Job ID = 9163280 sra ファイルのダウンロード中... Completed: 1361920K bytes transferred in 15 seconds (708203K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20477493 spots for /home/okishinya/chipatlas/results/sacCer3/SRX707472/SRR1582475.sra Written 20477493 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:33 20477493 reads; of these: 20477493 (100.00%) were unpaired; of these: 4839134 (23.63%) aligned 0 times 11207531 (54.73%) aligned exactly 1 time 4430828 (21.64%) aligned >1 times 76.37% overall alignment rate Time searching: 00:10:33 Overall time: 00:10:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10341504 / 15638359 = 0.6613 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 10:40:52: # Command line: callpeak -t SRX707472.bam -f BAM -g 12100000 -n SRX707472.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX707472.20 # format = BAM # ChIP-seq file = ['SRX707472.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:40:52: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:40:52: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:40:52: # Command line: callpeak -t SRX707472.bam -f BAM -g 12100000 -n SRX707472.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX707472.05 # format = BAM # ChIP-seq file = ['SRX707472.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:40:52: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:40:52: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:40:52: # Command line: callpeak -t SRX707472.bam -f BAM -g 12100000 -n SRX707472.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX707472.10 # format = BAM # ChIP-seq file = ['SRX707472.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:40:52: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:40:52: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:41:00: 1000000 INFO @ Wed, 28 Jun 2017 10:41:00: 1000000 INFO @ Wed, 28 Jun 2017 10:41:00: 1000000 INFO @ Wed, 28 Jun 2017 10:41:08: 2000000 INFO @ Wed, 28 Jun 2017 10:41:08: 2000000 INFO @ Wed, 28 Jun 2017 10:41:08: 2000000 INFO @ Wed, 28 Jun 2017 10:41:17: 3000000 INFO @ Wed, 28 Jun 2017 10:41:17: 3000000 INFO @ Wed, 28 Jun 2017 10:41:17: 3000000 INFO @ Wed, 28 Jun 2017 10:41:27: 4000000 INFO @ Wed, 28 Jun 2017 10:41:28: 4000000 INFO @ Wed, 28 Jun 2017 10:41:28: 4000000 INFO @ Wed, 28 Jun 2017 10:41:38: 5000000 INFO @ Wed, 28 Jun 2017 10:41:38: 5000000 INFO @ Wed, 28 Jun 2017 10:41:38: 5000000 INFO @ Wed, 28 Jun 2017 10:41:40: #1 tag size is determined as 100 bps INFO @ Wed, 28 Jun 2017 10:41:40: #1 tag size = 100 INFO @ Wed, 28 Jun 2017 10:41:40: #1 total tags in treatment: 5296855 INFO @ Wed, 28 Jun 2017 10:41:40: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:41:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:41:40: #1 tags after filtering in treatment: 5296855 INFO @ Wed, 28 Jun 2017 10:41:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:41:40: #1 finished! INFO @ Wed, 28 Jun 2017 10:41:40: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:41:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:41:41: #1 tag size is determined as 100 bps INFO @ Wed, 28 Jun 2017 10:41:41: #1 tag size = 100 INFO @ Wed, 28 Jun 2017 10:41:41: #1 total tags in treatment: 5296855 INFO @ Wed, 28 Jun 2017 10:41:41: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:41:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:41:41: #1 tags after filtering in treatment: 5296855 INFO @ Wed, 28 Jun 2017 10:41:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:41:41: #1 finished! INFO @ Wed, 28 Jun 2017 10:41:41: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:41:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:41:41: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:41:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:41:41: Process for pairing-model is terminated! cat: SRX707472.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX707472.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707472.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707472.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 10:41:41: #1 tag size is determined as 100 bps INFO @ Wed, 28 Jun 2017 10:41:41: #1 tag size = 100 INFO @ Wed, 28 Jun 2017 10:41:41: #1 total tags in treatment: 5296855 INFO @ Wed, 28 Jun 2017 10:41:41: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:41:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:41:41: #1 tags after filtering in treatment: 5296855 INFO @ Wed, 28 Jun 2017 10:41:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:41:41: #1 finished! INFO @ Wed, 28 Jun 2017 10:41:41: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:41:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:41:41: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:41:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:41:41: Process for pairing-model is terminated! cat: SRX707472.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX707472.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707472.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707472.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 10:41:41: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:41:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:41:41: Process for pairing-model is terminated! cat: SRX707472.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX707472.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707472.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX707472.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。