Job ID = 4289551 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,174,823 reads read : 6,174,823 reads written : 6,174,823 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:56 6174823 reads; of these: 6174823 (100.00%) were unpaired; of these: 481751 (7.80%) aligned 0 times 5388857 (87.27%) aligned exactly 1 time 304215 (4.93%) aligned >1 times 92.20% overall alignment rate Time searching: 00:00:56 Overall time: 00:00:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2533592 / 5693072 = 0.4450 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 14:50:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:50:20: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:50:20: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:50:27: 1000000 INFO @ Tue, 10 Dec 2019 14:50:34: 2000000 INFO @ Tue, 10 Dec 2019 14:50:41: 3000000 INFO @ Tue, 10 Dec 2019 14:50:42: #1 tag size is determined as 40 bps INFO @ Tue, 10 Dec 2019 14:50:42: #1 tag size = 40 INFO @ Tue, 10 Dec 2019 14:50:42: #1 total tags in treatment: 3159480 INFO @ Tue, 10 Dec 2019 14:50:42: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:50:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:50:42: #1 tags after filtering in treatment: 3159480 INFO @ Tue, 10 Dec 2019 14:50:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:50:42: #1 finished! INFO @ Tue, 10 Dec 2019 14:50:42: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:50:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:50:43: #2 number of paired peaks: 5 WARNING @ Tue, 10 Dec 2019 14:50:43: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:50:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 14:50:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:50:50: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:50:50: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:50:57: 1000000 INFO @ Tue, 10 Dec 2019 14:51:04: 2000000 INFO @ Tue, 10 Dec 2019 14:51:11: 3000000 INFO @ Tue, 10 Dec 2019 14:51:12: #1 tag size is determined as 40 bps INFO @ Tue, 10 Dec 2019 14:51:12: #1 tag size = 40 INFO @ Tue, 10 Dec 2019 14:51:12: #1 total tags in treatment: 3159480 INFO @ Tue, 10 Dec 2019 14:51:12: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:51:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:51:12: #1 tags after filtering in treatment: 3159480 INFO @ Tue, 10 Dec 2019 14:51:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:51:12: #1 finished! INFO @ Tue, 10 Dec 2019 14:51:12: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:51:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:51:13: #2 number of paired peaks: 5 WARNING @ Tue, 10 Dec 2019 14:51:13: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:51:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... INFO @ Tue, 10 Dec 2019 14:51:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 14:51:20: #1 read tag files... INFO @ Tue, 10 Dec 2019 14:51:20: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 14:51:28: 1000000 INFO @ Tue, 10 Dec 2019 14:51:35: 2000000 INFO @ Tue, 10 Dec 2019 14:51:42: 3000000 INFO @ Tue, 10 Dec 2019 14:51:43: #1 tag size is determined as 40 bps INFO @ Tue, 10 Dec 2019 14:51:43: #1 tag size = 40 INFO @ Tue, 10 Dec 2019 14:51:43: #1 total tags in treatment: 3159480 INFO @ Tue, 10 Dec 2019 14:51:43: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 14:51:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 14:51:43: #1 tags after filtering in treatment: 3159480 INFO @ Tue, 10 Dec 2019 14:51:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 14:51:43: #1 finished! INFO @ Tue, 10 Dec 2019 14:51:43: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 14:51:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 14:51:43: #2 number of paired peaks: 5 WARNING @ Tue, 10 Dec 2019 14:51:43: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 14:51:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6932541/SRX6932541.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。