Job ID = 14521964 SRX = SRX6950508 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3657419 spots for SRR10231168/SRR10231168.sra Written 3657419 spots for SRR10231168/SRR10231168.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:27 3657419 reads; of these: 3657419 (100.00%) were paired; of these: 738007 (20.18%) aligned concordantly 0 times 2650419 (72.47%) aligned concordantly exactly 1 time 268993 (7.35%) aligned concordantly >1 times ---- 738007 pairs aligned concordantly 0 times; of these: 360573 (48.86%) aligned discordantly 1 time ---- 377434 pairs aligned 0 times concordantly or discordantly; of these: 754868 mates make up the pairs; of these: 614444 (81.40%) aligned 0 times 58595 (7.76%) aligned exactly 1 time 81829 (10.84%) aligned >1 times 91.60% overall alignment rate Time searching: 00:04:27 Overall time: 00:04:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 298832 / 2653064 = 0.1126 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:01:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:01:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:01:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:05: 1000000 INFO @ Sat, 15 Jan 2022 22:02:13: 2000000 INFO @ Sat, 15 Jan 2022 22:02:21: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:29: 4000000 INFO @ Sat, 15 Jan 2022 22:02:34: 1000000 INFO @ Sat, 15 Jan 2022 22:02:37: 5000000 INFO @ Sat, 15 Jan 2022 22:02:42: 2000000 INFO @ Sat, 15 Jan 2022 22:02:45: 6000000 INFO @ Sat, 15 Jan 2022 22:02:46: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:02:46: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:02:46: #1 total tags in treatment: 2657172 INFO @ Sat, 15 Jan 2022 22:02:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:02:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:02:46: #1 tags after filtering in treatment: 2293304 INFO @ Sat, 15 Jan 2022 22:02:46: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 22:02:46: #1 finished! INFO @ Sat, 15 Jan 2022 22:02:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:02:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:02:46: #2 number of paired peaks: 61 WARNING @ Sat, 15 Jan 2022 22:02:46: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:02:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:02:49: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:56: 4000000 INFO @ Sat, 15 Jan 2022 22:02:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:03:03: 5000000 INFO @ Sat, 15 Jan 2022 22:03:06: 1000000 INFO @ Sat, 15 Jan 2022 22:03:10: 6000000 INFO @ Sat, 15 Jan 2022 22:03:11: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:03:11: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:03:11: #1 total tags in treatment: 2657172 INFO @ Sat, 15 Jan 2022 22:03:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:11: #1 tags after filtering in treatment: 2293304 INFO @ Sat, 15 Jan 2022 22:03:11: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 22:03:11: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:11: #2 number of paired peaks: 61 WARNING @ Sat, 15 Jan 2022 22:03:11: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:14: 2000000 INFO @ Sat, 15 Jan 2022 22:03:22: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:03:30: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:03:38: 5000000 INFO @ Sat, 15 Jan 2022 22:03:46: 6000000 INFO @ Sat, 15 Jan 2022 22:03:47: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:03:47: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:03:47: #1 total tags in treatment: 2657172 INFO @ Sat, 15 Jan 2022 22:03:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:47: #1 tags after filtering in treatment: 2293304 INFO @ Sat, 15 Jan 2022 22:03:47: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 22:03:47: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:47: #2 number of paired peaks: 61 WARNING @ Sat, 15 Jan 2022 22:03:47: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6950508/SRX6950508.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling