Job ID = 14522024 SRX = SRX6917497 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11993632 spots for SRR10197355/SRR10197355.sra Written 11993632 spots for SRR10197355/SRR10197355.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:38 11993632 reads; of these: 11993632 (100.00%) were paired; of these: 361894 (3.02%) aligned concordantly 0 times 10021010 (83.55%) aligned concordantly exactly 1 time 1610728 (13.43%) aligned concordantly >1 times ---- 361894 pairs aligned concordantly 0 times; of these: 42430 (11.72%) aligned discordantly 1 time ---- 319464 pairs aligned 0 times concordantly or discordantly; of these: 638928 mates make up the pairs; of these: 463111 (72.48%) aligned 0 times 135089 (21.14%) aligned exactly 1 time 40728 (6.37%) aligned >1 times 98.07% overall alignment rate Time searching: 00:15:38 Overall time: 00:15:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2571805 / 11541558 = 0.2228 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:32:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:32:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:32:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:32:37: 1000000 INFO @ Sat, 15 Jan 2022 22:32:45: 2000000 INFO @ Sat, 15 Jan 2022 22:32:53: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:32:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:32:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:32:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:33:01: 4000000 INFO @ Sat, 15 Jan 2022 22:33:07: 1000000 INFO @ Sat, 15 Jan 2022 22:33:10: 5000000 INFO @ Sat, 15 Jan 2022 22:33:15: 2000000 INFO @ Sat, 15 Jan 2022 22:33:19: 6000000 INFO @ Sat, 15 Jan 2022 22:33:24: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:33:27: 7000000 INFO @ Sat, 15 Jan 2022 22:33:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:33:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:33:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:33:32: 4000000 INFO @ Sat, 15 Jan 2022 22:33:36: 8000000 INFO @ Sat, 15 Jan 2022 22:33:38: 1000000 INFO @ Sat, 15 Jan 2022 22:33:42: 5000000 INFO @ Sat, 15 Jan 2022 22:33:44: 9000000 INFO @ Sat, 15 Jan 2022 22:33:48: 2000000 INFO @ Sat, 15 Jan 2022 22:33:51: 6000000 INFO @ Sat, 15 Jan 2022 22:33:53: 10000000 INFO @ Sat, 15 Jan 2022 22:33:57: 3000000 INFO @ Sat, 15 Jan 2022 22:34:01: 7000000 INFO @ Sat, 15 Jan 2022 22:34:02: 11000000 INFO @ Sat, 15 Jan 2022 22:34:06: 4000000 INFO @ Sat, 15 Jan 2022 22:34:10: 8000000 INFO @ Sat, 15 Jan 2022 22:34:10: 12000000 INFO @ Sat, 15 Jan 2022 22:34:16: 5000000 INFO @ Sat, 15 Jan 2022 22:34:19: 13000000 INFO @ Sat, 15 Jan 2022 22:34:20: 9000000 INFO @ Sat, 15 Jan 2022 22:34:25: 6000000 INFO @ Sat, 15 Jan 2022 22:34:27: 14000000 INFO @ Sat, 15 Jan 2022 22:34:29: 10000000 INFO @ Sat, 15 Jan 2022 22:34:35: 7000000 INFO @ Sat, 15 Jan 2022 22:34:36: 15000000 INFO @ Sat, 15 Jan 2022 22:34:38: 11000000 INFO @ Sat, 15 Jan 2022 22:34:44: 8000000 INFO @ Sat, 15 Jan 2022 22:34:45: 16000000 INFO @ Sat, 15 Jan 2022 22:34:47: 12000000 INFO @ Sat, 15 Jan 2022 22:34:53: 9000000 INFO @ Sat, 15 Jan 2022 22:34:53: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:34:56: 13000000 INFO @ Sat, 15 Jan 2022 22:35:02: 18000000 INFO @ Sat, 15 Jan 2022 22:35:02: 10000000 INFO @ Sat, 15 Jan 2022 22:35:05: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 22:35:05: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 22:35:05: #1 total tags in treatment: 9061120 INFO @ Sat, 15 Jan 2022 22:35:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:35:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:35:05: #1 tags after filtering in treatment: 5064998 INFO @ Sat, 15 Jan 2022 22:35:05: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 22:35:05: #1 finished! INFO @ Sat, 15 Jan 2022 22:35:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:35:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:35:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:35:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:35:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.05_peaks.narrowPeakINFO @ Sat, 15 Jan 2022 22:35:05: 14000000 : No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:35:11: 11000000 INFO @ Sat, 15 Jan 2022 22:35:14: 15000000 INFO @ Sat, 15 Jan 2022 22:35:20: 12000000 INFO @ Sat, 15 Jan 2022 22:35:23: 16000000 INFO @ Sat, 15 Jan 2022 22:35:28: 13000000 INFO @ Sat, 15 Jan 2022 22:35:31: 17000000 INFO @ Sat, 15 Jan 2022 22:35:37: 14000000 INFO @ Sat, 15 Jan 2022 22:35:40: 18000000 INFO @ Sat, 15 Jan 2022 22:35:43: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 22:35:43: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 22:35:43: #1 total tags in treatment: 9061120 INFO @ Sat, 15 Jan 2022 22:35:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:35:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:35:43: #1 tags after filtering in treatment: 5064998 INFO @ Sat, 15 Jan 2022 22:35:43: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 22:35:43: #1 finished! INFO @ Sat, 15 Jan 2022 22:35:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:35:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:35:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:35:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:35:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:35:45: 15000000 INFO @ Sat, 15 Jan 2022 22:35:53: 16000000 INFO @ Sat, 15 Jan 2022 22:36:00: 17000000 INFO @ Sat, 15 Jan 2022 22:36:08: 18000000 INFO @ Sat, 15 Jan 2022 22:36:11: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 22:36:11: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 22:36:11: #1 total tags in treatment: 9061120 INFO @ Sat, 15 Jan 2022 22:36:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:36:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:36:11: #1 tags after filtering in treatment: 5064998 INFO @ Sat, 15 Jan 2022 22:36:11: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 22:36:11: #1 finished! INFO @ Sat, 15 Jan 2022 22:36:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:36:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:36:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:36:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:36:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX6917497/SRX6917497.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling