Job ID = 9163284 sra ファイルのダウンロード中... Completed: 9657692K bytes transferred in 75 seconds (1045343K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 154547274 spots for /home/okishinya/chipatlas/results/sacCer3/SRX707476/SRR1582479.sra Written 154547274 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:37:07 154547274 reads; of these: 154547274 (100.00%) were unpaired; of these: 21637845 (14.00%) aligned 0 times 53272525 (34.47%) aligned exactly 1 time 79636904 (51.53%) aligned >1 times 86.00% overall alignment rate Time searching: 00:37:07 Overall time: 00:37:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 56 files... [bam_rmdupse_core] 116296206 / 132909429 = 0.8750 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:44:24: # Command line: callpeak -t SRX707476.bam -f BAM -g 12100000 -n SRX707476.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX707476.05 # format = BAM # ChIP-seq file = ['SRX707476.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:44:24: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:44:24: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:44:24: # Command line: callpeak -t SRX707476.bam -f BAM -g 12100000 -n SRX707476.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX707476.10 # format = BAM # ChIP-seq file = ['SRX707476.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:44:24: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:44:24: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:44:24: # Command line: callpeak -t SRX707476.bam -f BAM -g 12100000 -n SRX707476.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX707476.20 # format = BAM # ChIP-seq file = ['SRX707476.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:44:24: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:44:24: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:44:33: 1000000 INFO @ Wed, 28 Jun 2017 11:44:33: 1000000 INFO @ Wed, 28 Jun 2017 11:44:33: 1000000 INFO @ Wed, 28 Jun 2017 11:44:42: 2000000 INFO @ Wed, 28 Jun 2017 11:44:43: 2000000 INFO @ Wed, 28 Jun 2017 11:44:43: 2000000 INFO @ Wed, 28 Jun 2017 11:44:51: 3000000 INFO @ Wed, 28 Jun 2017 11:44:52: 3000000 INFO @ Wed, 28 Jun 2017 11:44:52: 3000000 INFO @ Wed, 28 Jun 2017 11:45:00: 4000000 INFO @ Wed, 28 Jun 2017 11:45:01: 4000000 INFO @ Wed, 28 Jun 2017 11:45:01: 4000000 INFO @ Wed, 28 Jun 2017 11:45:09: 5000000 INFO @ Wed, 28 Jun 2017 11:45:10: 5000000 INFO @ Wed, 28 Jun 2017 11:45:10: 5000000 INFO @ Wed, 28 Jun 2017 11:45:18: 6000000 INFO @ Wed, 28 Jun 2017 11:45:19: 6000000 INFO @ Wed, 28 Jun 2017 11:45:19: 6000000 INFO @ Wed, 28 Jun 2017 11:45:27: 7000000 INFO @ Wed, 28 Jun 2017 11:45:28: 7000000 INFO @ Wed, 28 Jun 2017 11:45:28: 7000000 INFO @ Wed, 28 Jun 2017 11:45:36: 8000000 INFO @ Wed, 28 Jun 2017 11:45:37: 8000000 INFO @ Wed, 28 Jun 2017 11:45:38: 8000000 INFO @ Wed, 28 Jun 2017 11:45:46: 9000000 INFO @ Wed, 28 Jun 2017 11:45:46: 9000000 INFO @ Wed, 28 Jun 2017 11:45:48: 9000000 INFO @ Wed, 28 Jun 2017 11:45:55: 10000000 INFO @ Wed, 28 Jun 2017 11:45:57: 10000000 INFO @ Wed, 28 Jun 2017 11:45:58: 10000000 INFO @ Wed, 28 Jun 2017 11:46:04: 11000000 INFO @ Wed, 28 Jun 2017 11:46:07: 11000000 INFO @ Wed, 28 Jun 2017 11:46:09: 11000000 INFO @ Wed, 28 Jun 2017 11:46:12: 12000000 INFO @ Wed, 28 Jun 2017 11:46:17: 12000000 INFO @ Wed, 28 Jun 2017 11:46:19: 12000000 INFO @ Wed, 28 Jun 2017 11:46:21: 13000000 INFO @ Wed, 28 Jun 2017 11:46:29: 13000000 INFO @ Wed, 28 Jun 2017 11:46:30: 14000000 INFO @ Wed, 28 Jun 2017 11:46:30: 13000000 INFO @ Wed, 28 Jun 2017 11:46:38: 15000000 INFO @ Wed, 28 Jun 2017 11:46:39: 14000000 INFO @ Wed, 28 Jun 2017 11:46:41: 14000000 INFO @ Wed, 28 Jun 2017 11:46:47: 16000000 INFO @ Wed, 28 Jun 2017 11:46:50: 15000000 INFO @ Wed, 28 Jun 2017 11:46:52: 15000000 INFO @ Wed, 28 Jun 2017 11:46:53: #1 tag size is determined as 101 bps INFO @ Wed, 28 Jun 2017 11:46:53: #1 tag size = 101 INFO @ Wed, 28 Jun 2017 11:46:53: #1 total tags in treatment: 16613223 INFO @ Wed, 28 Jun 2017 11:46:53: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:46:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:46:53: #1 tags after filtering in treatment: 16613223 INFO @ Wed, 28 Jun 2017 11:46:53: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:46:53: #1 finished! INFO @ Wed, 28 Jun 2017 11:46:53: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:46:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:46:54: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:46:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:46:54: Process for pairing-model is terminated! cat: SRX707476.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX707476.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707476.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707476.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:47:00: 16000000 INFO @ Wed, 28 Jun 2017 11:47:02: 16000000 INFO @ Wed, 28 Jun 2017 11:47:06: #1 tag size is determined as 101 bps INFO @ Wed, 28 Jun 2017 11:47:06: #1 tag size = 101 INFO @ Wed, 28 Jun 2017 11:47:06: #1 total tags in treatment: 16613223 INFO @ Wed, 28 Jun 2017 11:47:06: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:47:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:47:06: #1 tags after filtering in treatment: 16613223 INFO @ Wed, 28 Jun 2017 11:47:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:47:06: #1 finished! INFO @ Wed, 28 Jun 2017 11:47:06: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:47:06: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 28 Jun 2017 11:47:08: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:47:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:47:08: Process for pairing-model is terminated! cat: SRX707476.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX707476.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707476.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707476.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:47:08: #1 tag size is determined as 101 bps INFO @ Wed, 28 Jun 2017 11:47:08: #1 tag size = 101 INFO @ Wed, 28 Jun 2017 11:47:08: #1 total tags in treatment: 16613223 INFO @ Wed, 28 Jun 2017 11:47:08: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:47:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:47:08: #1 tags after filtering in treatment: 16613223 INFO @ Wed, 28 Jun 2017 11:47:08: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:47:08: #1 finished! INFO @ Wed, 28 Jun 2017 11:47:08: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:47:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:47:09: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:47:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:47:09: Process for pairing-model is terminated! cat: SRX707476.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX707476.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707476.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707476.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BigWig に変換しました。