Job ID = 7107566 SRX = SRX696010 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15218733 spots for SRR1569575/SRR1569575.sra Written 15218733 spots for SRR1569575/SRR1569575.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:43 15218733 reads; of these: 15218733 (100.00%) were unpaired; of these: 851091 (5.59%) aligned 0 times 12159037 (79.90%) aligned exactly 1 time 2208605 (14.51%) aligned >1 times 94.41% overall alignment rate Time searching: 00:01:43 Overall time: 00:01:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4452935 / 14367642 = 0.3099 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:13:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:13:52: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:13:52: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:13:56: 1000000 INFO @ Wed, 22 Jul 2020 13:14:00: 2000000 INFO @ Wed, 22 Jul 2020 13:14:05: 3000000 INFO @ Wed, 22 Jul 2020 13:14:09: 4000000 INFO @ Wed, 22 Jul 2020 13:14:14: 5000000 INFO @ Wed, 22 Jul 2020 13:14:18: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:14:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:14:22: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:14:22: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:14:22: 7000000 INFO @ Wed, 22 Jul 2020 13:14:26: 1000000 INFO @ Wed, 22 Jul 2020 13:14:27: 8000000 INFO @ Wed, 22 Jul 2020 13:14:31: 2000000 INFO @ Wed, 22 Jul 2020 13:14:31: 9000000 INFO @ Wed, 22 Jul 2020 13:14:35: 3000000 INFO @ Wed, 22 Jul 2020 13:14:35: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 13:14:35: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 13:14:35: #1 total tags in treatment: 9914707 INFO @ Wed, 22 Jul 2020 13:14:35: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:14:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:14:36: #1 tags after filtering in treatment: 9914707 INFO @ Wed, 22 Jul 2020 13:14:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:14:36: #1 finished! INFO @ Wed, 22 Jul 2020 13:14:36: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:14:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:14:36: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:14:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:14:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:14:40: 4000000 INFO @ Wed, 22 Jul 2020 13:14:44: 5000000 INFO @ Wed, 22 Jul 2020 13:14:48: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:14:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:14:52: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:14:52: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:14:53: 7000000 INFO @ Wed, 22 Jul 2020 13:14:56: 1000000 INFO @ Wed, 22 Jul 2020 13:14:57: 8000000 INFO @ Wed, 22 Jul 2020 13:15:01: 2000000 INFO @ Wed, 22 Jul 2020 13:15:02: 9000000 INFO @ Wed, 22 Jul 2020 13:15:05: 3000000 INFO @ Wed, 22 Jul 2020 13:15:06: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 13:15:06: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 13:15:06: #1 total tags in treatment: 9914707 INFO @ Wed, 22 Jul 2020 13:15:06: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:15:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:15:06: #1 tags after filtering in treatment: 9914707 INFO @ Wed, 22 Jul 2020 13:15:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:15:06: #1 finished! INFO @ Wed, 22 Jul 2020 13:15:06: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:15:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:15:07: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:15:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:15:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:15:10: 4000000 INFO @ Wed, 22 Jul 2020 13:15:14: 5000000 INFO @ Wed, 22 Jul 2020 13:15:19: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:15:23: 7000000 INFO @ Wed, 22 Jul 2020 13:15:27: 8000000 INFO @ Wed, 22 Jul 2020 13:15:32: 9000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:15:36: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 13:15:36: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 13:15:36: #1 total tags in treatment: 9914707 INFO @ Wed, 22 Jul 2020 13:15:36: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:15:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:15:36: #1 tags after filtering in treatment: 9914707 INFO @ Wed, 22 Jul 2020 13:15:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:15:36: #1 finished! INFO @ Wed, 22 Jul 2020 13:15:36: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:15:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:15:37: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:15:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:15:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX696010/SRX696010.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling