Job ID = 14522025 SRX = SRX6917498 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9332695 spots for SRR10197356/SRR10197356.sra Written 9332695 spots for SRR10197356/SRR10197356.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:08 9332695 reads; of these: 9332695 (100.00%) were paired; of these: 239654 (2.57%) aligned concordantly 0 times 7956820 (85.26%) aligned concordantly exactly 1 time 1136221 (12.17%) aligned concordantly >1 times ---- 239654 pairs aligned concordantly 0 times; of these: 64733 (27.01%) aligned discordantly 1 time ---- 174921 pairs aligned 0 times concordantly or discordantly; of these: 349842 mates make up the pairs; of these: 203697 (58.23%) aligned 0 times 108566 (31.03%) aligned exactly 1 time 37579 (10.74%) aligned >1 times 98.91% overall alignment rate Time searching: 00:13:08 Overall time: 00:13:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1529428 / 9057445 = 0.1689 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:27:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:27:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:27:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:27:51: 1000000 INFO @ Sat, 15 Jan 2022 22:28:02: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:28:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:28:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:28:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:28:13: 3000000 INFO @ Sat, 15 Jan 2022 22:28:22: 1000000 INFO @ Sat, 15 Jan 2022 22:28:25: 4000000 INFO @ Sat, 15 Jan 2022 22:28:33: 2000000 INFO @ Sat, 15 Jan 2022 22:28:37: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:28:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:28:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:28:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:28:45: 3000000 INFO @ Sat, 15 Jan 2022 22:28:48: 6000000 INFO @ Sat, 15 Jan 2022 22:28:56: 1000000 INFO @ Sat, 15 Jan 2022 22:28:57: 4000000 INFO @ Sat, 15 Jan 2022 22:29:00: 7000000 INFO @ Sat, 15 Jan 2022 22:29:09: 5000000 INFO @ Sat, 15 Jan 2022 22:29:11: 2000000 INFO @ Sat, 15 Jan 2022 22:29:12: 8000000 INFO @ Sat, 15 Jan 2022 22:29:21: 6000000 INFO @ Sat, 15 Jan 2022 22:29:23: 9000000 INFO @ Sat, 15 Jan 2022 22:29:27: 3000000 INFO @ Sat, 15 Jan 2022 22:29:33: 7000000 INFO @ Sat, 15 Jan 2022 22:29:36: 10000000 INFO @ Sat, 15 Jan 2022 22:29:44: 4000000 INFO @ Sat, 15 Jan 2022 22:29:45: 8000000 INFO @ Sat, 15 Jan 2022 22:29:48: 11000000 INFO @ Sat, 15 Jan 2022 22:29:57: 9000000 INFO @ Sat, 15 Jan 2022 22:30:00: 12000000 INFO @ Sat, 15 Jan 2022 22:30:00: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:30:09: 10000000 INFO @ Sat, 15 Jan 2022 22:30:12: 13000000 INFO @ Sat, 15 Jan 2022 22:30:15: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:30:22: 11000000 INFO @ Sat, 15 Jan 2022 22:30:24: 14000000 INFO @ Sat, 15 Jan 2022 22:30:30: 7000000 INFO @ Sat, 15 Jan 2022 22:30:34: 12000000 INFO @ Sat, 15 Jan 2022 22:30:36: 15000000 INFO @ Sat, 15 Jan 2022 22:30:41: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 22:30:41: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 22:30:41: #1 total tags in treatment: 7564677 INFO @ Sat, 15 Jan 2022 22:30:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:30:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:30:41: #1 tags after filtering in treatment: 4370208 INFO @ Sat, 15 Jan 2022 22:30:41: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 22:30:41: #1 finished! INFO @ Sat, 15 Jan 2022 22:30:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:30:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:30:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:30:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:30:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:30:45: 8000000 INFO @ Sat, 15 Jan 2022 22:30:46: 13000000 INFO @ Sat, 15 Jan 2022 22:30:58: 14000000 INFO @ Sat, 15 Jan 2022 22:31:02: 9000000 INFO @ Sat, 15 Jan 2022 22:31:10: 15000000 INFO @ Sat, 15 Jan 2022 22:31:15: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 22:31:15: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 22:31:15: #1 total tags in treatment: 7564677 INFO @ Sat, 15 Jan 2022 22:31:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:31:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:31:15: #1 tags after filtering in treatment: 4370208 INFO @ Sat, 15 Jan 2022 22:31:15: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 22:31:15: #1 finished! INFO @ Sat, 15 Jan 2022 22:31:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:31:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:31:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:31:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:31:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:31:18: 10000000 INFO @ Sat, 15 Jan 2022 22:31:32: 11000000 INFO @ Sat, 15 Jan 2022 22:31:46: 12000000 INFO @ Sat, 15 Jan 2022 22:32:01: 13000000 INFO @ Sat, 15 Jan 2022 22:32:15: 14000000 INFO @ Sat, 15 Jan 2022 22:32:29: 15000000 INFO @ Sat, 15 Jan 2022 22:32:34: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 22:32:34: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 22:32:34: #1 total tags in treatment: 7564677 INFO @ Sat, 15 Jan 2022 22:32:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:32:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:32:34: #1 tags after filtering in treatment: 4370208 INFO @ Sat, 15 Jan 2022 22:32:34: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 22:32:34: #1 finished! INFO @ Sat, 15 Jan 2022 22:32:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:32:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:32:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:32:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:32:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917498/SRX6917498.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling