Job ID = 9163281 sra ファイルのダウンロード中... Completed: 7625534K bytes transferred in 67 seconds (923016K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 123596105 spots for /home/okishinya/chipatlas/results/sacCer3/SRX707473/SRR1582476.sra Written 123596105 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:34 123596105 reads; of these: 123596105 (100.00%) were unpaired; of these: 86634227 (70.09%) aligned 0 times 25169134 (20.36%) aligned exactly 1 time 11792744 (9.54%) aligned >1 times 29.91% overall alignment rate Time searching: 00:22:34 Overall time: 00:22:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 25921662 / 36961878 = 0.7013 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:06:31: # Command line: callpeak -t SRX707473.bam -f BAM -g 12100000 -n SRX707473.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX707473.10 # format = BAM # ChIP-seq file = ['SRX707473.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:06:31: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:06:31: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:06:31: # Command line: callpeak -t SRX707473.bam -f BAM -g 12100000 -n SRX707473.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX707473.05 # format = BAM # ChIP-seq file = ['SRX707473.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:06:31: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:06:31: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:06:31: # Command line: callpeak -t SRX707473.bam -f BAM -g 12100000 -n SRX707473.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX707473.20 # format = BAM # ChIP-seq file = ['SRX707473.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:06:31: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:06:31: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:06:39: 1000000 INFO @ Wed, 28 Jun 2017 11:06:39: 1000000 INFO @ Wed, 28 Jun 2017 11:06:40: 1000000 INFO @ Wed, 28 Jun 2017 11:06:48: 2000000 INFO @ Wed, 28 Jun 2017 11:06:48: 2000000 INFO @ Wed, 28 Jun 2017 11:06:48: 2000000 INFO @ Wed, 28 Jun 2017 11:06:57: 3000000 INFO @ Wed, 28 Jun 2017 11:06:57: 3000000 INFO @ Wed, 28 Jun 2017 11:06:57: 3000000 INFO @ Wed, 28 Jun 2017 11:07:06: 4000000 INFO @ Wed, 28 Jun 2017 11:07:06: 4000000 INFO @ Wed, 28 Jun 2017 11:07:06: 4000000 INFO @ Wed, 28 Jun 2017 11:07:15: 5000000 INFO @ Wed, 28 Jun 2017 11:07:16: 5000000 INFO @ Wed, 28 Jun 2017 11:07:16: 5000000 INFO @ Wed, 28 Jun 2017 11:07:23: 6000000 INFO @ Wed, 28 Jun 2017 11:07:25: 6000000 INFO @ Wed, 28 Jun 2017 11:07:25: 6000000 INFO @ Wed, 28 Jun 2017 11:07:32: 7000000 INFO @ Wed, 28 Jun 2017 11:07:34: 7000000 INFO @ Wed, 28 Jun 2017 11:07:34: 7000000 INFO @ Wed, 28 Jun 2017 11:07:40: 8000000 INFO @ Wed, 28 Jun 2017 11:07:43: 8000000 INFO @ Wed, 28 Jun 2017 11:07:43: 8000000 INFO @ Wed, 28 Jun 2017 11:07:49: 9000000 INFO @ Wed, 28 Jun 2017 11:07:51: 9000000 INFO @ Wed, 28 Jun 2017 11:07:51: 9000000 INFO @ Wed, 28 Jun 2017 11:07:57: 10000000 INFO @ Wed, 28 Jun 2017 11:07:59: 10000000 INFO @ Wed, 28 Jun 2017 11:07:59: 10000000 INFO @ Wed, 28 Jun 2017 11:08:05: 11000000 INFO @ Wed, 28 Jun 2017 11:08:05: #1 tag size is determined as 101 bps INFO @ Wed, 28 Jun 2017 11:08:05: #1 tag size = 101 INFO @ Wed, 28 Jun 2017 11:08:05: #1 total tags in treatment: 11040216 INFO @ Wed, 28 Jun 2017 11:08:05: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:06: #1 tags after filtering in treatment: 11040216 INFO @ Wed, 28 Jun 2017 11:08:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:06: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:06: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:06: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:06: Process for pairing-model is terminated! cat: SRX707473.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX707473.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707473.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707473.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:08:08: 11000000 INFO @ Wed, 28 Jun 2017 11:08:08: 11000000 INFO @ Wed, 28 Jun 2017 11:08:08: #1 tag size is determined as 101 bps INFO @ Wed, 28 Jun 2017 11:08:08: #1 tag size = 101 INFO @ Wed, 28 Jun 2017 11:08:08: #1 total tags in treatment: 11040216 INFO @ Wed, 28 Jun 2017 11:08:08: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:08: #1 tag size is determined as 101 bps INFO @ Wed, 28 Jun 2017 11:08:08: #1 tag size = 101 INFO @ Wed, 28 Jun 2017 11:08:08: #1 total tags in treatment: 11040216 INFO @ Wed, 28 Jun 2017 11:08:08: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:08: #1 tags after filtering in treatment: 11040216 INFO @ Wed, 28 Jun 2017 11:08:08: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:08: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:08: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:08: #1 tags after filtering in treatment: 11040216 INFO @ Wed, 28 Jun 2017 11:08:08: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:08: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:08: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:09: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. INFO @ Wed, 28 Jun 2017 11:08:09: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:09: Process for pairing-model is terminated! WARNING @ Wed, 28 Jun 2017 11:08:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:09: Process for pairing-model is terminated! cat: SRX707473.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX707473.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX707473.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707473.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707473.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `SRX707473.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707473.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707473.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。