFO @ Tue, 10 Dec 2019 15:09:42: 5000000 INFO @ Tue, 10 Dec 2019 15:09:43: 2000000 INFO @ Tue, 10 Dec 2019 15:09:50: 10000000 INFO @ Tue, 10 Dec 2019 15:09:51: 3000000 INFO @ Tue, 10 Dec 2019 15:09:51: 6000000 INFO @ Tue, 10 Dec 2019 15:09:59: 4000000 INFO @ Tue, 10 Dec 2019 15:09:59: 11000000 INFO @ Tue, 10 Dec 2019 15:10:00: 7000000 INFO @ Tue, 10 Dec 2019 15:10:03: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 15:10:03: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 15:10:03: #1 total tags in treatment: 11553527 INFO @ Tue, 10 Dec 2019 15:10:03: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 15:10:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 15:10:04: #1 tags after filtering in treatment: 11553527 INFO @ Tue, 10 Dec 2019 15:10:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 15:10:04: #1 finished! INFO @ Tue, 10 Dec 2019 15:10:04: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 15:10:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 15:10:05: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 15:10:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 15:10:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 15:10:06: 5000000 INFO @ Tue, 10 Dec 2019 15:10:08: 8000000 INFO @ Tue, 10 Dec 2019 15:10:14: 6000000 INFO @ Tue, 10 Dec 2019 15:10:16: 9000000 INFO @ Tue, 10 Dec 2019 15:10:21: 7000000 INFO @ Tue, 10 Dec 2019 15:10:23: 10000000 INFO @ Tue, 10 Dec 2019 15:10:28: 8000000 INFO @ Tue, 10 Dec 2019 15:10:31: 11000000 INFO @ Tue, 10 Dec 2019 15:10:35: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 15:10:35: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 15:10:35: #1 total tags in treatment: 11553527 INFO @ Tue, 10 Dec 2019 15:10:35: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 15:10:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 15:10:35: #1 tags after filtering in treatment: 11553527 INFO @ Tue, 10 Dec 2019 15:10:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 15:10:35: #1 finished! INFO @ Tue, 10 Dec 2019 15:10:35: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 15:10:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 15:10:36: 9000000 INFO @ Tue, 10 Dec 2019 15:10:36: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 15:10:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 15:10:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 15:10:44: 10000000 INFO @ Tue, 10 Dec 2019 15:10:51: 11000000 INFO @ Tue, 10 Dec 2019 15:10:55: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 15:10:55: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 15:10:55: #1 total tags in treatment: 11553527 INFO @ Tue, 10 Dec 2019 15:10:55: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 15:10:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 15:10:56: #1 tags after filtering in treatment: 11553527 INFO @ Tue, 10 Dec 2019 15:10:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 15:10:56: #1 finished! INFO @ Tue, 10 Dec 2019 15:10:56: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 15:10:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 15:10:56: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 15:10:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 15:10:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106975/SRX7106975.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。