Job ID = 14521990 SRX = SRX6950516 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 2545252 spots for SRR10231164/SRR10231164.sra Written 2545252 spots for SRR10231164/SRR10231164.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:19 2545252 reads; of these: 2545252 (100.00%) were paired; of these: 679052 (26.68%) aligned concordantly 0 times 1683673 (66.15%) aligned concordantly exactly 1 time 182527 (7.17%) aligned concordantly >1 times ---- 679052 pairs aligned concordantly 0 times; of these: 269276 (39.65%) aligned discordantly 1 time ---- 409776 pairs aligned 0 times concordantly or discordantly; of these: 819552 mates make up the pairs; of these: 655676 (80.00%) aligned 0 times 105330 (12.85%) aligned exactly 1 time 58546 (7.14%) aligned >1 times 87.12% overall alignment rate Time searching: 00:04:19 Overall time: 00:04:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 478822 / 1117448 = 0.4285 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:03:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:03:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:03:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:03:41: 1000000 INFO @ Sat, 15 Jan 2022 22:03:53: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:04:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:04:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:04:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:04:04: 3000000 INFO @ Sat, 15 Jan 2022 22:04:10: #1 tag size is determined as 96 bps INFO @ Sat, 15 Jan 2022 22:04:10: #1 tag size = 96 INFO @ Sat, 15 Jan 2022 22:04:10: #1 total tags in treatment: 1491019 INFO @ Sat, 15 Jan 2022 22:04:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:04:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:04:10: #1 tags after filtering in treatment: 966739 INFO @ Sat, 15 Jan 2022 22:04:10: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 22:04:10: #1 finished! INFO @ Sat, 15 Jan 2022 22:04:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:04:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:04:10: #2 number of paired peaks: 49 WARNING @ Sat, 15 Jan 2022 22:04:10: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:04:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:04:13: 1000000 INFO @ Sat, 15 Jan 2022 22:04:25: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:04:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:04:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:04:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:04:37: 3000000 INFO @ Sat, 15 Jan 2022 22:04:42: 1000000 INFO @ Sat, 15 Jan 2022 22:04:42: #1 tag size is determined as 96 bps INFO @ Sat, 15 Jan 2022 22:04:42: #1 tag size = 96 INFO @ Sat, 15 Jan 2022 22:04:42: #1 total tags in treatment: 1491019 INFO @ Sat, 15 Jan 2022 22:04:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:04:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:04:42: #1 tags after filtering in treatment: 966739 INFO @ Sat, 15 Jan 2022 22:04:42: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 22:04:42: #1 finished! INFO @ Sat, 15 Jan 2022 22:04:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:04:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:04:42: #2 number of paired peaks: 49 WARNING @ Sat, 15 Jan 2022 22:04:42: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:04:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:04:55: 2000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:05:07: 3000000 INFO @ Sat, 15 Jan 2022 22:05:13: #1 tag size is determined as 96 bps INFO @ Sat, 15 Jan 2022 22:05:13: #1 tag size = 96 INFO @ Sat, 15 Jan 2022 22:05:13: #1 total tags in treatment: 1491019 INFO @ Sat, 15 Jan 2022 22:05:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:05:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:05:13: #1 tags after filtering in treatment: 966739 INFO @ Sat, 15 Jan 2022 22:05:13: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 22:05:13: #1 finished! INFO @ Sat, 15 Jan 2022 22:05:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:05:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:05:13: #2 number of paired peaks: 49 WARNING @ Sat, 15 Jan 2022 22:05:13: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:05:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950516/SRX6950516.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling