Job ID = 4289599 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,478,579 reads read : 34,957,158 reads written : 17,478,579 reads 0-length : 17,478,579 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:18 17478579 reads; of these: 17478579 (100.00%) were unpaired; of these: 792470 (4.53%) aligned 0 times 12557098 (71.84%) aligned exactly 1 time 4129011 (23.62%) aligned >1 times 95.47% overall alignment rate Time searching: 00:03:18 Overall time: 00:03:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10442014 / 16686109 = 0.6258 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 15:02:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 15:02:52: #1 read tag files... INFO @ Tue, 10 Dec 2019 15:02:52: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 15:02:59: 1000000 INFO @ Tue, 10 Dec 2019 15:03:06: 2000000 INFO @ Tue, 10 Dec 2019 15:03:13: 3000000 INFO @ Tue, 10 Dec 2019 15:03:20: 4000000 INFO @ Tue, 10 Dec 2019 15:03:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 15:03:22: #1 read tag files... INFO @ Tue, 10 Dec 2019 15:03:22: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 15:03:27: 5000000 INFO @ Tue, 10 Dec 2019 15:03:29: 1000000 INFO @ Tue, 10 Dec 2019 15:03:34: 6000000 INFO @ Tue, 10 Dec 2019 15:03:35: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 15:03:35: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 15:03:35: #1 total tags in treatment: 6244095 INFO @ Tue, 10 Dec 2019 15:03:35: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 15:03:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 15:03:35: #1 tags after filtering in treatment: 6244095 INFO @ Tue, 10 Dec 2019 15:03:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 15:03:35: #1 finished! INFO @ Tue, 10 Dec 2019 15:03:35: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 15:03:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 15:03:36: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 15:03:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 15:03:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 15:03:37: 2000000 INFO @ Tue, 10 Dec 2019 15:03:44: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 15:03:50: 4000000 INFO @ Tue, 10 Dec 2019 15:03:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 15:03:52: #1 read tag files... INFO @ Tue, 10 Dec 2019 15:03:52: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 15:03:57: 5000000 INFO @ Tue, 10 Dec 2019 15:04:01: 1000000 INFO @ Tue, 10 Dec 2019 15:04:04: 6000000 INFO @ Tue, 10 Dec 2019 15:04:06: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 15:04:06: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 15:04:06: #1 total tags in treatment: 6244095 INFO @ Tue, 10 Dec 2019 15:04:06: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 15:04:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 15:04:06: #1 tags after filtering in treatment: 6244095 INFO @ Tue, 10 Dec 2019 15:04:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 15:04:06: #1 finished! INFO @ Tue, 10 Dec 2019 15:04:06: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 15:04:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 15:04:07: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 15:04:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 15:04:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 15:04:10: 2000000 INFO @ Tue, 10 Dec 2019 15:04:18: 3000000 INFO @ Tue, 10 Dec 2019 15:04:25: 4000000 INFO @ Tue, 10 Dec 2019 15:04:33: 5000000 INFO @ Tue, 10 Dec 2019 15:04:41: 6000000 INFO @ Tue, 10 Dec 2019 15:04:43: #1 tag size is determined as 50 bps INFO @ Tue, 10 Dec 2019 15:04:43: #1 tag size = 50 INFO @ Tue, 10 Dec 2019 15:04:43: #1 total tags in treatment: 6244095 INFO @ Tue, 10 Dec 2019 15:04:43: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 15:04:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 15:04:43: #1 tags after filtering in treatment: 6244095 INFO @ Tue, 10 Dec 2019 15:04:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 15:04:43: #1 finished! INFO @ Tue, 10 Dec 2019 15:04:43: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 15:04:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 15:04:44: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 15:04:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 15:04:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7106971/SRX7106971.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。